Evaluation of immunochromatographic assay as a field test for the diagnosis of caprine brucellosis

B J TRANGADIA; M C PRASAD

doi:10.56093/ijans.v88i8.82908

Authors

B J TRANGADIA Assistant Professor, Department of Veterinary Pathology, Vanbandhu College of Veterinary Science and Animal Husbandry
M C PRASAD Ex-Joint Director (Research), Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh

https://doi.org/10.56093/ijans.v88i8.82908

Keywords:

Brucellosis, Goats, Immunochromatographic assay, Indirect ELISA, RBPT

Abstract

The present study was undertaken to assess the suitability of immunochromatographic assay (ICA) as a field test for the diagnosis of caprine brucellosis. Whole blood samples (570: 195 male goats, 375 female goats) were collected from selected organized herds and panjarapoles located in Southern Gujarat. Out of 570 animals tested, 19 (3.33%), 72 (12.63%) and 45 (7.89%) were positive by Rose Bengal plate test (RBPT), indirect ELISA (iELISA) and immunochromatographic assay (ICA) respectively. The sensitivity of RBPT and ICA vs iELISA was 26.39% and 61.11% respectively. Both the tests were 100% specific when compared with iELISA. Negative likelihood ratio for RBPT and ICA as compared to iELISA was 0.74 and 0.39 respectively. The negative predictive value for RBPT was 90.38% and 94.68% for ICA. However, the positive predictive values (PPV) for both the tests were 100%. McNemar chi-square test for independent data (with Yates’ correction) revealed significant difference in the positive proportion between RBPT vs iELISA as 9.30% and ICA vs iELISA as 4.91%. The concordance of iELISA with RBPT was moderate (k=0.385) while it was good (k=0.733) for ICA.

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