Superovulatory response with once and twice daily injection of Follitropin-V in crossbred cows

Abstract

Cyclic cows (7) were divided into 2 different treatment groups for superovulation. Group 1 (n=4) cows were treated with follitropin-V (400 mg) subcutaneously diluted in 3.2% gelatin carrier vehicle in descending doses and group 2 (n=3) cows were treated with follitropin-V (400 mg) intramuscularly twice daily in descending doses. All the animals (100%) exhibited superovulatory estrus. The average time required for onset of estrus was 39.5±6.65 h and 27.33±10.53 h and the duration of superovulatory estrus was 27.5±9.16 and 26.5±8.81 h in group 1 and 2, respectively. The average number of corpora lutea and unovulatory follicles were 11±7.58 and 6.33±2.18 and 2.0±0.70 and 2.66±0.66, in group 1 and 2, respectively. The average total number of embryos and transferable embryos in group 1 was 1.75±1.75 and zero, respectively, while in group 2 it was 5±4.51 and 4±3.15, respectively