Molecular diagnosis and antibiogram of Pasteurella multocida type B associated with septicemic pasteurellosis in pigs

MENAKA SETHI; SANTOSH KUMAR; NEELAM TOMAR; JENY K JOHN; TARENI DAS; R K AGARWAL; G SAIKUMAR

doi:10.56093/ijans.v87i12.79831

Authors

MENAKA SETHI ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
SANTOSH KUMAR ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
NEELAM TOMAR ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
JENY K JOHN ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
TARENI DAS ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
R K AGARWAL ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
G SAIKUMAR ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v87i12.79831

Keywords:

Antibiogram, Molecular diagnosis, Multiplex PCR, Pasturella multocida type B, Pigs, Septicemia

Downloads

Download data is not yet available.

References

1. Cardoso-Toset F, Gómez-Laguna J, Callejo M, Vela A I, Carrasco L, Fernández-Garayzábal J F, Maldonado A and Luque I. 2013. Septicaemic pasteurellosis in free range pigs associated with an unusual biovar 13 of Pasteurella multocida. Veterinary Microbiology 167(3–4): 690–94. DOI: https://doi.org/10.1016/j.vetmic.2013.08.005

2. Carter G R. 1955. Studies on Pasteurella multocida I. A haemoagglutination test for the identification of serological types. American Journal of Veterinary Research 16(60): 481– 84.

3. García N, Fernández-Garayzábal J F, Goyach J, Domínguez L and Vela A I. 2011. Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain. Veterinary Record 169(14): 362. DOI: https://doi.org/10.1136/vr.d4869

4. Ghosh R C, Hirpurkar S D and Mondal M. 2011. An outbreak of swine pasteurellosis in Chhattisgarh. Indian Journal of Veterinary Pathology 35 (1): 87-88.

5. Hansen M S, Pors S E, Jensen H E, Hansen V B, Bisgaard M, Flachs F M and Nielsen O L. 2010. An investigation of the pathology and pathogens associated with porcine respiratory disease complex in Denmark. Journal of Comparative Pathology 143(2–3): 120–31. DOI: https://doi.org/10.1016/j.jcpa.2010.01.012

6. John J K, Das T, Kumar S, Sethi M, Vamadeavan B, Tomar N and Saikumar G. 2017. Polyserositis associated with Pasturella multocida Type A in a crossbred pig. International Journal of Livestock Research 7(4): 268-74. DOI: https://doi.org/10.5455/ijlr.20170312053347

7. Kalorey D R, Yuvaraj S, Vanjari S S, Gunjal P S, Dhanawade N B, Barbuddhe S B and Bhandarkar A G. 2008. PCR analysis of Pasteurella multocida isolates from an outbreak of pasteurellosis in Indian pigs. Comparative Immunology, Microbiology and Infectious Diseases 31(6): 459–65. DOI: https://doi.org/10.1016/j.cimid.2007.06.003

8. Kumar H, Sharma S, Mahajan V, Verma S, Arora A K, Kaur P and Sandhu K S. 2006. Pathology and PCR based confirmation of haemorrhagic septicemia outbreaks in bovines. Indian Journal of Veterinary Pathology 30(1): 5–8.

9. Kumar H, MahajanV, Sharma S, Alka, Singh R, Arora A K, Banga H S, Verma S, Kaur K, Kaur P, Meenakshi and Sandhu K S. 2007. Concurrent pasteurellosis and classical swine fever in Indian pigs. Journal of Swine Health and Production 15(5): 279–83.

10. OIE. 2008. Terrestrial manual. Haemorrhagic septicaemia. pp. 739–51.

11. Portis E, Lindeman C, Johansen L and Stoltman G. 2012. A ten- year (2000–2009) study of antimicrobial susceptibility of bacteria that cause bovine respiratory disease complex— Mannheimia haemolytica, Pasturella multocida and Histophil somni in the United States and Canada. Journal of Veterinary Diagnostic Investigation 24(5): 932–44. DOI: https://doi.org/10.1177/1040638712457559

12. Ray P K, Desingu P A, Sethi M, Nagaleekar V K, Singh R and Saikumar G. 2016. Pathological investigation of an outbreak of pneumonic Pasteurellosis in pigs. Indian Journal of Veterinary Pathology 40(1): 58–62. DOI: https://doi.org/10.5958/0973-970X.2016.00011.0

13. Register K B, Brockmeier S L, Jong M F and Pijoan C. 2012. Pasteurellosis. Diseases of Swine. 10th edn, pp.798-810. (Eds) Zimmerman J J, Karriker L A, Ramirez A, Schwartz K J and Stevenson G W. Wiley-Blackwell, Ames, Iowa.

14. Sarangi L N, Priyadarshini A, Kumar S, Thomas P, Gupta S K, Nagaleekar V K and Singh V P. 2014. Virulence genotyping of Pasteurella multocida isolated from multiple hosts from India. Scientific World Article ID 814109, 10 pages. DOI: https://doi.org/10.1155/2014/814109

15. Sellyei B, Varga Z, Szentesi-samu K, Kaszanyitzky E and Magyar T. 2009. Antimicrobial susceptibility of Pasteurella multocida isolated from swine and poultry. Acta Veterinaria Hungarica 57(3): 357–67. DOI: https://doi.org/10.1556/avet.57.2009.3.2

16. Shivachandra S B, Viswas K N and Kumar A A. 2011. A review of hemorrhagic septicemia in cattle and buffalo. Animal Health Research Reviews 12(1): 67-82. DOI: https://doi.org/10.1017/S146625231100003X

17. Sorensen V, Jorsal S E and Mousing J. 2006. Diseases of the respiratory system. Diseases of Swine, 9th edn, pp 149–177. (Eds) Straw B E, Zimmerman J J, D’Allarie S and Taylor D J. Blackwell Publishing, Ames, Iowa.

18. Tigga M, Ghosh R C, Malik P, Choudhary B K, Tigga P and Nagar D K. 2014. Isolation, characterization, antibiogram and pathology of Pasteurella multocida isolated from pigs. Veterinary World 7(5): 363–68. DOI: https://doi.org/10.14202/vetworld.2014.363-368

19. Townsend K M, O’Boyle D, Phan T T, Xuan H T, Wijewardana T G, Wilkie I, Trung N T and Frost A J. 1998. Acute septicaemic pasteurellosis in Vietnamese pig. Veterinary Microbiology 63(2–4): 205–15. DOI: https://doi.org/10.1016/S0378-1135(98)00233-8

20. Ujvári B, Szeredi L, Pertl L, Tóth G, Erdélyi K, Jánosi S, Molnár T and Magyar T. 2015. First detection of Pasteurella multocida type B:2 in Hungary associated with systemic pasteurellosis in backyard pigs. Acta Veterinaria Hungarica 63(2): 141–56. DOI: https://doi.org/10.1556/avet.2015.012

21. Varte Z, Dutta T K, Roychoudhury P, Begum J and Chandra R. 2014. Isolation, identification, characterization and antibiogram of Pasteurella multocida isolated from pigs in Mizoram with special reference to progressive atrophic rhinitis. Veterinary World 7(2): 95–99. DOI: https://doi.org/10.14202/vetworld.2014.95-99

22. Verma H, Rawat M, Samal A and Upmanyu V. 2014. Conventional and molecular characterization of Pasteurella multocida isolated from a case of swine septicaemic pasteurellosis. Indian Journal of Animal Research 48(6): 605–08. DOI: https://doi.org/10.5958/0976-0555.2014.00040.5