Influence of different culture media on embryo development following in vitro fertilization of caprine oocytes
DOI:
https://doi.org/10.56093/ijans.v88i1.79500Keywords:
Caprine oocytes, Cleavage, In vitro fertilization, In vitro maturation, MorulaAbstract
The present study was designed to compare the developmental competence of in vitro fertilized caprine embryos at different cell stages in three different culture medium(TCM-199, mSOF, KSOM). Oocytes were collected from ovaries and matured in TCM-199 medium containing FSH (5 μg/ml), LH (5 μg/ml) and estradiol-17β (1 μg/ml), supplemented with 20% fetal bovine serum at 38.5 °C and 5% CO2 in an incubator under humidified air for 27 h. After 27 h of in vitro maturation (IVM), oocytes were denuded, washed and co-incubated with 50 μl neat semen diluted in 5 ml of sperm TALP containing 20% EGS and 10μg/ml heparin. Oocytes-sperm after 18h of co-incubation were randomly divided into three groups: Group1(TCM-199 containing 10% NCS and 4mg/ml bovine serum albumin), Group 2(Synthetic oviductal fluid containing 10% NCS and 4mg/ml BSA) and Group 3(Potassium simplex optimization medium (KSOM) containing 10% NCS and 4mg/ml BSA) and found the cleavage rate, morulae and blastocyst percentage for groups 1, 2, and 3 were 13.6, 10.14 and 2.41%, 11.7, 7.6 and 3.84% and 31.4, 12.73 and 6.05%, respectively. Statistical analysis revealed that the cleavage rate and embryo development up to morula and blastocyst stage in group 3(KSOM) was significantly higher (P<0.01%) as compare to group1 and group2.
References
Arias E Maria, Ross J Pablo and Felmer N Ricardo. 2013. Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos. Biological Research 46: 452–62.
Camargo L S A, Viana J H M, S A W F Ferreira, A M Ramos and Filho Vale V R. 2006. Factors influencing in vitro embryo production. Animal Reproduction 3: 19–28.
Garcia Moreno Diego, Nera Alberto, Briand Lamia, Topie Emmanuel, Bencharif Djemil and Tainturier Daniel. 2016. Recent advances on in vitro fertilization. Chapter 2. In Vitro Fertilization (IVF). ISBN 978–93–86337–13–9. Avid Science Publishers.
Herrick J R, Behboodi E, Memili E, Blash S, Echelard Y and Krisher R L. 2004. Effect of macromolecule supplementation during in vitro maturation of goat oocytes on developmental potential. Molecular Reproduction and Development 69: 338– 46.
Karami S H and Akhondi M. 2012. Effect of glucose levels and MEM vitamins during the first step of IVC in synthetic oviduct fluid medium on in vitro development of ovine zygotes. Small Ruminent Research 106: 54–58.
Khan F A, Bhat M H, Yaqoob S H, Waheed S M, Naykoo N A, Athar H, Khan H M, Fazili M R, Ganai N A, Singla S K and Shah R A. 2014. In vitro development of goat-sheep and goatgoat zona-free cloned embryos in different culture media. Theriogenology 81(3): 419–23.
Kharche S D, Goel A K, Jindal S K, Sinha N K and Yadav P. 2008. Effect of somatic cells co-cuture on cleavage and development of in vitro fertilized caprine embryos. Indian Journal of Animal Sciences 78(7): 686–92.
Kharche S D, Goel A K, Jindal S K, Goel P and Jha B K. 2011. Birth of twin kids following transfer of in-vitro produced goat embryos. Indian Journal of Animal Sciences 81(11): 1132– 34.
Kocyigit Alper. 2016. A review of in vitro culture systems in bovine reproductive biotechnologies. Journal of Veterinary Research and Animal Husbandry 1(1): 101.
Lekola Molvia Podile Khomotso. 2015. Improvement of cattle oocyte retrieval techniques and hormonal influence on in vitro embryonic development. A mini-dissertation submitted in partial fulfillment of the requirements for the degree of Master in science Agriculture University of Limpopo, South Africa.
Menchaca A, Barrera N, Dos PC Santos Neto, Cuadro F and Crispo M. 2016. Advances and limitations of in vitro embryo production in sheep and goats. Animal Reproduction 13(3): 273–78.
Neira J A, Tenturier D, Pena M A and Martal J. 2010. Effect of asscosiation of IGF, IGF–2, bFGF, TGF-B1, GM-CSF and LIF on the development of bovine embryos produced in vitro. Theruogenology 3: 95–604.
Shabankareh H K and Akhondi M. 2012. Effect of glucose levels and MEM vitamins during the first step of IVC in synthetic oviduct fluid medium on in vitro development of ovine zygotes. Small Ruminant Research 106: 54–58.
Snedecor G W and Cochran W G. 1989. Statistical Methods, 6th edition. The lowa State University Press, USA.
Wan YongJie, Zhang Yanli, Zhou Zhengrong, Jia Ruoxin, Meng LI, Huang Rong, Wang Ziyu, Yan Yibo, You Jihao, Zhang Guomin and Wang Feng. 2013. Effect of potassium simplex optimization medium KSOM and embryo screening on the production of human lactoferrin transgenic cloned dairy goats. African Journal Of Biotechnology 12(49): 6887–93.
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