Anti-apoptotic effect of melatonin in sperm of mithun
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Keywords:
Apoptosis, Melatonin, Mithun, SpermAbstract
The present study was designed to measure the effect of melatonin (MT) on apoptosis of sperm in mithun. Ejaculates (20) were collected from mithun bulls and were split into five equal aliquots, diluted with the TEYC extender. (Gr 1: semen without additives (control), Gr 2 to Gr 6: semen was diluted with 1, 2, 3, 4 and 5 mM of MT respectively). Apoptotic sperm percentage was estimated using commercially available apoptotic assessment kit at immediately after dilution, equilibration and post-thaw. Inclusion of MT into diluent resulted in a significant decrease in apoptotic sperm percentage at different stage of semen preservation as compared to untreated control group. Moreover, MT at 3 mM had significantly lower apoptotic sperm percentage than MT at 1 mM, 2 mM or 4 mM or 5 mM stored sperm at different stage. It was concluded that MT at 3 mM is suitable to minimize apoptosis in mithun sperm to pursuit future sperm preservation protocols.
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References
Alvarez J G and Storey B T. 1992. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a model of sub lethal cryo damage to human sperm during cryopreservation. Journal of Andrology 13(3): 232–41.
Ashrafi I, Kohram H and Ardabili F F. 2013. Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa. Animal Reproduction Science 139(1–4): 25–30. DOI: https://doi.org/10.1016/j.anireprosci.2013.03.016
Bora B, Savino N, Baruah K K, Mukherjee A and Perumal P. 2015. Effect of cryopreservation on apoptosis of mithun (Bos frontalis) spermatozoa. Indian Veterinary Journal 92(2): 66– 68.
Casao A, Mendoza N, Perez-Pe R, Grasa P, Abecia J A, Forcada F, Cebrián-Perez J A and Muino-Blanco T. 2009. Melatonin prevents capacitation and apoptotic-like changes of ram spermatozoa and increases fertility rate. Journal of Pineal Research 48(1): 39–46. DOI: https://doi.org/10.1111/j.1600-079X.2009.00722.x
Gavella M, Lipovac V, Vucic M and Rocic B. 1996. Relationship of sperm superoxide dismutase-like activity with other sperm specific enzymes and experimentally induced lipid peroxidation in infertile men. Andrologia 28(4): 222–29. DOI: https://doi.org/10.1111/j.1439-0272.1996.tb02787.x
Hardeland R, Reiter R J, Poeggeler B and Tan D X. 1993. The significance of the metabolism of the neurohormone melatonin: Antioxidative protection and formation of bioactive substances. Neuroscience and Biobehavioral Reviews 17(3): 347–57. DOI: https://doi.org/10.1016/S0149-7634(05)80016-8
Hyun-Yong J, Sung-Gon K, Jong-Taek K, Choon-Keun P, Hee- Tae C, Hak-Kyu L and Boo-Keun Y. 2006. Effects of antioxidants on sperm motility during in vitro storage of boar semen. Korean Journal of Gerontology 16(6): 47–51.
Karbownik M and Reiter R J. 2000. Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation. Proceedings of the Society for Experimental Biology and Medicine 225(1): 9–22. DOI: https://doi.org/10.1046/j.1525-1373.2000.22502.x
Khan D R, Ahmad N, Anzar M and Channa A A. 2009. Apoptosis in fresh and cryopreserved buffalo sperm. Theriogenology 71(5): 872–76. DOI: https://doi.org/10.1016/j.theriogenology.2008.09.056
Lopez A, Garcia J A, Escames G, Venegas C, Ortiz F, Lopez L C and Acuna- Castroviejo D. 2009. Melatonin protects the mitochondria from oxidative damage reducing oxygen consumption, membrane potential, and superoxide anion production. Journal of Pineal Research 46(2): 188–98. DOI: https://doi.org/10.1111/j.1600-079X.2008.00647.x
Martin G, Sabido O, Durand P and Levy R. 2004. Cryopreservation induces an apoptosis-like mechanism in bull sperm. Biology of Reproduction 71(1): 28–37. DOI: https://doi.org/10.1095/biolreprod.103.024281
Maxwell W M C and Stojanov T. 1996. Liquid storage of ram semen in the absence or presence of some antioxidants. Reproduction, Fertility and Development 8(6): 1013–20. DOI: https://doi.org/10.1071/RD9961013
Reiter R J, Tan D X, Kim S J and Qi W. 1998. Melatonin as a pharmacological agent against damage to lipids and DNA. Proceedings of Western Pharmacology Society 41: 229–36.
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