Effect of supplementation of niacin on physiological and blood biochemical parameters in crossbred cows during heat stress
Keywords:Crossbred cows, Heat stress, Niacin, Physiological and blood biochemical parameters
In order to investigate the effect of different levels of niacin supplementation on physiological and blood biochemical parameters during heat stress period (April to August; 120 days), eighteen crossbred early lactating cows (2nd to 4th lactation; 11.56±1.74 days in milk) were divided into three groups of six animals each. The basal ration was fed same to all cows (Green fodder, straw and concentrate was fed as per NRC, 2001), except the addition of niacin @ 600 and 800 mg/kg dry matter intake (DMI) in T1 and T2 groups, respectively. The temperature humidity index (THI) was calculated at 07:30 am and 02:30 pm daily. Fortnightly physiological parameters were recorded and blood was collected from the jugular vein at day 0 and subsequently at 15–day interval from all the experimental animals to study blood biochemical parameters. The results revealed that animals were in either moderate or severe stress at morning, whereas during afternoon the animals were in very severe stress during the entire trial. Supplementation of niacin @ 800 ppm niacin decreased significantly skin temperature, respiration rate, cortisol, super oxide dismutase (SOD) and catalase (P<0.05). Plasma NEFA was significantly low in both niacin supplemented groups (P<0.05). Plasma urea was found significantly (P<0.05) high in T2 (26.59) in comparison to control (24.90) and T1 (25.37). It is concluded that 800 ppm niacin supplementation to lactating crossbred cows resulted in better stress alleviation as indicated by the improved biomarker values viz., SOD, catalase, cortisol and skin temperature.
Banerjee D and Ashutosh. 2010. Circadian changes in physiological responses and blood ionized sodium and potassium concentrations under thermal exposure in Tharparkar and Karan Fries heifers. Biological Rhythm Research 42: 131–39.
Belibasakis N G and Tsirgogianni D. 1996. Effects of niacin on milk yield, milk composition and blood components of dairy cows in hot weather. Animal Feed Science and Technology 64: 53–59.
Bhooshan N, Kumar P and Yadav M C. 2010. Changes in plasma metabolites, enzymes and minerals from birth to sexual maturity in goats. Indian Journal of Animal Sciences 80: 422– 27.
Campbell J M, Murphy M R, Christensen R A and Overton T R. 1994. Kinetics of niacin supplements in lactating dairy cows. Journal of Dairy Science 77: 566–75.
Collier R J, Dahl G E and Van Baale M J. 2006. Major advances associated with environmental effects on dairy cattle. Journal of Dairy Science 89: 1244–53.
Das S K, Upadhyaya R C and Madan M L. 1999. Heat stress in Murrah buffalo calves. Livestock Production Science 61: 71– 78.
Dhali A, Mehla R K and Sirohi S K. 2005. Effect of urea supplemented and urea treated straw based diet on milk urea concentration in crossbred Karan Fries cows. Italian Journal of Animal Science 4: 25–34.
Di Costanzo A, Spain J N and Spiers D E. 1997. Supplementation of nicotinic acid for lactating Holstein cows under heat stress conditions. Journal of Dairy Science 80: 1200–06.
Erickson P S, Murphy M R and Clark J K. 1992. Supplementation of dairy cow diets with calcium salts of long-chain fatty acids and nicotinic acid in early lactation. Journal of Dairy Science 75: 1078–89.
Ganie A H. 2012. Immunological and biochemical changes during thermal stress in buffaloes supplemented with vitamin C. Thesis submitted to National Dairy Research Institute, Karnal, Haryana.
Johnson H D, Ragsdale A C, Berry I L and Shanklin M D. 1963. Temperature-humidity effects including influence of acclimation in feed and water consumption of Holstein cattle. Missouri Agricultural Experiment Station Research Bulletin No. 846.
Kadzere C T, Murphy M R, Silanikove N and Maltz E. 2002. Heat stress in lactating dairy cows: a review. Livestock Production Science 77: 59–91.
Khan N, Kewalramani N and Kant K. 2012. Niacin supplementation for lactating cows under heat stress conditions - A review. Agricultural Reviews 33(2): 143–49.
Kumar B V S, Singh G and Meur S K. 2010. Effect of addition of electrolyte and ascorbic acid in feed during heat stress in buffaloes. Asian Australasian Journal of Animal Sciences 23: 880–88.
Kumar R and Dass R S. 2006. Effect of niacin supplementation on growth, nutrient utilization and blood biochemical profile in male buffalo calves. Asian Australasian Journal of Animal Sciences 19: 1422–28.
