Comparative sensitivity of sandwich ELISA, RT-PCR, and real-time RT-PCR for detection of bluetongue virus


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Authors

  • KARAM CHAND Scientist, Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Nainital, Uttarakhand 263 138 India
  • S K BISWAS Scientist, Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Nainital, Uttarakhand 263 138 India
  • B MONDAL Principal Scientist, Eastern Regional Station, Indian Veterinary Research Institute, Kolkata

https://doi.org/10.56093/ijans.v88i2.79330

Keywords:

Bluetongue virus, RT-PCR, Real-time RT-PCR, Sandwich-ELISA, Sensitivity

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References

Afshar A. 1998. Bluetongue: laboratory diagnosis. Comparative Immunology Microbiology and Infectious Diseases 17(3–4): 221–42. DOI: https://doi.org/10.1016/0147-9571(94)90045-0

Chand K, Biswas S K, De A, Sing B and Mondal B. 2009. A polyclonal antibody-based sandwich ELISA for the detection of bluetongue virus in cell culture and blood of sheep infected experimentally. Journal of Virological Methods 160(1): 189– 92. DOI: https://doi.org/10.1016/j.jviromet.2009.04.032

Chand K, Biswas S K, Pandey A B, Muthuchelvan D and Mondal B. 2015. Bluetongue in India: A review. Advances in Animal and Veterinary Sciences 3(11): 605–12. DOI: https://doi.org/10.14737/journal.aavs/2015/3.11.605.612

Chand K, Biswas S K, Pandey A B, Saxena A, Tewari N and Mondal B. 2017. A competitive ELISA for detection of group specific antibody to bluetongue virus using anti-core antibody. Biologicals 46: 168–71. DOI: https://doi.org/10.1016/j.biologicals.2017.01.002

Dangler C A, De Mattos C A, De Mattos C C and Osburn B I. 1990. Identifying bluetongue virus ribonucleic acid sequence by polymerase chain reaction. Journal of Virological Methods 28(3): 281–92. DOI: https://doi.org/10.1016/0166-0934(90)90121-U

Leblanc N, Rasmussen T B, Fernández J, Sailleau C, Rasmussen L D, Uttenthal Å, Zientara S, Belák S and Hakhverdyan M. 2010. Development of a real-time RT-PCR assay based on primer–probe energy transfer for the detection of all serotypes of bluetongue virus. Journal of Virological Methods 167(2): 165–71. DOI: https://doi.org/10.1016/j.jviromet.2010.03.032

Maan S, Maan N S, Belaganahalli M N, Potgieter A C, Kumar V, Batra K, Wright I M, Kirkland P D and Mertens P P. 2016. Development and evaluation of real time RT-PCR assays for detection and typing of Bluetongue virus. PLoS One 11(9): e0163014. DOI: https://doi.org/10.1371/journal.pone.0163014

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OIE. 2004. Manual of Standards for Diagnostic Tests and Vaccines for Terrestrial Animals. 5th edn. pp. 197-210.

Polci A, Camma C, Serini S, Di Gialleonardo L, Monaco F and Savini G. 2007. Real-time polymerase chain reaction to detect bluetongue virus in blood samples. Veterinaria Italiana 43(1): 77–87.

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Prasad G, Minakshi, Malik Y and Maan S. 1999. RT-PCR and its detection limit for cell culture grown bluetongue virus using NS1 gene group specific primers. Indian Journal of Experimental Biology 37(12): 1255–58.

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Submitted

2018-05-02

Published

2018-05-02

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Short-Communication

How to Cite

CHAND, K., BISWAS, S. K., & MONDAL, B. (2018). Comparative sensitivity of sandwich ELISA, RT-PCR, and real-time RT-PCR for detection of bluetongue virus. The Indian Journal of Animal Sciences, 88(2), 191-193. https://doi.org/10.56093/ijans.v88i2.79330
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