Periparturient rise in the Cryptosporidium oocyst count in Beetal goats and evaluation of infection in new born kids

A KHURSHEED; ANISH YADAV; SHAFIYA IMTIAZ RAFIQI; R KATOCH; R GODARA; S SOOD; T SALEEM

doi:10.56093/ijans.v88i9.83539

Authors

A KHURSHEED PhD Scholar, Sher-e-Kashimir University of Agriculture Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
ANISH YADAV Professor and ICAR National Fellow, Sher-e-Kashimir University of Agriculture Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
SHAFIYA IMTIAZ RAFIQI Research Associate, Sher-e-Kashimir University of Agriculture Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
R KATOCH Professor and Head, Sher-e-Kashimir University of Agriculture Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
R GODARA Assistant Professor, Division of Veterinary Parasitology, Sher-e-Kashimir University of Agriculture Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
S SOOD Associate Professor, Division of Veterinary Pathology, Sher-e-Kashimir University of Agriculture Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
T SALEEM PhD Scholar, Sher-e-Kashimir University of Agriculture Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India

https://doi.org/10.56093/ijans.v88i9.83539

Keywords:

Crytosporodium, Goat, PCR-RFLP, Periparturient rise, Oocyst

Abstract

In cryptosporidial infection, very scarce literature is available about periparturient pattern of oocyst excretion in goats and its implications in the epidemiology of disease in kids; thus the present investigation was done. Faecal samples (160) were examined from 20 pregnant goats, collected at weekly interval, commencing from 4 weeks before kidding up to 3 weeks after kidding. Faecal sample examination by Sheather’s floatation sedimentation technique followed by modified Ziehl-Neelsen staining technique revealed an overall positivity of 26.25% for Cryptosporidium oocysts. Prevalence of oocysts excretion was high around parturition and the number of oocysts shed oscillated between 20–400 oocysts/g (OPG) of faeces. Examination of 60 faecal samples of 20 kids born to studied goats from 1 to 3 weeks of age revealed 40% samples positive for Cryptosporidium oocysts and 65% kids became infected by the end of the 3 weeks. The OPG of kids ranged from 100 to 1,560. Molecular characterization by PCR-RFLP of 18S small subunit (SSU) rRNA gene showed that 73.80% and 26.19% goat samples, and 87.5% and 12.5% kids samples were positive for C. parvum and C. ubiquitum, respectively. The study indicated periparturient transmission of Cryptosporidium spp. from pregnant goats to their new born kids.

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