Phylogenetic analysis of Indian isolates of Pasteurella multocida based on partial 16S rRNA gene sequences: Association of caprine isolate with lineage B

MADHU MISHRA; T G SUMITHRA; SANTOSH KUMAR; SANTOSH KUMAR GUPTA; RAJESH KUMAR AGARWAL; VISWAS KONASAGARA NAGALEEKAR

doi:10.56093/ijans.v88i12.85755

Authors

MADHU MISHRA PhD Scholar, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
T G SUMITHRA Scientist, Division of Marine Biotechnology, ICAR-Central Marine Fisheries Research Institute, Cochin, India
SANTOSH KUMAR Research Associate, Division of Pathology, Division of Marine Biotechnology, ICAR-Central Marine Fisheries Research Institute, Cochin, India
SANTOSH KUMAR GUPTA Associate Professor, Faculty of Veterinary and Animal Sciences, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India
RAJESH KUMAR AGARWAL Principal Scientist and Head, Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
VISWAS KONASAGARA NAGALEEKAR Principal Scientist, Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v88i12.85755

Keywords:

Lineage B, Pasteurella multocida, Phylogenetic analysis, 16S rRNA

Abstract

Pasteurella multocida is responsible for diseases, which are endemic and economically important in India, still comparative investigations on phylogenetic relations of Indian P. multocida isolates are scarce. Therefore, present study was undertaken to understand the phylogenetic relationship of several isolates belonging to different host, place of isolation and capsular types based on partial 16S rRNA gene sequencing. In the current study, a 838 bp fragment of 16S rRNA gene of 35 field isolates of P. multocida belonging to different capsular types, recovered from cattle, buffalo, sheep, goat, pigs and birds, collected from different states of India sequenced and analyzed. There were 12 unique 16S rRNA types among 35 isolates, which clustered into 2 distinct phylogenetic lineages, viz. A and B. There were strong correlations between the phylogenetic relations and capsular types, with maximum heterogeneity seen among isolates of capsular type A. However, there was no clustering based on the host or place of isolation indicating the potential hazard of interspecies sharing and the possibility of translocation of infected animals across international borders. Moreover, one of the caprine isolates belonged to lineage B. To the best of our knowledge, this is the first report of a caprine isolate in lineage B, since lineage B is reported to be exclusively associated with birds and cats. It may be alarming that the strains of lineage B are becoming adapted to different host species.

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Author Biographies

MADHU MISHRA, PhD Scholar, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

PhD Scholar,Â ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
T G SUMITHRA, Scientist, Division of Marine Biotechnology, ICAR-Central Marine Fisheries Research Institute, Cochin, India

Scientist, Division of Marine Biotechnology, ICAR-Central Marine Fisheries Research Institute, Cochin, India.
SANTOSH KUMAR, Research Associate, Division of Pathology, Division of Marine Biotechnology, ICAR-Central Marine Fisheries Research Institute, Cochin, India

Research Associate, Division of Pathology.
SANTOSH KUMAR GUPTA, Associate Professor, Faculty of Veterinary and Animal Sciences, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India

Associate Professor, Faculty of Veterinary and Animal
Sciences, Institute of Agricultural Sciences, Banaras Hindu
University, Varanasi, India.
RAJESH KUMAR AGARWAL, Principal Scientist and Head, Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

Principal Scientist and Head, Division of Bacteriology and
Mycology
VISWAS KONASAGARA NAGALEEKAR, Principal Scientist, Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

Principal Scientist, Division of Bacteriology and
Mycology

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