Differential expression of bovine major acute phase proteins, cytokines and metabolic indicator genes in clinical endometritis cows


348 / 293

Authors

  • AYYASAMY MANIMARAN Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • ARUMUGAM KUMARESAN Principal Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • SOUVENDRA NATH SARKAR Principal Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • SANJANNA BOYA Research Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • L SREELA Research Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • P MOOVENTHAN Scientist, ICAR-National Institute of Biotic Stress Management, Baronda, Raipur, Chhattisgarh
  • PRATIK R WANKHADE Research Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v89i4.89139

Keywords:

Acute phase proteins, Clinical endometritis, Cows, Energy indicators, Gene expression, Pro-inflammatory cytokines

Abstract

Among the uterine diseases, clinical endometritis (CE) is a major challenge to livestock farming as it causes sub- or infertility problems in dairy animals. The aim of this study was to evaluate the expression of cytokines (IL- 1β, TNF-α, IL-6, and IL-8), acute phase proteins [APPs; haptoglobin (Hp), serum amyloid A (SAA) and alpha-1 acid glycoprotein (AGP)] and energy indicators [leptin and insulin-like growth factor (IGF)-1] genes in uterine tissue of CE affected cows. The uterine biopsy from CE cows (4) and non-endometritis cows (4) was processed for quantitative real-time PCR to study the mRNA expression of these innate immune molecules. We observed that mRNA expression of SAA, IL-1β, IL-8 and leptin genes were significantly up-regulated while, TNF-α and IGF-I genes were significantly down-regulated in CE cows. It can be concluded that bovine APPs, cytokines and energy indicators genes are differentially expressed in CE affected cows.

Downloads

Download data is not yet available.

References

1. Baumann H Z, Morella K K, Jahreis G P and Marinkovic S. 1990. Distinct regulation of the interleukin-1 and interleukin-6 response elements of the rat haptoglobin gene in rat and human hepatoma cells. Molecular and Cellular Biology 10(11): 5967– 76. DOI: https://doi.org/10.1128/MCB.10.11.5967

2. Beam S W and Butler W R. 1998. Energy balance, metabolic hormones, and early postpartum follicular development in dairy cows fed prilled lipid. Journal of Dairy Science 81(1): 121–31. DOI: https://doi.org/10.3168/jds.S0022-0302(98)75559-6

3. Chapwanya A, Meade K G, Doherty M L, Callanan J J and O’Farrelly C. 2013. Endometrial epithelial cells are potent producers of tracheal antimicrobial peptide and serum amyloid A3 gene expression in response to E. coli stimulation. Veterinary Immunology and Immunopathology 151(1–2): 157–62. DOI: https://doi.org/10.1016/j.vetimm.2012.09.042

4. Chapwanya A, Meade K G, Doherty M L, Callanan J J, Mee J F and O’Farrelly C. 2009. Histopathological and molecular evaluation of Holstein-Friesian cows postpartum: toward an improved understanding of uterine innate immunity. Theriogenology 71(9): 1396–407. DOI: https://doi.org/10.1016/j.theriogenology.2009.01.006

5. Davies D, Meade K G, Herath S, Eckersall P D, Gonzalez D, White J O, ConlanR S, O’Farrelly C and Sheldon I M. 2008. Toll-like receptor and antimicrobial peptide expression in the bovine endometrium. Reproductive Biology and Endocrinology 6(1): 53. DOI: https://doi.org/10.1186/1477-7827-6-53

6. Fischer C, Drillich M, Odau S, Heuwieser W, Einspanier R and Gabler C. 2010. Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis. Reproduction, Fertility and Development 22(5): 818–29. DOI: https://doi.org/10.1071/RD09120

7. Gabler C, Fischer C, Drillich M, Einspanier R and Heuwieser W. 2010. Time-dependent mRNA expression of selected pro- inflammatory factors in the endometrium of primiparous cows postpartum. Reproductive Biology and Endocrinology 8(1): 152. DOI: https://doi.org/10.1186/1477-7827-8-152

