Differential expression of bovine major acute phase proteins, cytokines and metabolic indicator genes in clinical endometritis cows


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Authors

  • AYYASAMY MANIMARAN Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • ARUMUGAM KUMARESAN Principal Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • SOUVENDRA NATH SARKAR Principal Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • SANJANNA BOYA Research Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • L SREELA Research Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • P MOOVENTHAN Scientist, ICAR-National Institute of Biotic Stress Management, Baronda, Raipur, Chhattisgarh
  • PRATIK R WANKHADE Research Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v89i4.89139

Keywords:

Acute phase proteins, Clinical endometritis, Cows, Energy indicators, Gene expression, Pro-inflammatory cytokines

Abstract

Among the uterine diseases, clinical endometritis (CE) is a major challenge to livestock farming as it causes sub- or infertility problems in dairy animals. The aim of this study was to evaluate the expression of cytokines (IL- 1β, TNF-α, IL-6, and IL-8), acute phase proteins [APPs; haptoglobin (Hp), serum amyloid A (SAA) and alpha-1 acid glycoprotein (AGP)] and energy indicators [leptin and insulin-like growth factor (IGF)-1] genes in uterine tissue of CE affected cows. The uterine biopsy from CE cows (4) and non-endometritis cows (4) was processed for quantitative real-time PCR to study the mRNA expression of these innate immune molecules. We observed that mRNA expression of SAA, IL-1β, IL-8 and leptin genes were significantly up-regulated while, TNF-α and IGF-I genes were significantly down-regulated in CE cows. It can be concluded that bovine APPs, cytokines and energy indicators genes are differentially expressed in CE affected cows.

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References

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Submitted

2019-04-23

Published

2019-04-23

Issue

Vol. 89 No. 4 (2019)

Section

Articles

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The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

MANIMARAN, A., KUMARESAN, A., SARKAR, S. N., BOYA, S., SREELA, L., MOOVENTHAN, P., & WANKHADE, P. R. (2019). Differential expression of bovine major acute phase proteins, cytokines and metabolic indicator genes in clinical endometritis cows. The Indian Journal of Animal Sciences, 89(4), 402–406. https://doi.org/10.56093/ijans.v89i4.89139
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