Association between FTO gene polymorphism and productivity traits in Lithuanian pigs population


Abstract views: 147 / PDF downloads: 40

Authors

  • R MIÐEIKIENË Researcher, Institute of Biology Systems and Genetic Research, Kaunas, Lithuania
  • I PAKAÐIÛTË Master Student, Institute of Biology Systems and Genetic Research, Kaunas, Lithuania
  • R BIÞIENË Researcher, Institute of Biology Systems and Genetic Research, Kaunas, Lithuania
  • N PEÈIULAITIENË Professor, Institute of Biology Systems and Genetic Research, Kaunas, Lithuania
  • I MICEIKIENË Associate Professor, Institute of Biology Systems and Genetic Research, Kaunas, Lithuania
  • N MAKÐTUTIENË Associate Professor, Institute of Biology Systems and Genetic Research, Kaunas, Lithuania
  • K MORKÛNIENË Lecturer, Institute of Biology Systems and Genetic Research, Kaunas, Lithuania

https://doi.org/10.56093/ijans.v88i1.79511

Keywords:

Carcass traits, FTO gene, Lithuanian pigs, Productivity traits, Polymorphism, SNP

Abstract

FTO in pigs have reported associations of several single nucleotide polymorphisms with some fat-related traits. The purpose of our study was to investigate the FTO gene single nucleotide polymorphism (SNP) (g.400C>G) in the population of pigs and to evaluate the influence of polymorphism on productivity traits. This study of porcine FTO gene g.400C>G SNP was established from the isolated genomic DNA, amplified by nested polymerase chain reaction (PCR) and digested with restriction enzymes, then DNA fragments were separated by agarose gel electrophoresis. Allele C observed with frequency 0.4, allele G – 0.6. The most common genotype was GG, genotype CC was the rarest. CC genotype pigs consume the most feed per kilogram of weight gain compared with other genotypes. The highest values for backfat thickness at the last vertebra at Fat1 and Fat2 were observed in animals with genotype CC. The lowest muscularity (%) was also observed in CC genotype. It was found that almost all pig production traits were significantly influenced by breed. The breed and genotype interaction influence was statistically significant for muscularity of the carcasses and backfat at the Fat2 thickness.

Downloads

Download data is not yet available.

References

Balatsky V, Bankovska I, Pena R N, Saienko A, Buslyk T, Korinnyi S and Doran O. 2016. Polymorphisms of the porcine cathepsins, growth hormone-releasing hormone and leptin receptor genes and their association with meat quality traits in Ukrainian Large White breed. Molecular Biology Reports 43: 517. DOI: https://doi.org/10.1007/s11033-016-3977-z

Braglia S, Zappaterra M, Zambonelli P, Comella M, Dall’Olio S and Davoli R. 2014. Analysis of g.265T > C SNP of fatty acid synthase gene and expression study in skeletal muscle and backfat tissues of Italian Large White and Italian Duroc pigs. Livestock Science 162: 16–18. DOI: https://doi.org/10.1016/j.livsci.2014.01.014

Dvoøáková V, Bartenschlager H, Stratil A, Horák P, Stupka R, Cítek J, Sprysl M, Hrdlicová A and Geldermann H. 2012. Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses. Genetics Selection Evolution 44: 1–13. DOI: https://doi.org/10.1186/1297-9686-44-13

Fan B, Du Z Q and Rothschild M F. 2009. The fat mass and obesity-associated (FTO) gene is associated with intramuscular fat content and growth rate in the pig. Animal Biotechnology 20(2):58–70. DOI: https://doi.org/10.1080/10495390902800792

Fan B, Lkhagvadorj S, Cai W, Young J, Smith R M, Dekkers J C, Huff-Lonerga E, Lonergan S M and Rothschild M F. 2010. Identification of genetic markers associated with residual feed intake and meat quality traits in the pig. Meat Science 84: 645–50. DOI: https://doi.org/10.1016/j.meatsci.2009.10.025

