Expression pattern of Rheb gene in Jabal Barez Red goat

AZRA NAJMI NOORI; MOHAMMAD REZA BAHREINI BEHZADI; MOHAMMAD REZA MOHAMMADABADI

doi:10.56093/ijans.v87i11.75890

Authors

AZRA NAJMI NOORI Yasouj University, Yasouj 75918–74831 Iran
MOHAMMAD REZA BAHREINI BEHZADI Yasouj University, Yasouj 75918–74831 Iran
MOHAMMAD REZA MOHAMMADABADI Yasouj University, Yasouj 75918–74831 Iran

https://doi.org/10.56093/ijans.v87i11.75890

Keywords:

Expression, Jabal Barez Red goat, Rheb gene, Tissue

Abstract

According to importance of Rheb gene on growth, cell cycle and cancer, expression of this gene for the first time was studied in Jabal Barez Red goat. Rheb belongs to Ras family that encodes a carboxylterminal CAAX box indicating that the protein may undergo post-translational farnesylation. Over-expressions of Rheb simulate cell growth while knockdown of Rheb expression, inhibits protein synthesis and cell growth. In this study, expression of Rheb gene was investigated by Real-Time PCR and Pfaffl method in various tissues including brain (medulla), brain (cortex), heart, kidney (cortex), kidney (medulla), testis, lung, liver and spleen. For analyzing the data of Pfaffl method, SAS software was used. Results showed that the Rheb gene was expressed in all the tested tissues and the highest level of expression was observed in spleen and the lowest level was detected in lung. Therefore, this gene is expressed in all the tissues and physiological effects of this gene needs to be investigated in different tissues and different animals.

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References

Abbaszadeh Mehrabadi A, Mohammadabadi M R, Esmailizadeh K A and Alinaghizadeh R. 2011. Polymorphism studying exon 2 of GoLA-DRB3 gene in Nadoushan goat using PCR-RFLP. Animal Science Researches of Iran 3: 274–79.

Buerger C, DeVries B and Stambolic V. 2006. Localization of Rheb to the endomembrane is critical for its signaling function. Biochemical and Biophysical Research Communications 344: 869–80. DOI: https://doi.org/10.1016/j.bbrc.2006.03.220

Herrmann S and Wortmann F J. 1997. Opportunities for the simultaneous estimation of essential fleece parameters in raw cashmere fleeces. Livestock Production Science 48: 1–12. DOI: https://doi.org/10.1016/S0301-6226(96)01002-0

Inoki K, Li Y and Xu T. 2003. Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Development 17: 1829–34. DOI: https://doi.org/10.1101/gad.1110003

Long X, Lin Y, Ortiz-Vega S, Busch S and Avruch J. 2007. The Rheb switch 2 segment is critical for signaling to target of Rapamycin Complex. Journal of Biological Chemistry 282(25): 18542–51. DOI: https://doi.org/10.1074/jbc.M610736200

Ma D, Bai X, Guo S and Jiang Y. 2008. The switch I region of Rheb is critical for its interaction with FKBP38. Journal of Biological Chemistry 283(38): 25963–70. DOI: https://doi.org/10.1074/jbc.M802356200

Mahdian R, Kamyab A R and Amirzargar A A. 2014. Real-Time PCR basic and application. First ed., Artin Teb, Tehran, Iran. 285 pp.

Saucedo L J, Gao X, Chiarelli D A, Li L, Pan D and Edgar B A. 2003. Rheb promotes cell growth as a component of the insulin/ TOR signaling network. Natural Cell Biology 5: 566–71. DOI: https://doi.org/10.1038/ncb996

Tohidinezhad F, Mohammadabadi M R, Esmailizadeh A K and Najmi Noori A. 2014. Comparison of different levels of Rheb gene expression in different tissues of Raini Cashmir goat. Journal of Agricultural Biotechnology 6(4): 37–50.

Yamagata K, Sanders L K, Kaufmann W E, Yee W, Barnes C A, Nathan D and Worley P F. 1994. Rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein. Journal of Biological Chemistry 269: 16333–39. DOI: https://doi.org/10.1016/S0021-9258(17)34012-7

Zheng X U, Yang J F, Wang X J, Liang Y, Wu M L, Shi J J, Zhang T, Qin Y, Li S Y, Hao X Y, Wang Z G and Liu D J. 2011. Molecular characterization and expression pattern of Rheb gene in inner Mongolia Cashmere goat (Capra hircus). Agricultural Sciences in China 10(9): 1452–58. DOI: https://doi.org/10.1016/S1671-2927(11)60138-7