Phylogenetic analysis of sheep pox virus (SPPV) virion core protein P4a gene revealed extensive sequence conservation among capripox viruses

BINA MISHRA; G RAVI KUMAR; SONAL SONAL; C L PATEL; V K CHATURVEDI

doi:10.56093/ijans.v88i1.79420

Authors

BINA MISHRA Principal Scientist, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122 India
G RAVI KUMAR Principal Scientist, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122 India
SONAL SONAL Scientist, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122 India
C L PATEL Scientist, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122 India
V K CHATURVEDI Principal Scientist, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v88i1.79420

Keywords:

Capripox viruses, Phylogenetic analysis, Sheep pox virus, Virion core protein P4a gene

Abstract

In the present study, virion core protein P4a gene was PCR amplified from sheep pox virus (SPPV) Jaipur isolate and Roumanian Fanar (RF) vaccine strain adapted and propagated in lamb testis/vero cells. Gene specific primers were designed for amplification of P4a gene. Amplified P4a gene fragment was sequence characterized and 808 bp sequence was compared across SPPV, GTPV and LSDV isolates available in GenBank database which revealed extensive sequence conservation of 97% to 100% within pox virus groups. Sheep pox virus Jaipur isolate was found closely placed with Roumaninan Fanar (RF) and TU isolates. Further, phylogenetic analysis of P4a gene sequence indicated three distinct clusters of Capripox viruses with GTPV interestingly placed closely to LSDV group.

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