Haematological variations in visually anaemic sheep naturally infected with Haemonchus contortus in farm conditions at arid Rajasthan
Keywords:Anaemia, Haematology, Haemonchus contortus, Rajasthan, Sheep, Targeted selective treatment
With an objective to reduce use of anthelmintic frequency, targeted selective treatment was implemented for farm flocks in arid Rajasthan. An eye color chart developed by CSWRI, Avikanagar was used for screening the flocks at monthly interval from July to March each year (from 2008 to 2016). All the scorings were done on the same day along with collection of faecal and blood samples from visually anaemic sheep and estimated faecal egg counts (FECs) and erythron parameters, respectively. Data generated were used to establish relationship among haematological estimates and intensity of strongyle infection in visually anaemic sheep. Out of 687 visually anaemic sheep, maximum proportion (54.1%) was recorded in monsoon (Jul-Sep). The frequency distribution exhibited a maximum of 36.3% of visually anaemic sheep with high level (>2001 epg) of strongyle infection. The mean intensity of strongyle infection in visually anaemic sheep varied significantly (P<0.001) from nil (nil epg group) to 8631.6±491.3 epg (>2001 epg group). A significant (P<0.001) influence of strongyle infection level was observed on Hb, PCV and mean corpuscular haemoglobin concentration (MCHC). The overall Hb concentration varied (P<0.001) from 5.7 (>2001 epg) to 7.2g% (nil epg). The overall magnitude of PCV exhibited a linear decline with an increased level of infection and varied (P<0.001) from 15.7 (>2001 epg) to 20.5% (nil epg). MCHC showed a marginal but significant (P<0.001) increase in sheep with >2000 epg compared to other groups. The Pearson correlation coefficient exhibited negative correlation between intensity of strongyle infection and haematological parameters like HB, PCV and TEC in all FEC levels, but it was significant (P<0.001/0.05) only in sheep with FEC >2001 epg. Periodic monitoring of intensity of worm infection and status of anaemia in host animals are an important part of parasite management programmes which aim to avoid both serious parasitism and excessive chemical treatments.
Abay G, Demissie T, Fekadu A and Teklue T. 2015. Sheep infection by Haemonchus species: effect on haematocrit and evaluation of the FAMACHA© method in Arsi Negele District, Oromia, Ethiopia. Animal and Veterinary Sciences 3: 74–79. DOI: https://doi.org/10.11648/j.avs.20150302.17
Albers G A A, Gray G D, Le Jambre L F, Piper L R, Barger I A and Barker J S F. 1989. The effect of Haemonchus contortus on live weight gain and wool growth in young Merino sheep. Australian Journal of Agricultural Research 40: 419–32. DOI: https://doi.org/10.1071/AR9890419
Altizer S, Dobson A, Hosseini P, Hudson P, Pascual M and Rohani P. 2009. Seasonality and the dynamics of infectious diseases. Ecology Letters 9: 467–84. DOI: https://doi.org/10.1111/j.1461-0248.2005.00879.x
Barger I A and Cox H W. 1984. Wool production of sheep chronically infected with Haemonchus contortus. Veterinary Parasitology 15: 169–75. DOI: https://doi.org/10.1016/0304-4017(84)90033-5
Besier R B, Kahn L P, Sargison N D and van Wyk J A. 2016. Diagnosis, treatment and management of Haemonchus contortus in small ruminants. Advances in Parasitology, Haemonchus contortus and Haemonchosis – Past, Present and Future Trends. (Eds) Gasser R B and von Samson- Himmelstjerna G. Academic Press 93: 181–38. DOI: https://doi.org/10.1016/bs.apar.2016.02.024
Bisset S A, van Wyk J A, Bath G F, Morris C A, Stenson M O and Malan F S. 2001. Phenotypic and genetic relationships amongst FAMACHA score, faecal egg count and performance data in Merino sheep exposed to Haemonchus contortus infection in South Africa. Proceedings of the 5th International Sheep Veterinary Congress, 22–25 January, Stellenbosch, South Africa.
Bordoloi G, Jas R and Ghosh J D. 2012. Changes in the haematobiochemical pattern due to experimentally induced haemonchosis in Sahabadi sheep. Journal of Parasitic Diseases 36: 101–05. DOI: https://doi.org/10.1007/s12639-011-0079-7
Costa C A F, da Vieira L S, Berne M E A, Silva M U D, Guidoni A L and Figueiredo E A P. 2000. Variability of resistance in goats infected with Haemochus contortus in Brazil. Veterinary Parasitology 88: 153–58. DOI: https://doi.org/10.1016/S0304-4017(99)00207-1
Diaz-Anaya A M, Arias-Gonzalez H A, García-Corredor D J and Pulido-Medellín M O. 2014. Estimation of hematocrit packed cell volume and hemoglobin with the presence of Haemonchus sp. in sheep of Oicata, Colombia. Revista de la Facultad de Ciencias Veterinarias 55: 18–24.
