Efficacy of soy-lecithin for replacing egg yolk in tris extender on quality of frozen buck semen

MANOJ KUMAR KALITA; SUDIP SINHA; BHARAT CHANDRA DEKA; RANJAN KUMAR BISWAS; LUITMANI BARKALITA; RUMI SAIKIA BORAH

doi:10.56093/ijans.v89i4.89137

Authors

MANOJ KUMAR KALITA PhD Scholar, Assam Agricultural University, Guwahati, Asom 781 022 India
SUDIP SINHA Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
BHARAT CHANDRA DEKA Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
RANJAN KUMAR BISWAS Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
LUITMANI BARKALITA Assistant Professor, Assam Agricultural University, Guwahati, Asom 781 022 India
RUMI SAIKIA BORAH Associate Professor, Assam Agricultural University, Guwahati, Asom 781 022 India

https://doi.org/10.56093/ijans.v89i4.89137

Keywords:

Egg yolk, Freezing, Goat semen, Post thaw quality, Soy-lecithin, Tris

Abstract

The present study undetaken to find the efficacy of incorporating soy-lecithin in tris extender for replacing egg yolk, a widely used animal component. Pooled ejaculates (40), 10 from each of four bucks maintained at Goat Research Station, Burnihat were used for freezing in tris extender containing 1% and 1.5% soy-lecithin and 20% egg yolk by adopting split sample technique following conventional method. The post thaw sperm motility in 1.5% soy-lecithin-tris extender (60.20Â±0.45%) was comparable with that of 20% egg yolk-tris (61.20Â±0.45%), the difference being non-significant. However, the post thaw values for live sperm, intact acrosome and hypo-osmotic swollen sperm were significantly lower in 1.5% soy-lecithin-tris as compared to that in 20% egg yolk-tris. The post thaw values were significantly higher for 1.5% than that for 1% soy-lecithin for all the parameters studied. It could be concluded that 1.5% soy-lecithin-tris extender has similar efficacy with that of 20% egg yolk-tris extender in respect of post thaw sperm motility; however, significantly lower post thaw values for remaining sperm qualities obtained with soy-lecithin in tris-based extender necessitate further trials comprising higher number of ejaculates from more bucks to find a suitable level of soy-lecithin for replacing egg yolk in tris extender for freezing of goat semen.

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