Polymorphism of keratin-associated protein (KAP) 7 gene and its association with wool traits in Rambouillet sheep

VIKAS MAHAJAN; A K DAS; R K TAGGAR; DHIRENDRA KUMAR; RAJAN SHARMA

doi:10.56093/ijans.v88i2.79339

Authors

VIKAS MAHAJAN
A K DAS Principal Scientist, Animal Genetics & Breeding Section, ICAR-Central Institute for Research on Cattle, Grass Farm Raod, Meerut Cantt, Meerut
R K TAGGAR
DHIRENDRA KUMAR Assistant Professor, Division of Animal Genetics and Breeding, Faculty of Veterinary Science and Animal Husbandry, SKUAST-Jammu, Jammu and Kashmir 181 102 India
RAJAN SHARMA Veterinary Assistant Surgeon, Department of Animal Husbandry, Kishtwar

https://doi.org/10.56093/ijans.v88i2.79339

Keywords:

Keratin associated protein 7, PCR-SSCP, Polymorphisms, Rambouillet sheep, Wool traits

Abstract

In the present study, association of polymorphic variants of KAP 7 gene with wool traits in Rambouillet sheep was investigated by using SSCP-PCR. Genomic DNA was isolated from blood samples of 100 Rambouillet sheep. A 388 bp segment was amplified by PCR using ovine specific primers for KAP 7 gene. The results identified 3 genotypes, viz. AA, AB and BB with genotype frequencies of 0.340 (34), 0.270 (27) and 0.390 (39) respectively and allele frequencies of 0.475 for allele A and 0.525 for allele B. The χ²-test showed that the genotype distribution was not in agreement with Hardy-Weinberg equilibrium (HWE). KAP 7 gene has significantly higher clean wool yield and greasy fleece weight in males whereas females have significantly superior fibre diameter, higher staple length and wool count. BB genotype has significantly higher staple length followed by AA and AB genotype. BB genotype has significantly higher greasy fleece weight production followed by AB and least by AA genotype. From the study, it may be concluded that KAP 7 gene might be a potential molecular marker for genetic selection for higher staple length and greasy fleece weight in Rambouillet sheep.

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Author Biographies

VIKAS MAHAJAN

Assistant Professor, Division of Animal Genetics and Breeding, Faculty of Veterinary Science and Animal Husbandry, SKUAST-Jammu, Jammu and Kashmir 181 102 India
R K TAGGAR

Professor and Head, Division of Animal Genetics and Breeding, Faculty of Veterinary Science and Animal Husbandry, SKUAST-Jammu, Jammu and Kashmir 181 102 India

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