DNA barcoding of some commonly exploited fishes from the northern Western Ghats, India

UBAID QAYOOM; RAVINDRA A PAWAR; SWAPNAJA A MOHITE; MILIND S SAWANT; VIVEK H NIRMALE; SHRIKANT P PAWAR; MUKUNDA GOSWAMI; WAZIR S LAKRA

doi:10.56093/ijans.v88i2.79354

Authors

UBAID QAYOOM ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 India
RAVINDRA A PAWAR Associate Professor, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 India
SWAPNAJA A MOHITE Associate Professor, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 India
MILIND S SAWANT Associate Professor, Department of Fisheries Hydrography, College of Fisheries
VIVEK H NIRMALE Assisstant Professor, Department of Fisheries Biology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 India
SHRIKANT P PAWAR Technical Officer, National Centr for Cell Science, Pune
MUKUNDA GOSWAMI Principal Scientist, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 India
WAZIR S LAKRA Former Director, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra 400 061 India

https://doi.org/10.56093/ijans.v88i2.79354

Keywords:

DNA barcoding, Freshwater fish, COI, Molecular phylogeny, Western Ghats

Abstract

The Western Ghats, being very rich in freshwater fish diversity, has recently been confirmed as a globally
significant centre of diversity and endemism for freshwater species and comprise one of the 34 global biodiversity
hotspots. Owing to its extreme ichthyofaunal diversity, the present study was designed to generate cytochrome
oxidase I (COI) DNA barcodes for the identification of some commonly exploited fishes from the west-flowing
rivers of northern Western Ghats. Twenty-three fish specimens representing 6 families and 10 species were barcoded
from the major west-flowing rivers of the northern Western Ghats. The obtained barcodes discriminated all the
species with sufficient barcode gap. The average Kimura two parameter (K2P) values for within species, the genus
and family distances were 0.37, 17.74 and 18.51% respectively. The neighbour-joining tree revealed distinct clusters
corresponding to the taxonomic status of the species. Generated barcodes are expected to provide the much-needed
baseline reference for the ichthyofaunal biodiversity of the global biodiversity hotspot.

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