Ejlertsen M, Githigia S M, Otieno R O and Thamsborg S M. 2006. Accuracy of an anaemia scoring chart applied on goats in subhumid Kenya and its potential for control of Haemonchus contortus infections. Veterinary Parasitology 141: 291–301. DOI: https://doi.org/10.1016/j.vetpar.2006.05.020
Hoste H, Lefrileux Y and Pommaret A. 2001. Distribution and repeatability of faecal egg counts and blood parameters in dairy goats naturally infected with gastrointestinal nematodes. Research in Veterinary Science 70: 57–60. DOI: https://doi.org/10.1053/rvsc.2000.0442
Jasmer D, Lahmers K K and Brown W C. 2007. Haemonchus contortus intestine: a prominent source of mucosal antigens. Parasite Immunology 29: 139–51. DOI: https://doi.org/10.1111/j.1365-3024.2006.00928.x
Kaplan R M, Burke J M, Terrill T H, Millar J E, Getz W R, Mobini S, Valencia E, Williams M G, Williamson L H, Larsen M and Vatta A F. 2004. Validation of the FAMACH© eye color chart for detecting clinical anemia in sheep and goats on farms in the Southern United States. Veterinary Parasitology 123: 105– 20. DOI: https://doi.org/10.1016/j.vetpar.2004.06.005
Kasali O, Njau B C and Bekele T. 1988. Controlling livestock diseases in the tropics breeding: A perspective. Increasing small ruminant productivity in semi-arid areas. (Eds) Thomson E F and Thomson F S. Kluwer Academic Publications, The Netherland, 47: 237–42. DOI: https://doi.org/10.1007/978-94-009-1317-2_20
Kelkele F A, Tolossa Y H and Kassa G M. 2012. Experimental infection of Ethiopian highland sheep by different infective doses of Haemonchus contortus (L3): haematological and parasitological parameters, serum protein concentrations and clinical responses. Ethiopian Veterinary Journal 16: 41–57. DOI: https://doi.org/10.4314/evj.v16i1.4
Kumari S, Sinha S R P, Sinha S, Kumar A, Kumar P and Ali I. 2013. Haematobiochemical changes in sheep and goats during natural infection of Haemonchus contortus. Indian Journal of Field Veterinarians 8: 43–46.
Le Jambre L F. 1995. Relationship of blood loss to worm numbers, biomass and egg production in haemonchus infected sheep. International Journal for Parasitology 25: 269–73. DOI: https://doi.org/10.1016/0020-7519(94)00118-8
MAFF. 1986. Manual of Veterinary Parasitological Techniques. Vol. 18. Ministry of Agriculture, Fisheries and Food, London. Martin L B, Weil Z M and Nelson R J. 2008. Seasonal changes in vertebrate immune activity: Mediation by physiological tradeoffs. Philosophical Transactions of the Royal Society B Biological Sciences 363: 321–39. DOI: https://doi.org/10.1098/rstb.2007.2142
Roberts J L and Swan R A. 1982. Quantitative studies of ovine haemonchosis. 2. Relationship between total worm counts of Haemonchus contortus, haemoglobin values and body weight. Veterinary Parasitology 9: 217–22. DOI: https://doi.org/10.1016/0304-4017(82)90065-6
Schalm O W, Jain N C and Carroll E J. 1975. Veterinary Haematology. 3rd edn., Lea and Febiger, Philladelphia, USA, pp 15–81.
Scheuerle M, Mahling M, Muntwyler J and Pfister K. 2010. The accuracy of the FAMACHA© method in detecting anaemia and haemonchosis in goat flocks in Switzerland under field conditions. Veterinary Parasitology 170: 71–77. DOI: https://doi.org/10.1016/j.vetpar.2010.01.035
Singh D and Swarnkar C P. 2012. Evaluation of targeted selective treatment strategy in sheep farm of Rajasthan. Indian Journal of Animal Sciences 82: 679–86.
Singh D and Swarnkar C P. 2013. Comparative impact of conventional and strategic worm management schemes in sheep flocks of arid Rajasthan. Indian Journal of Animal Sciences 83: 1128–34.
Singh D, Swarnkar C P, Prince L L L and Pathak K M L. 2011. Economic Analysis and Impact of Gastrointestinal Nematodes on Sheep Production in Rajasthan. Directorate of Knowledge Management in Agriculture, ICAR, New Delhi, pp 1–84.
Swarnkar C P and Singh D. 2011. Role of bioclimatographs in forecasting of strongyle infection in Rajasthan. Indian Journal of Animal Sciences 81: 216–23.
Swarnkar C P and Singh D. 2014. Sensitivity and specificity analysis for targeted selective treatment using eye colour chart in sheep flocks naturally infected with Haemonchus contortus in Rajasthan. Indian Journal of Animal Sciences 84: 1177– 80.
Swarnkar C P, Singh D, Krishna Lal and Khan F A. 2008. Epidemiology and Management of Gastrointestinal Parasites of Sheep flocks in Rajasthan. Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, pp 1–145.
Urquhart G M, Armour J, Dunca J L, Dunn A M and Jennings F W. 2000. Veterinary Parasitology. 2nd edn., Blackwell Science Ltd., London.
van Wyk J A, Hoste H, Kaplan R M and Besier R B. 2006. Targeted selective treatment for worm management-how do we sell rational programs to farmers? Veterinary Parasitology 139: 336–46. DOI: https://doi.org/10.1016/j.vetpar.2006.04.023
Vatta A F, Letty B A, van der Linde M J, van Wijk E F, Hansen J W and Krecek R C. 2001. Testing for clinical anaemia caused by Haemonchus spp. in goats farmed under resource-poor conditions in South Africa using an eye color chart developed for sheep. Veterinary Parasitology 99: 1–14. DOI: https://doi.org/10.1016/S0304-4017(01)00446-0
Yadav C L, Grewall H S and Banerjee D P. 1993. Susceptibility of two cross breeds of sheep to Haemonchus contortus. International Journal for Parasitology 23: 819–22. DOI: https://doi.org/10.1016/0020-7519(93)90082-A
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