Genetic analysis of four indigenous duck populations of north-east India using microsatellite markers

BULA DAS; ARPANA DAS; ARUNDHATI PHOOKAN; G ZAMAN; A AZIZ; P J DAS; K BHARALI

doi:10.56093/ijans.v89i2.87343

Authors

BULA DAS Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
ARPANA DAS Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
ARUNDHATI PHOOKAN Assistant Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
G ZAMAN Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
A AZIZ Professor and Head, Assam Agricultural University, Guwahati, Asom 781 022 India
P J DAS Senior Scientist, ICAR-National Research Centre on Pig, Guwahati, Asom.
K BHARALI Former Research Fellow, Assam Agricultural University, Guwahati, Asom 781 022 India

https://doi.org/10.56093/ijans.v89i2.87343

Keywords:

Duck, Microsatellite, North-east India

Abstract

The genetic diversity and phylogenetic relationship of four duck populations, viz. Pati, Nageswari, Manipur and Tripura ducks of north eastern region of India were investigated by employing genetic polymorphisms of 25 microsatellites. The mean observed and effective number of alleles were found to be 2.670 and 1.949 respectively in all the four duck populations over 25 loci. The mean expected heterozygosity (He) was lower than the mean observed heterozygosity (Ho). Among all the populations, the He of Manipur ducks was the highest (0.443), followed by Tripura ducks (0.435), whereas Pati ducks was the lowest (0.396). The average He of all populations for all the loci was 0.420. The average PIC of all sites and populations was 0.366. However, CAUD007 in Manipur, Nageswari and Pati ducks, CAUD009 in Pati ducks, CAUD069 in Tripura ducks, and CAUD012 in all the four duck populations were found to be monomorphic. The test of Hardy- Weinberg Equilibrium showed that most of the loci in all the four populations were in Hardy-Weinberg disequilibrium. The FST value ranged between 0.000(CAUD012) and 0.512 (CAUD009) for each locus individually. The mean FST was 0.120. The genetic distance between Tripura ducks and Manipur ducks was the longest (0.2495) followed by Manipur and Nageswari ducks (0.2090) and, Nageswari and Pati ducks (0.1790).

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