Expression of Mn-SOD and Cu,Zn-SOD of Sahiwal and Karan-Fries in response to acute heat exposure

P MAYENGBAM; T C TOLENKHOMBA; R C UPADHYAY

doi:10.56093/ijans.v88i2.79328

Authors

P MAYENGBAM Assistant Professor, Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, India
T C TOLENKHOMBA Assistant Professor (SS), Department of Animal Breeding and Genetics, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, India
R C UPADHYAY Ex. PI. NICRA and Head, Dairy Cattle Physiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v88i2.79328

Keywords:

Cu, Zn-SOD, Heat stress, Karan-Fries, Mn-SOD, Sahiwal

Abstract

The present study aimed to investigate the expression of Mn-SOD and Cu,Zn-SOD mRNA in lymphocytes of Sahiwal and Karan-Fries during acute thermal exposure. Six numbers each of Sahiwal and Karan-Fries heifers in the age group of 2–2.5 years were exposed at 40±1°C and 50% relative humidity inside a psychrometric chamber for 4 h in a day. Respiration rate, heart rate and rectal temperatures were recorded to calculate heat tolerance index by calculating Dairy Search Index (DSI). Blood samples were collected at 0, 1, 2, 3 and 4 h of heat exposure for estimation of Mn-SOD and Cu,Zn-SOD mRNA expression by RT-PCR. DSI of Sahiwal was not influenced by heat exposure even after 4 h of heat exposure. After 1 h of heat exposure, DSI of Karan-Fries increased which further escalated after 3 h of exposure in Karan-Fries. Mn-SOD expression increased after 4 h and 2 h of heat exposure in Sahiwal and Karan-Fries respectively, however the expression declined after 3 h of exposure in Karan-Fries. Expression of Cu,Zn-SOD mRNA of Sahiwal and Karan-Fries was not influenced by heat exposure. The study revealed better equipped antioxidant system of Sahiwal than Karan-Fries in response to acute thermal exposure.

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