Pharmacological characterization of store-operated calcium channels (SOCC) in myometrium of non-pregnant buffaloes (Bubalus bubalis)

ABHISHEK SHARMA; UDAYRAJ P NAKADE; SOUMEN CHOUDHURY; RAJKUMAR SINGH YADAV; SATISH KUMAR GARG

doi:10.56093/ijans.v88i1.79485

Authors

ABHISHEK SHARMA PhD Scholar, Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, DUVASU, Mathura, Uttar Pradesh 281 001 India
UDAYRAJ P NAKADE PhD Scholar, Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, DUVASU, Mathura, Uttar Pradesh 281 001 India
SOUMEN CHOUDHURY Assistant Professor, Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, DUVASU, Mathura, Uttar Pradesh 281 001 India
RAJKUMAR SINGH YADAV Assistant Professor, Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, DUVASU, Mathura, Uttar Pradesh 281 001 India
SATISH KUMAR GARG Professor and Head, Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, DUVASU, Mathura, Uttar Pradesh 281 001 India

https://doi.org/10.56093/ijans.v88i1.79485

Keywords:

2-APB, Buffalo myometrium, Calcium chloride, Store-operated Ca2 Channel

Abstract

Present study unravels the existence and functional involvement of store-operated calcium channels in myometrium of non-pregnant buffaloes. Uteri along with ovaries were collected from nondescript adult cyclic buffaloes immediately after their slaughter from the local abattoir. Under a resting tension of 2 gm, effect of CaCl₂ in the absence and presence of different blockers/modulators of calcium regulatory pathways was recorded. CaCl₂ produced concentration-dependent contraction and the DRCs of CaCl₂ were significantly (P < 0.05) shifted to right in the presence of nifedipine (1 μM) + CPA (10 μM) and nifedipine (1 μM) + CPA (10 μM) + 2-APB (10 μM). After incubation of myometrial strips with nifedipine + CPA in Ca2+ free RLS (-Ca2+), histamine was added to the tissue bath to allow the release of Ca²⁺ from SR while having already blocked the SERCA by CPA (10 μM) to prevent the Ca²⁺ reuptake into SR and nifedipine was used to prevent entry of Ca²⁺ from VDCC when calcium chloride was added and 2-aminoethoxydiphenyl borate (2-APB) (10 μM) was used as a non-specific blocker of store-operated calcium channels (SOCC). In the presence of nifedipine + CPA + 2-APB, calcium chloride produced contractile effect and the maximal contraction observed was only 0.62±0.14 g (n=6) which was significantly (P<0.05) lower compared to that of 1.20±0.10 g (n=6) in the presence of nifedipine + CPA in normal Ca²⁺ free RLS. This observation indicated that after depletion of Ca²⁺ from Sarcoplasmic reticular, SOCC got activated and in the presence of 2- APB, response was significantly reduced. Thus implying the functional involvement of store-operated calcium channels in myometrium of non-pregnant buffaloes.

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