Evidence of ovine brucellosis due to Brucella ovis and Brucella melitensis in Karnataka, India

R SHOME; S SAHAY; K TRIVENI; N KRITHIGA; B R SHOME; H RAHMAN

doi:10.56093/ijans.v88i5.79934

Authors

R SHOME Principal Scientist, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
S SAHAY Research Fellow, Bacteriology Lab-1, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
K TRIVENI Research Fellow, Bacteriology Lab-1, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
N KRITHIGA Research Fellow, Bacteriology Lab-1, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
B R SHOME Principal Scientist, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
H RAHMAN Regional Representative for South Asia, ILRI

https://doi.org/10.56093/ijans.v88i5.79934

Keywords:

Brucella ovis, Brucella melitensis, iELISA, India, PCR, Random sampling, RBPT

Abstract

Ovine brucellosis is often neglected contagious bacterial disease causing enormous economic losses to sheep industry. India is recognized as geographical hotspot for brucellosis and there is only one seroprevalence report of Brucella ovis infection in sheep. Ovine brucellosis is caused by both rough and smooth strains of Brucella sp. In the present study, 300 sheep serum samples collected from 9 different districts of Karnataka by 3 stage random sampling approach were screened by antibody and DNA detection tests (RBPT and iELISA) and PCR. Antibodies specific to B. melitensis and B. ovis infection were 8.67% and 5.34%, respectively. In the same set of samples, 5% (15/300) showed Brucella DNA amplification by bcsp31 and IS711 genus specific PCRs. In B. melitensis and B. ovis species specific PCRs, 3.34% and 1.67%, respectively were detected positive indicating presence of both Brucella species in sheep population. Among the 7 districts, Yadgir, Tumkur, Raichur and Bagalkot showed higher prevalence of antibodies against rough and smooth strains of Brucella. This emphasizes the need for sensitization of national system in designing surveillance and control strategies for both B. ovis and B. melitensis infections in sheep.

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References

Adone R and Pasquali P. 2013. Epidemiosurveillance of brucellosis. Revue Scientifique ET Technique Office International Epizootics 32: 199–205. DOI: https://doi.org/10.20506/rst.32.1.2202

Alton G G, Jones L M, Angus R D and Verger J M. 1988. Techniques for the Brucellosis Laboratory. National Institute of Research, Nouzilly, France.

Arsenault J, Girard C, Dubreuil P and Bélanger D. 2004. Lack of evidence of Brucella ovis infection in rams in Quebec. Canadian Veterinary Journal 45: 312–14.

Baily G C, Kraahn J B, Drasar B S and Stokeer N G. 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. Journal of Tropical Medicine and Hygiene 95: 271–75.

Bricker B J and Halling S M. 1994. Differentiation of Brucella abortus bv 1, 2 and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv 1 by PCR. Journal of Clinical Microbiology 32: 2660–66. DOI: https://doi.org/10.1128/jcm.32.11.2660-2666.1994

Chand P, Sadana J R and Malhorta A K. 2002. Epididymo-orchitis caused by Brucella melitensis in breeding rams in India. Veterinary Record 15: 84–85. DOI: https://doi.org/10.1136/vr.150.3.84

Chávez J M, Cháirez F G E, Flores C F A, Alenzuela R B and Pérez J L T. 2013. Possible risk factors for serological prevalence of Brucella ovis in Zacatecas, Mexico. Revista Mexicana de Ciencias Pecuarias 4: 61–74.

Gall D, Nielsen K, Vigliocco A, Smith P, Perez B, Rojas X and Robles C. 2003. Evaluation of an indirect enzyme-linked immunoassay for presumptive serodiagnosis of Brucella ovis in sheep. Small Ruminant Research 48: 173–79. DOI: https://doi.org/10.1016/S0921-4488(03)00013-0

Gupta V K, Verma D K, Singh S V and Vihan V S. 2007. Serological diagnostic potential of recombinant outer membrane protein (Omp31) from Brucella melitensis in goat and sheep brucellosis. Small Ruminant Research 70: 260–66. DOI: https://doi.org/10.1016/j.smallrumres.2006.01.012

Gupta V K, Jyoti V, Ranjeeta K and Vihan V S. 2009. Evaluation of an ELISA using recombinant outer membrane protein 31 for diagnosis of ovine brucellosis. Indian Journal of Animal Sciences 79: 41–43.

