Haematological and biochemical alterations in native sheep experimentally infected with bluetongue virus serotype-2
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Authors
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J H KHORAJIYA
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K P SINGH
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PANKAJ BHATT
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M SAMINATHAN
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S TIWARI
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S A BHAT
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S VINEETHA
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M MAITY
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SHIBANI PANDA
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V K GUPTA
Keywords:
BTV-2 serotype, Experimental infection, Haematology, Native sheep, Serum biochemistryAbstract
The study was designed to determine the haematological and biochemical alterations in sero-negative native sheep following the experimental bluetongue virus serotype-2 (BTV-2) infection. The BTV infected group comprised 14 sheep inoculated with 6 ml of clarified virus containing 1×106/ml TCID50 of BTV-2 by intradermal route. The uninfected control group comprised 6 animals inoculated with 6 ml of cell culture medium without virus by intradermal route. The blood and serum samples were analyzed at 0, 1, 2, 3, 7, 11, 14, 21 and 45 days post-infection (dpi). Significant changes were observed in all the haematological and biochemical parameters studied. Marked leucopenia was observed from 2 to 7 dpi in BTV infected group. Significant leucocytosis was documented during 11 to 14 dpi in infected group. Significant thrombocytopenia was observed during 2 to 14 dpi whereas significantly low packed cell volume (PCV) and haemoglobin (Hb) values were observed between 3 and 21 dpi in BTV infected group. Differential leucocyte count revealed significantly low lymphocyte percentage on day 3 and high on day 11 in infected group. The various biochemical enzymes like alanine aminotransferase (ALT) showed significantlyhigh values during 3 to 21 dpi, aspartate aminotransferase (AST) during 3 to 21 dpi, alkaline phosphatise (ALP) during 3 to 11 dpi and creatine kinase (CK) during 7 to 14 dpi in BTV infected group. The result of our study demonstrated significantly decreased levels of total leucocyte count, total platelet count, haemoglobin and PCV values while significantly increased levels of ALT, AST, ALP and CK values in BTV infected group. On histopathological examination, spleen and lymph nodes showed depletion of lymphoid cells, liver and kidney showed degeneration, congestion and haemorrhage at many places. The BTV nucleic acid was detected from blood and tissues by RT-PCR. These findings indicated the damage to various soft tissue organs and muscles as a sequel to vascular endothelial damages caused by BTV.
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Ayanur A, Singh K P, Cherian S, Singh V, Nandi S, Saminathan M, Jaynudin K, SinghYadav J, Singh R and Tiwari A K. 2016. Sero-epidemiology and molecular detection of Bluetongue virus in Indian ruminants. Veterinaria Italiana 52(3–4): 305– 11.
Anjaneya A, Singh K P, Cherian S, Saminathan M, Singh R, Ramakrishnan M A, Maan S, Maan N S, Hemadri D, Rao P P, Putty K, Krishnajyothi Y and Mertens P P. 2018. Comparative neuropathology of major Indian bluetongue virus serotypes in a neonatal BALB/c mouse model. Journal of Comparative Pathology 162: 18–28. DOI: https://doi.org/10.1016/j.jcpa.2018.06.001
Boyd J W. 1983. The mechanisms relating to increases in plasma enzymes and isoenzymes in diseases of animals. Veterinary Clinical Pathology 12: 9–24. DOI: https://doi.org/10.1111/j.1939-165X.1983.tb00609.x
Chandra R, Kapoor D, Agarwal S R, Malhotra V, Sakhuja P and Sarin S K. 2002. Profile of asymptomatic chronic HBV infection in India. Indian Journal of Medical Research 116: 50–57
DeMaula C D, Leutenegger C M, Bonneau K R and MacLachlan N J. 2002. The role of endothelial cell-derived inflammatory and vasoactive mediators in the pathogenesis of bluetongue. Virology 296(2): 330–37. DOI: https://doi.org/10.1006/viro.2002.1476
Ellis J A, Luedke A J, Davis W C, Wechler S J, MacLachlan N J, Pratt D L and Elloit J D. 1990. T lymphocyte subset alterations following bluetongue virus infection in sheep and cattle. Veterinary Immunology and Immunopathology 24: 49–67. DOI: https://doi.org/10.1016/0165-2427(90)90077-6
Flanagan M, Johnson S J, Hoffmann D, Polkinghorne I G, Reid D J and Shepherd M A. 2008. Clinical pathology of Australian bluetongue virus serotype 16 infection in Merino sheep. Australian Veterinary Journal 70(3): 101–04. DOI: https://doi.org/10.1111/j.1751-0813.1993.tb03286.x
Gibbs E P J and Greiner E C. 1988. Bluetongue and epizootic haemorrhagic disease. Arboviruses: Epidemiology and Ecology 2: 39–70.
