Genetic variation of highly pathogenic Indian porcine reproductive and respiratory syndrome viruses after introduction in 2013
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https://doi.org/10.56093/ijans.v88i10.84071
Keywords:
GP5, HP-PRRS, India, N protein, ORF5, ORF7, PhylogenyAbstract
To study its possible link to pathogenicity, the genomic variation in full ORF5 and ORF7 genes, and their encoded proteins in 26 field HP-PRRSV isolates from three major HP-PRRS outbreaks occurred in India, since 2013 was analysed. Sequence analysis and phylogenetic tree revealed involvement of genetically different strain in each outbreak of India rather persistence of a single strain. Analysis and comparison of N protein amino acid sequences of HP-PRRSV with VR2332 revealed consistent mutation at position 15D to N or K and 46 K to R in all the HP-PRRSV. GP5 protein showed consistent mutations at 29 positions from that of VR2332. The potential Nglycosylation sites in GP5 was found variable from 4–5 with one additional N-glycan moiety around the neutralizing epitope B. However, the ‘decoy’ epitope A was found highly conserved in all the HP-PRRSV.
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