Fontanesi L and Russo V. 2013. Nucleotide variability and haplotype heterogeneity at the porcine fat mass and obesityassociated (FTO) gene. Animal Genetics 44(1): 96–100. DOI: https://doi.org/10.1111/j.1365-2052.2012.02352.x

Fontanesi L, Scotti E, Buttazzoni L, Dall’Olio S, Bagnato A, Lo Fiego D P, Davoli R and Russo V. 2010. Confirmed association between a single nucleotide polymorphism in the FTO gene and obesity-related traits in heavy pigs. Molecular Biology Reports 37(1): 461–66. DOI: https://doi.org/10.1007/s11033-009-9638-8

Fu Y, Li L and Ren S. 2013. Effect of FTO expression and polymorphism on fat deposition in suzhong pigs. Asian Australasian Journal of Animal Sciences 26(10): 1365–73. DOI: https://doi.org/10.5713/ajas.2013.13055

Jacyno E, Pietruszka A, Kawecka M, Biel W and Kolodziej- Skalska A. 2015. Phenotypic correlations of backfat thickness with meatiness traits, intramuscular fat, longissimus muscle cholesterol and fatty acid composition in pigs. South African Journal of Animal Science 45 (2): 122–28. DOI: https://doi.org/10.4314/sajas.v45i2.2

JevsinekSkok D, Kune T, Kovac M, Malovrh S, Potocnik K, Petric N, Zgur S, Dovc P And Horvat A. 2016. FTO gene variants are associated with growth and carcass traits in cattle. Animal Genetics 47(2): 219– 22. DOI: https://doi.org/10.1111/age.12403

Madsen M B, Birck M M, Fredholm M and Cirera S. 2010. Expression studies of the obesity candidate gene FTO in pig. Animal Biotechnology 21(1): 51–63. DOI: https://doi.org/10.1080/10495390903381792

Moravèíková N, Buèko O and Trakovická A. 2014. Effect of the fat mass and obesity associated (FTO) gene polymorphism on carcass traits in pigs. Acta Agraria Kaposváriensis 18: 141– 45.

Peakall R and Smouse P E. 2006. Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–95. DOI: https://doi.org/10.1111/j.1471-8286.2005.01155.x

Sebert S, Salonurmi T, Keinänen-Kiukaanniemi S, Savolainen M, Herzig K H, Symonds M E and Järvelin M R. 2014. Programming effects of FTO in the development of obesity. Acta Physiologica 210: 58–69. DOI: https://doi.org/10.1111/apha.12196

Szydlowski M, Salamon S, Grzes M and Switonski M. 2012. SNP in the 5' flanking region of the pig FTO gene is associated with fatness in Polish Landrace. Livestock Science 150: 397– 400. DOI: https://doi.org/10.1016/j.livsci.2012.09.001

Tung Y C and Yeo G S. 2011. From GWAS to biology: lessons from FTO. Annals of the New York Academy of Sciences 1220: 162–71. DOI: https://doi.org/10.1111/j.1749-6632.2010.05903.x

Tao X, Men X M, Deng B and Xu Z W. 2013. Effects of breed, postnatal development, and nutrition on mRNA expression of the FTO gene in porcine muscle and its relationship with intramuscular fat deposition. Czech Journal of Animal Science 8: 381–88. DOI: https://doi.org/10.17221/6905-CJAS

Zhang B, Zhang Y, Zhang L, Wang J, Li Z and Chen H. 2011.Allelic polymorphism detected in the bovine FTO gene. Molecular Biotechnology 49(3): 257–62. DOI: https://doi.org/10.1007/s12033-011-9400-z

Downloads

Submitted

2018-05-07

Published

2018-05-07

Issue

Section

Articles

How to Cite

MIÐEIKIENË, R., PAKAÐIÛTË, I., BIÞIENË, R., PEÈIULAITIENË, N., MICEIKIENË, I., MAKÐTUTIENË, N., & MORKÛNIENË, K. (2018). Association between FTO gene polymorphism and productivity traits in Lithuanian pigs population. The Indian Journal of Animal Sciences, 88(1), 86-90. https://doi.org/10.56093/ijans.v88i1.79511
Citation