Molecular epidemiology of rotavirus A in Iranian broiler flocks

HADI HAGHBIN NAZARPAK; HOSSEIN HOSSEINI; ARASH GHALYANCHILANGEROUDI; RIMA MORSHED

doi:10.56093/ijans.v88i12.85749

Authors

HADI HAGHBIN NAZARPAK Assistant Professor, Department of Clinical Sciences, Islamic Azad University, Garmsar, Iran
HOSSEIN HOSSEINI Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
ARASH GHALYANCHILANGEROUDI Associate Professor, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
RIMA MORSHED Associate Professor, Veterinary Group, Iran Encyclopedia Compiling Foundation, Ministry of Science, Research and Technology, Tehran, Iran

https://doi.org/10.56093/ijans.v88i12.85749

Keywords:

Broiler chicken, Iran, Phylogenetic analysis, Rotavirus A

Abstract

Avian rotaviruses (RV) are still largely undefined despite being widespread in several avian species and significant economic the impact of rotavirus enteritis in poultry flocks. In this study, the presence of avian RV groups was investigated in 36 commercial poultry flocks with a history of enteric diseases in Iran. Intestinal contents of broiler chickens with diarrhea and stunting syndrome were analyzed by reverse transcription-polymerase chain reaction specific for RVs based on NSP4 gene. Through partial sequencing and BLAST analyses of 11 positive specimens, we identified avian-like RV group A (RVA) strain. There was high prevalence of group A rotaviruses (approximately 30%) in our samples. The phylogenetic analysis also revealed a close genetic relationship between the current isolates and other avian RVAs but located in the separate cluster. This study provides novel data on the prevalence of genetically different avian RVs in Iranian poultry flocks.

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Author Biographies

HADI HAGHBIN NAZARPAK, Assistant Professor, Department of Clinical Sciences, Islamic Azad University, Garmsar, Iran

Assistant Professor, Department of Clinical Sciences.
HOSSEIN HOSSEINI, Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

Assistant Professor, Department of Clinical
Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran.
ARASH GHALYANCHILANGEROUDI, Associate Professor, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Associate Professor, Department of Microbiology and
Immunology, Faculty of Veterinary Medicine, University of
Tehran, Tehran, Iran
RIMA MORSHED, Associate Professor, Veterinary Group, Iran Encyclopedia Compiling Foundation, Ministry of Science, Research and Technology, Tehran, Iran

Associate Professor, Veterinary Group, Iran Encyclopedia Compiling Foundation, Ministry of Science, Research and Technology, Tehran, Iran.

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