Effect of growth differentiation factor-9 and fibroblast growth factor-basic on small caprine oocyte development in vitro

P S P GUPTA; US PAVANNA SHREE; A DHALI; S NANDI

doi:10.56093/ijans.v88i12.85758

Authors

P S P GUPTA Principal Scientist, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru, Karnataka 560 030 India
US PAVANNA SHREE MVSc Scholar, Veterinary College, Hebbal, Bengaluru
A DHALI Principal Scientist, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru, Karnataka 560 030 India
S NANDI Principal Scientist, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru, Karnataka 560 030 India

https://doi.org/10.56093/ijans.v88i12.85758

Keywords:

bFGF, Caprine, GDF-9, In vitro development, Oocyte

Abstract

The study aimed to assess the effect of growth differentiation factor-9 (GDF-9) and fibroblast growth factorbasic (bFGF) on in vitro development of small (<126 μm diameter) caprine oocytes. Small oocytes were recovered from abattoir derived caprine ovaries and matured in vitro (24 h) in the presence of GDF-9 (0, 10, 20, 30 ng/ml) or bFGF (0, 10, 20, 30 ng/ml), and maturity and viability rates were assessed. A combination of both the growth factors (GDF-9, 30 ng/ml + bFGF, 20 ng/ml) was used to mature the oocytes in vitro (24 h). Subsequently oocytes were fertilized in vitro with cauda epidydimis sperm processed with BO medium (2×106/ml sperm). Cleavage and fertilization rates were assessed at 42 to 48 h post-insemination and morula/ blastocyst rate was assessed on 7 to 8 d post-fertilization. Maturation rate was significantly greater in oocytes cultured in media containing 30 ng/ml GDF-9 or 20 ng/ml bFGF compared to control. Maturation, fertilization, cleavage and morulae/blastocyst rates were significantly greater in oocytes cultured in combination of GDF-9 and bFGF compared to control. In conclusion, the supplementation of GDF-9 and bFGF in in vitro maturation (IVM) medium improved the maturation rate and embryo development of small caprine oocytes.

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References

Bayne R, Kinnell H L, Coutts S M, He J, Childs A J and Anderson R A. 2015. GDF9 is transiently expressed in oocytes before follicle formation in the human fetal ovary and is regulated by a novel NOBOX Transcript. PLoS ONE 10(3): e0119819. DOI: https://doi.org/10.1371/journal.pone.0119819

Binelli M and Murphy B D. 2010. Coordinated regulation of follicle development by germ and somatic cells. Reproduction Fertility and Development 22: 1–12. DOI: https://doi.org/10.1071/RD09218

Chang A S, Dale A N and Moley K H. 2005. Maternal diabetes adversely affects preovulatory oocyte maturation, development, and granulosa cell apoptosis. Endocrinology 146: 2445–53. DOI: https://doi.org/10.1210/en.2004-1472

Christine X Y, Gilchrist R B, Thompson J G and Lane M. 2008. Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice. Human Reproduction 23: 67–73. DOI: https://doi.org/10.1093/humrep/dem140

Convissar S, Armouti M, Fierro M, Winston N, Scoccia H, Zamah A M and Stocco C. 2017. Regulation of AMH by oocyte specific growth factors in human primary cumulus cells. Reproduction 154(6): 745–53. DOI: https://doi.org/10.1530/REP-17-0421

Fulka J Jr, Motlik J, Fulka J and Crozet N. 1985. Inhibition of nuclear maturation in fully grown porcine and mouse oocytes after their fusion with growing porcine oocytes. Journal of Experimental Zoology 235(2): 255–59. DOI: https://doi.org/10.1002/jez.1402350212

Gilchrist R B, Ritter L J, Myllymaa S, Kaivo-Oja N, Dragovic R A, Hickey T E et al. 2006. Molecular basis of oocyte-paracrine signalling that promotes granulosa cell proliferation. Journal of Cell Science 119: 3811–21. DOI: https://doi.org/10.1242/jcs.03105

Gupta P S P, Nandi S, Ravindranatha B M and Sarma P V. 2002a. Trypan blue staining to differentiate live and dead buffalo oocytes and its effect on embryo development in vitro. Buffalo Journal 18: 321–29.

Gupta P S P, Nandi S, Ravindranatha B M and Sarma P V. 2002b. In vitro maturation of buffalo oocytes with epidermal growth factor and fibroblast growth factor. Indian Journal of Animal Sciences 72: 23–26.

Mondal S, Mor A, Reddy I J, Nandi S and Gupta P S P. 2015. Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep. Brazilian Archives of Biology and Technology 58(4): 521–25. DOI: https://doi.org/10.1590/S1516-8913201500059

Ormond C M, Lima P F, Jardina-Sartor D T, Price C A and Buratini J. 2012. Effects of fibroblast growth factor 8 on cumulus expansion and nuclear maturation in bovine cumulus-oocyte complexes. Reproduction Fertility and Development 25: 276. DOI: https://doi.org/10.1071/RDv25n1Ab257

Su J, Wang Y, Zhang L, Wang B, Liu J, Luo Y, Guo Z, Quan F and Zhang Y. 2014. Oocyte-secreted factors in oocyte maturation media enhance subsequent development of bovine cloned embryos. Molecular Reproduction and Development 81: 341–49. DOI: https://doi.org/10.1002/mrd.22302

Valerio M P, Machado M, Jose Buratini, Jr., Zamberlam G, Amorim R L, Goncalves P and Price C A. 2010. Expression and function of fibroblast growth factor 18 in the ovarian follicle in cattle. Biology of Reproduction 83(3): 339–96. DOI: https://doi.org/10.1095/biolreprod.110.084277

Wang X, Schutzkus V, Huang W, Rosa G J and Khatib H. 2009. Analysis of segregation distortion and association of the bovine FGF2 with fertilization rate and early embryonic survival. Animal Genetics 40: 722–28. DOI: https://doi.org/10.1111/j.1365-2052.2009.01904.x

Yang M, Hall J, Fan Z, Regouski M, Meng Q, Rutigliano H M, Stott R, Rood K A, Panter K E and Polejaev I A. 2016. Oocytes from small and large follicles exhibit similar development competence following goat cloning despite their differences in meiotic and cytoplasmic maturation. Theriogenology 86(9): 2302–11. DOI: https://doi.org/10.1016/j.theriogenology.2016.07.026

Zhang K and Ealy A D. 2012. Supplementing fibroblast growth factor 2 during bovine oocyte in vitro maturation promotes subsequent embryonic development. Open Journal of Animal Science 2: 119–26. DOI: https://doi.org/10.4236/ojas.2012.22017

Zhang K, Hansen P J and Ealy A D. 2010. Fibroblast growth factor 10 enhances bovine oocyte maturation and developmental competence in vitro. Reproduction 140: 815–26. DOI: https://doi.org/10.1530/REP-10-0190