Phylogenetic studies on RoTat 1.2 VSG of Trypanosoma evansi isolate from semi arid India

ANJALI DEVI; DAYA SHANKER; VIKRANT SUDAN; AMIT JAISWAL; AMIT SINGH; MAHENDRA KUMAR CHAUDHARY

doi:10.56093/ijans.v88i2.79290

Authors

ANJALI DEVI Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry
DAYA SHANKER Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry
VIKRANT SUDAN Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry
AMIT JAISWAL Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry
AMIT SINGH Department of Ve terinary Parasitology, College of Veterinary Sciences and Animal Husbandry
MAHENDRA KUMAR CHAUDHARY Pt Deendayal Upadhyay Veterinary and Animal Science University, Mathura, Uttar Pradesh 281 001 India

https://doi.org/10.56093/ijans.v88i2.79290

Keywords:

India, Molecular characterization, Phylogenetic studies, Rotat 1.2 VSG, Trypanosoma evansi

Abstract

RoTat 1.2 variant surface glycoprotein (VSG) is considered to be one of the most important VSGs expressed in many of the isolates of Trypanosoma evansi. This accounts for its utility as a potent molecule for both molecular and serological based detection of Surra. Of late, there are reports of its absence and variation from a fewer isolates of T. evansi. The study of these variations by means of molecular phylogenetic studies is of immense significance in determining the evolutionary phylogeny of these T. evansi isolates. With this information in the background, the present study was designed to clone and characterize RoTat 1.2 VSG from horse isolate of T. evansi from semi arid regions of India. There after, the gene was compared with various other isolates across the world. Interestingly, theisolate was found to be closer to camel isolates from Egypt than the other known isolates from India and Kenya. The finding is important from evolutionary point of view.

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