Melatonin improves in vitro maturation and subsequent embryo development of caprine oocytes*

SURBHI AGARWAL; S D KHARCHE; A K BHATIYA

doi:10.56093/ijans.v88i7.81458

Authors

SURBHI AGARWAL
S D KHARCHE Principal Scientist, Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Mathura, Uttar Pradesh 281 122 India
A K BHATIYA Professor and Head, Department of Biotechnology, GLA University, Mathura, Uttar Pradesh, India

https://doi.org/10.56093/ijans.v88i7.81458

Keywords:

Blastocyst, Caprine, Embryo development, in vitro maturation, Melatonin, Oocytes

Abstract

The aim of the present investigation was to study the effect of melatonin on in vitro maturation and subsequent embryo development of caprine oocytes. In experiment 1, 384 in vitro matured oocytes were selected and randomly divided in to two groups, viz. group 1 (control) wherein oocytes matured in maturation media without supplementation for 27 h in humidified atmosphere at 38.5°C with 5% CO₂ in CO₂ incubator while in group 2 oocytes matured in maturation media with 30 ng/ml melatonin supplementation. After 27 h of culture, nuclear maturation was observed in both groups using Hoechst dye. In experiment 2, 1,336 oocytes were randomly divided into two groups, viz. group 1 (641) wherein oocytes were matured in maturation media without melatonin while in group 2 (n=695) oocytes matured in maturation media with 30 ng/ml melatonin supplementation. After 27 h, oocytes of both groups were then subjected to in vitro fertilization.The rate of nuclear maturation in group 2 (30 ng/ml melatonin) was significantly higher than that of group 1 (control). Similarly, the cleavage rate and blastocyst formation from in vitro matured goat oocytes were significantly higher in group 2 than that of group 1. In conclusion, the result indicated that the supplementation of 30 ng/ml melatonin in maturation media improves the nuclear maturation and subsequent cleavage rates and blastocyst production from caprine oocytes.

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Author Biography

SURBHI AGARWAL

Ph.D Scholar, Department of Biotechnology, GLA University, Mathura, Uttar Pradesh, India

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