Molecular characterization and sequence phylogenetic studies on Theileria annulata Mathura isolate based on TAMS and 18S gene

SANJEEV KUMAR; DAYA SHANKER; SANJHI PALIWAL; VIKRANT SUDAN; KAPIL KUMAR GUPTA; MUKESH SRIVASTAVA

doi:10.56093/ijans.v89i1.86382

Authors

SANJEEV KUMAR Senior Research Fellow, U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
DAYA SHANKER Professor, U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
SANJHI PALIWAL PhD Scholar, U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
VIKRANT SUDAN Assistant Professor, U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
KAPIL KUMAR GUPTA U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
MUKESH SRIVASTAVA U P Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India

https://doi.org/10.56093/ijans.v89i1.86382

Keywords:

Molecular characterization, TAMS gene, Theileria annulata, 18S gene

Abstract

The TAMS and 18S gene of local calf isolate from semiarid Mathura were amplified and used for phylogenetic analysis after custom sequencing. Results revealed phylogenetic association between Indian isolates in comparison to isolates across the world. Based on TAMS gene, the present isolates showed per cent homologies in the range 65.8â€“97.2% with various isolates across India and abroad. Likewise, 18S gene based per cent homologies of the present isolates varied from 70.5â€“100% with other isolates across India and abroad. The findings are important from molecular evolutionary point of view.

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