Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle

M V CHAUDHARI; I D GUPTA; ARCHANA VERMA; AVTAR SINGH; RAMENDRA DAS; L SAILO; S V SINGH

doi:10.56093/ijans.v87i12.79869

Authors

M V CHAUDHARI ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
I D GUPTA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
ARCHANA VERMA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
AVTAR SINGH ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
RAMENDRA DAS ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
L SAILO ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
S V SINGH ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v87i12.79869

Keywords:

Age at calving, Bos indicus, Gene substitution model, HSPB1 gene, SNP

Abstract

The study was planned with objective to screen single nucleotide polymorphisms (SNPs) in bovine HSPB1 gene and to find its effect on age at calving in Karan-Fries (Bos taurus × Bos indicus) and Sahiwal (Bos indicus) breeds of cattle. Genomic DNA was extracted from whole blood of 180 cows of both breeds. Based on publically available bovine HSPB1 gene sequence, one primer set was used for polymerase chain reaction amplification of the target region. Further DNA sequencing revealed a transition of thymine to cytosine at SNP rs208395876 in 5-UTR and a silent transversion of guanine to thymine at SNP rs723061520 in first coding sequence of bovine HSPB1 gene in both the studied breeds. Effect of individual SNP genotypes of bovine HSPB1 gene with age at calving (months) was analyzed separately in both breeds via regression using a repeated gene substitution MIXED model and least-squares means.The overall observed heterozygosity in both breeds and the F-Statistics values indicated that there was lesser genetic diversity in studied genomic region of bovine HSPB1 gene in Bos indicus compared to crossbred cattle. Association analysis revealed that SNP rs208395876 significantly delayed age at calving in Karan-Fries cows. In conclusion, the studied genomic region of bovine HSPB1 gene is polymorphic. In addition, these polymorphisms were informative with regard to age at calving of crossbred cows. Therefore, this gene is an important candidate for cow fertility.

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