Immunolocalization of estrogen alpha and progesterone beta receptors in goat mammary gland

RATAN KUMAR CHOUDHARY; DEVENDRA PATHAK; SHANTI CHOUDHARY; RAMNEEK VERMA

doi:10.56093/ijans.v88i4.78803

Authors

RATAN KUMAR CHOUDHARY Assistant Professor, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
DEVENDRA PATHAK Assistant Scientist, Department of Veterinary Anatomy, College of Veterinary Sciences
SHANTI CHOUDHARY Research Associate, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
RAMNEEK VERMA Professor and Director, School of Animal Biotechnology

https://doi.org/10.56093/ijans.v88i4.78803

Keywords:

ER-alpha, Goat mammary gland, Mammary precancer, PR-B

Abstract

Steroid receptors particularly estrogen receptor alpha and progesterone receptor beta are essential for mammary gland development. Objective of this study was to explore transcript and protein expression profile of steroid receptors in goat mammary glands. A varied expression of ER-alpha and PR-B was observed during lactation, nonlactating/ dry, mastitic and mammary pre-cancer/cancer in goats. During lactation, immunopositivity of ER-alpha was observed only in the nuclei of mammary epithelial cells (MEC) and stromal cells. However, in non-lactating stage, ER-alpha immunopositivity was observed both in nucleus and cytoplasm of MEC. In mammary pre-cancer (based on aberrant expression of CD10, FNDC3B and MUC1) immunoreactivity of ER-alpha (38±12.5%) varied from non-lactating (14.8±3.1%) and lactating (7.9±2.6%) glands. During naturally infected mastitis, a reduction in the expression of ER-alpha and PR-B was observed.We observed similar expression patterns of ER-alpha and PR-B as that of their protein expression. Transcripts of these receptors were highest in mammary precancer. In comparison to lactating glands, expressions of ER-alpha and PR-B was upregulated in mammary precancers by 17- folds and 9.2-folds, respectively. These results showed a reduction in expression of steroid receptors in mastitic glands and upregulation in mammary precancer indicating role of these receptors in cell proliferation.

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