Hofmann M A, Renzullo S, Mader M, Chaignat V, Worwa G and Thuer B. 2008. Genetic characterization of Toggenburg orbivirus, a new bluetongue virus, from goats, Switzerland. Emerging Infectious Diseases 14: 1855–61. DOI: https://doi.org/10.3201/eid1412.080818
Jeggo M H, Corteyn A H, Taylor W P, Davidson W H and Gorman B M. 1986. Virulence of bluetongue virus for British sheep. Research in Veterinary Science 42: 24–28. DOI: https://doi.org/10.1016/S0034-5288(18)30651-9
Jenckel M, Bréard E, Schulz C, Sailleau C, Viarouge C, Hoffmann B, Höper D, Beer M and Zientara S. 2015. Complete coding genome sequence of putative novel bluetongue virus serotype DOI: https://doi.org/10.1128/genomeA.00016-15
Genome Announcement 3: e00016–15.
Kaneko J J, Harvey J and Wand Bruss L M. 1997. Clinical Biochemistry of Domestic Animals. 5th edn. Academic Press, London. pp. 303–25.
Luedke A J, Bowne J G, Jochim M M and Doyle C. 1964. Clinical and pathological features of bluetongue in sheep. American Journal of Veterinary Research 25: 963–70.
Maan S, Maan N S, Belaganahalli M N, Rao P P, Singh K P, Hemadri D, Putty K, Kumar A, Batra K, Krishnajyothi Y and Chandel B S. 2015. Full genome sequencing as a basis for molecular epidemiology studies of bluetongue virus in India. PLoS ONE 10: e0131257. DOI: https://doi.org/10.1371/journal.pone.0131257
Maan S, Maan N S, Nomikou K, Batten C, Antony F, Belaganahalli M N, Samy A M, Reda A A, Al-Rashid S A, El Batel M and Oura C A. 2011. Novel bluetongue virus serotype from Kuwait. Emerging Infectious Diseases 17: 886–89. DOI: https://doi.org/10.3201/eid1705.101742
MacLachlan N J. 2004. Bluetongue pathogenesis and duration of viraemia. Veterinaria Italiana 40(4): 462–67.
McColl K A and Gould A R. 1994. Bluetongue virus infection in sheep: haematological changes and detection by polymerase chain reaction. Australian Veterinary Journal 71(4): 97–101. DOI: https://doi.org/10.1111/j.1751-0813.1994.tb03346.x
Mellor P S. 1990. The replication of bluetongue virus in culicoides vectors. Current Topics in Microbiology and Immunology 162: 143–58. DOI: https://doi.org/10.1007/978-3-642-75247-6_6
Meyer G, Lacroux G, Lege R S, Top S and Goyeau K. 2009. Lethal bluetongue virus serotype-1 infection in llamas. Emerging Infectious Diseases 15: 608–10. DOI: https://doi.org/10.3201/eid1504.081514
Owens R J, Limn C and Roy P. 2004. Role of an arbovirus nonstructural protein in cellular pathogenesis and virus release. Journal of Virology 78: 6649–56. DOI: https://doi.org/10.1128/JVI.78.12.6649-6656.2004
Reed L J and Muench H. 1938. A simple method for estimating fifty percent endpoints. American Journal of Hygiene 21: 493– 97. DOI: https://doi.org/10.1093/oxfordjournals.aje.a118408
Saminathan M, Rana R, Ramakrishnan M A, Karthik K, Malik Y S and Dhama K. 2016. Prevalence, diagnosis, management and control of important diseases of ruminants with special reference to Indian scenario. Journal of Experimental Biology and Agricultural Sciences 4: 338–67. DOI: https://doi.org/10.18006/2016.4(3S).338.367
Sanchez-Cordon P J, Pleguezuelos F J, Perez de Diego A C, Gomez-Villamandos J C, Sanchez-Vizcaino J M, Ceron J J, Tecles F, Garfia B and Pedrera M. 2013. Comparative study of clinical courses, gross lesions, acute phase response and coagulation disorders in sheep inoculated with bluetongue virus serotype 1 and 8. Veterinary Microbiology 166: 184–94. DOI: https://doi.org/10.1016/j.vetmic.2013.05.032
Sapre S N. 1964. An outbreak of ‘bluetongue’ in goats and sheep in Maharashtra state. Veterinary Review 15: 69–71.
Singh K P, Umeshappa C S, Ahmed K A and Pandey A B. 2008. Haematological and biochemical response in native sheep experimentally infected with bluetongue virus serotype-23. Indian Journal of Animal Sciences 78: 8–12.
Valentine B A, Blue J T, Shelley S M and Cooper B J. 1990. Increased serum alanine aminotransferase activity associated with muscle necrosis in the dog. Journal Veterinary Internal Medicine 4: 140. http://www.oie.int/animal-health-in-the-world/oie-listed-diseases-2018/. OIE-listed diseases, infections and infestations in force in 2018. Accessed on 1st July 2018. DOI: https://doi.org/10.1111/j.1939-1676.1990.tb00886.x
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