Prevalence of syphaciosis in laboratory rodents with emphasis on species identification, treatment and Th2 cytokine response

S T BINO SUNDAR; T J HARIKRISHNAN; BHASKARAN RAVI LATHA; G SARATH CHANDRA; T M A SENTHIL KUMAR; S RAMESH; M R SRINIVASAN; P AZHAHIANAMBI; S GOMATHINAYAGAM; M RAMAN; K G TIRUMURUGAAN; SERMA SARAVANA PANDIAN

doi:10.56093/ijans.v88i7.81404

Authors

S T BINO SUNDAR Assistant Professor, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
T J HARIKRISHNAN Director of Research, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
BHASKARAN RAVI LATHA Professor and Head, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
G SARATH CHANDRA Professor and Head, Pharmacovigilance Laboratory for Animal Feed and Food Safety, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
T M A SENTHIL KUMAR Professor, Department of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
S RAMESH Professor and Head, Laboratory Animal Medicine Unit, Directorate of Centre for Animal Health Studies;
M R SRINIVASAN Assistant Professor, Department of Veterinary Pharmacology and Toxicology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
P AZHAHIANAMBI Assistant Professor, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
S GOMATHINAYAGAM Professor, Department of Veterinary Parasitology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
M RAMAN Programme Head (Vaccines), Translational Research Platform for Veterinary Biologicals, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
K G TIRUMURUGAAN Professor, Department of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
SERMA SARAVANA PANDIAN Assistant Professor, Department of Animal Husbandry Economics, Madras Veterinary College, Chennai

https://doi.org/10.56093/ijans.v88i7.81404

Keywords:

Ivermectin, Rodents, Syphacia obvelata, Syphaciosis, Th2 cytokine

Abstract

Prevalence of syphaciosis (pinworm infection) in laboratory rats and mice, species identification, chemotherapy with ivermectin and its effect on pinworm infection, and Th2 cytokine response was studied. Using perianal tape test examination, the highest rate of pinworm infection was found in male Wistar rats (69.44%) followed by female Wistar rats (62.50%), male Balb C mice (60.60%), female Swiss Albino mice (51.42%), male Swiss Albino mice (33.33%) and female Balb C mice (32.69%). Wistar rats had maximum infection (65.97%) followed by Balb C mice (46.64%) and Swiss albino mice (42.37%). Overall, males had higher infection (54.45%) compared to females (48.87%). Syphacia obvelata in mice and S. muris in rats were the pinworms identified morphologically and confirmed with polymerase chain reaction and sequencing. No mixed infection was found in rats and mice. Ivermectin (2.5 mg/kg) as oral gavage as well as in drinking water continuously for five days was highly effective in eradication
of pinworms in rats and mice. Down regulation of Th2 cytokines, viz. IL4, IL5 and IL13 was observed after ivermectin treatment in both male and female Wistar rats.

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