Habtamu T T, Rathore R, Dhama K and Karthik K. 2013. Isolation and molecular detection of Brucella melitensis from disease outbreak in sheep and B. abortus from cattle farm by IS711 and omp2a gene based PCR. International Journal of Current Research 5: 1920–25.

Hinic V, Brodard I and Thomann A. 2008. Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis and B. neotomae suitable for both conventional and real-time PCR systems. Journal of Microbiological Methods 75: 375–78. DOI: https://doi.org/10.1016/j.mimet.2008.07.002

Katoch R C, Joshi V B, Sharma M, Batta M K and Nagal K B. 1996. Seroprevalence of Brucella ovis, Brucella melitensis and Chlamydia psittaci in rams. Indian Journal of Animal Sciences 66: 1130–31.

Maninder S, Singh D K, Shivaramu K V, Biswas R, Rawat S, Boral R, Singh S and Cheema P S. 2010. Serum as clinical specimen in PCR for diagnosis of ovine brucellosis. Indian Journal of Animal Sciences 80: 17–18.

Manterola L, Tejero-Garcés, Ficapal A, Shopayeva G, Blasco G, Marin J M and López-Goñi C M. 2003. Evaluation of a PCR test for the diagnosis of Brucella ovis infection in semen samples from rams. Veterinary Microbiology 92: 65–72. DOI: https://doi.org/10.1016/S0378-1135(02)00310-3

Méndez N G, Díaz A E, Avarez J F M, Romero F A and Giemes F S. 1999. Epididimitis Ovina: Estudios bacteriológico y serológico. Veterinaria Mexico 30: 329–36.

Niilo L, MacDonald D W, Godkin G F and William Stone M. 1986. Ovine brucellosis in Alberta. Canadian Veterinary Journal 27: 245–49.

Praud A, Champion J L, Corde Y, Drapeau A, Meyer L and Garin- Bastuji B. 2012. Assessment of the diagnostic sensitivity and specificity of an indirect ELISA kit for the diagnosis of Brucella ovis infection in rams. BMC Veterinary Research 8: 68–75. DOI: https://doi.org/10.1186/1746-6148-8-68

Petroviæ M, Špièiæ S, Potkonjak A, Lako B, Kostov M and Cvetniæ Z. 2013. Epizootiological, clinical and pathological characteristics of sheep flocks infected with Brucella ovis in the republic of Serbia. Slovenian Veterinary Research 50: 117–24.

Renukaradhya G J, Isloor S and Rajasekhar M. 2002. Epidemiology, zoonotic aspects, vaccination and control/ eradication of brucellosis in India. Veterinary Microbiology 90: 183–95. DOI: https://doi.org/10.1016/S0378-1135(02)00253-5

Singh S V, Gupta V K and Singh N. 2000. Control of ovine brucellosis in a high performing sheep flock using multiple serological tests. Indian Journal of Animal Sciences 70: 231– 34.

Shome R, Shome B R, Deivanai M, Desai G S, Patil S S, Bhure S K and Prabhudas K. 2007. Seroprevalence of brucellosis in small ruminants. Indian Journal of Comparative Microbiology and Immunology and Infectious Diseases 27: 13–15.

Shome R, Padmashree B S, Krithiga N, Triveni K, Sahay S, Shome B R, Singh P and Rahman H. 2014. Bovine brucellosis in organized farms of India - An assessment of diagnostic assays and risk factors. Advances in Animal and Veterinary Science 2: 557–64. DOI: https://doi.org/10.14737/journal.aavs/2014/2.10.557.564

Sonawane G G, Tripathi S and Dubey S C. 2011. Sero-incidence of brucellosis in small ruminants of semiarid Rajasthan. Indian Journal of Animal Sciences 81: 327–29.

Spiciæ S, Marjanoviæ S, Zdelar-Tuk M and Cvetniæ Z. 2009. First evidence of Brucella ovis infection in Republic of Croatia. Deutsche Tierarztliche Wochenschrift 116: 209–13.

Sulima M and Venkataraman K S. 2010. Economic losses due to Brucella melitensis infection in sheep and goats. Tamil Nadu Journal of Veterinary and Animal Sciences 6: 191–92.