Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR

P CHOORI; S S PATIL; D RATNAMMA; APRAJAPATI; S Y MUKARTAL; G B MANJUNATHA REDDY; K P SURESH; D HEMADRI; H RAHMAN

doi:10.56093/ijans.v87i12.79743

Authors

P CHOORI ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
S S PATIL ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
D RATNAMMA ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
APRAJAPATI ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
S Y MUKARTAL ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
G B MANJUNATHA REDDY ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
K P SURESH ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
D HEMADRI ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India
H RAHMAN ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 India

https://doi.org/10.56093/ijans.v87i12.79743

Keywords:

Antigen, Antibody, Classical swine fever, ELISA, Karnataka, RT-PCR, Seroprevalence

Abstract

Classical swine fever is highly contagious viral disease of swine causing huge economic losses to pig farmers
and pig industry. The study was conducted in Karnataka to understand the current scenario of CSF in the state and
to evaluate efficiency of diagnostic tests (antigen ELISA and RT-PCR) in detecting the disease. Serum samples
were collected from 270 pigs from 13 districts of the state and were tested for the presence of CSF antibodies.
Whole blood samples (151) from 14 outbreaks of CSF were collected for the comparative diagnosis of the disease
using Antigen ELISA and reverse transcriptase (RT)-PCR. A seroprevalence of 28.5% (77/270) was found in the
serum samples collected form the whole Karnataka. The southern Karnataka had higher seroprevalence (47%) in
comparison to northern Karnataka region where seroprevalence was 17%. It confirms endemicity of the disease in
southern region. Of 151 blood samples collected for comparative diagnosis, 61 samples were positive for CSF by
RT-PCR and 39 by antigen ELISA, indicating the superiority of RT-PCR over antigen ELISA to detect CSFV
infection in earliest stages of infection.

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