Kumar S B V, Ajeet K and Meena K. 2011. Effect of heat stress in tropical livestock and different strategies for its amelioration. Journal of Stress Physiology and Biochemistry 7: 45–54.
Manish K, Jindal R and Nayyar S. 2011. Influence of heat stress on antioxidant status in Beetal goats. Indian Journal of Small Ruminants 17: 178–81.
Marai I F M and Habeeb A A M. 2010. Buffalo’s biological functions as affected by heat stress - a review. Livestock Science 127: 89–109.
Minor D J, Trower S L, Strang B D, Shaver R D and Grummer R R. 1998. Effects of non-fiber carbohydrate and niacin on periparturient metabolic status and lactation of dairy cows. Journal of Dairy Science 81: 189–200.
More A D, Deshpnade S D and Jinke M R. 2008. Serum glucose, total lipid and total cholesterol levels in yearling Osmanabadi goats. Indian Journal of Small Ruminants 14: 110–13.
Nichoff I D, Huther L and Lebzien P. 2009. Niacin for dairy cattle: a review. British Journal of Nutrition 101: 5–19.
NRC. 2001. Nutrient requirements of dairy cattle. seventh revised ed. National Academy press, Washington, DC.
Sharma N, Singh N K, Singh O P, Panday V and Verma P K. 2011. Oxidative stress and antioxidant status during transition period in dairy cows. Asian Australasian Journal of Animal Sciences 24: 479–84.
Shingu H, Hodate K, Kushibiki S, Touno E, Oshibe A, Ueda Y, Shinoda M and Ohashi S. 2009. Effects of growth hormonereleasing hormone treatment on milk production and plasma hormones and metabolites in lactating Japanese Black cows under negative energy balance. Journal of Animal Science 87: 1247–53.
Shipe W F, Senyk G F and Fountain K B. 1980. Modified copper soap solvent extraction method for measuring free fatty acids in milk. Journal of Dairy Science 63: 193–98.
Silanikove N. 2000. Effect of heat stress on the welfare of extensively managed domestic ruminants. Livestock Production Science 67: 1–18.
Small D J V. 2010. Effect of feeding supplemental rumenprotected niacin (NiashureTM) on milk yield and milk composition in early lactation Holstein cows. M.Sc. thesis submitted to the graduate faculty of North Carolina state university.
Snedecor G W and Cochran W G. 1989. Statistical methods. 8th ed. Lowa State University Press, Ames, lowa, USA.
Soly M J and Singh S V. 2001. Physiological and haematological responses of crossbred males under different housing conditions. Thesis submitted to faculty of National Dairy Research Institute, Karnal, Haryana.
SPSS Inc., 1999. SPSS Base 10. Statistical package for windows. Chicago, IL, USA.
St Pierre N R, Cobanov B and Schnitkey G. 2003. Economic losses from heat stress by US livestock industries. Journal of Dairy Science 80: 86–92.
Upadhyay R C, Singh S V, Kumar A, Gupta S K and Ashutosh. 2007. Impact of climate change on impact of Murrah buffaloes. Indian Journal of Animal Sciences 6: 1329–32.
Weiss W P and Gonzalo F. 2006. Are your cows getting the vitamin they need? WCDS Advances in Dairy Technology 18: 249– 59.
West J W. 2003. Effects of heat-stress on production in dairy cattle. Journal of Dairy Science 86: 2131–44.
Wise A, Foord S M and Fraser N J. 2003. Molecular identification of high and low affinity receptors for nicotinic acid. Journal of Biological Chemistry 278: 9869–74.
Wrinkle S R, Robinson P H and Garrett J E. 2012. Niacin delivery to the intestinal absorptive site impacts heat stress and productivity responses of high producing dairy cows during hot conditions. Animal Feed Science and Technology 175: 33– 47.
Yuan K, Shaver R D, Espineria M and Bertics S J. 2011. Effect of a rumen-protected niacin product on lactation performance by dairy cows during summer in Wisconsin. Professional Animal Scientist 27: 190–94.
Zimbelman R B, Baumgard L H and Collier R J. 2010. Effects of encapsulated niacin on evaporative heat loss and body temperature in moderately heat stressed lactating Holstein cows. Journal of Dairy Science 93: 2387.
Zimbelman R B, Collier R J and Bilby T R. 2008. Effect of feeding rumen-protected niacin on core body temperature and milk production in lactating Holstein dairy cows during summer heat stress. Journal of Dairy Science 91(E-suppl. 1): 142.
Zimbelman R B, Collier R J and Bilby T R. 2013. Effect of utilizing rumen protected niacin on core body temperature as well as milk production and composition in lactating dairy cows during heat stress. Animal Feed Science and Technology 180: 26–33.
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