8. Galvao K N, Felippe M J B, Brittin S B, Sper R, Fraga M, Galvao J S, Caixeta L, Guard C L, Ricci A and Gilbert R O. 2012. Evaluation of cytokine expression by blood monocytes of lactating Holstein cows with or without postpartum uterine disease. Theriogenology 77(2): 356–72. DOI: https://doi.org/10.1016/j.theriogenology.2011.08.008

9. Galvao K N, Santos N R, Galvao J S and Gilbert R O. 2011. Association between endometritis and endometrial cytokine expression in postpartum Holstein cows. Theriogenology 76: 290–99. DOI: https://doi.org/10.1016/j.theriogenology.2011.02.006

10. Ghasemi F, Gonzalez-Cano P, Griebel P J and Palmer C. 2012. Proinflammatory cytokine gene expression in endometrial cytobrush samples harvested from cows with and without subclinical endometritis. Theriogenology 78(7): 1538–47. DOI: https://doi.org/10.1016/j.theriogenology.2012.06.022

11. Gilbert R O, Shin S T, Guard C L and Erb H N. 1998. Incidence of endometritis and effects on reproductive performance of dairy cows. Theriogenology 1(49): 251. DOI: https://doi.org/10.1016/S0093-691X(98)90604-5

12. Herath S, Fischer D P, Werling D, Williams E J, Lilly S T, Dobson H, Bryant C E and Sheldon I M. 2006. Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus. Endocrinology 147(1): 562–70. DOI: https://doi.org/10.1210/en.2005-1113

13. Ishikawa Y, Nakada K, Hagiwara K, Kirisawa R, Iwai H, Moriyoshi M and Sawamukai Y. 2004. Changes in interleukin- 6 concentration in peripheral blood of pre-and post-partum dairy cattle and its relationship to postpartum reproductive diseases. Journal of Veterinary Medical Science 66(11): 1403– 08. DOI: https://doi.org/10.1292/jvms.66.1403

14. Kasimanickam R K, Kasimanickam V R, Olsen J R, Jeffress E J, Moore D A and Kastelic J P. 2013. Associations among serum pro-and anti-inflammatory cytokines, metabolic mediators, body condition, and uterine disease in postpartum dairy cows. Reproductive Biology and Endocrinology 11(1): 103. DOI: https://doi.org/10.1186/1477-7827-11-103

15. Krakowski L and Zdzisinska B. 2007. Selected cytokines and acute phase proteins in heifers during the ovarian cycle course and in different pregnancy periods. Bulletin-Veterinary Institute in Pulawy 51(1): 31.

16. Lecchi C, Avallone G, Giurovich M, Roccabianca P and Ceciliani F. 2009. Extra hepatic expression of the acute phase protein alpha 1-acid glycoprotein in normal bovine tissues. Veterinary Journal 180(2): 256–58. DOI: https://doi.org/10.1016/j.tvjl.2007.12.027

17. Loyi T, Kumar H, Nandi S, Mathapati B S, Patra M K and Pattnaik B. 2013. Differential expression of pro-inflammatory cytokines in endometrial tissue of buffaloes with clinical and sub-clinical endometritis. Research in Veterinary Science 94(2): 336–40. DOI: https://doi.org/10.1016/j.rvsc.2012.09.008

18. Manimaran A, Kumaresan A, Jeyakumar S, Mohanty T K, Sejian V, Kumar N, Sreela L, Prakash M A, Mooventhan P, Anantharaj A and Das D N. 2016. Potential of acute phase proteins as predictor of postpartum uterine infections during transition period and its regulatory mechanism in dairy cattle. Veterinary World 9(1): 91–100. DOI: https://doi.org/10.14202/vetworld.2016.91-100

19. Muller-Doblies D, Arquint A, Schaller P, Heegaard P M, Hilbe M, Albini S, Abril C, Tobler K, Ehrensperger F, Peterhans E and Ackermann M. 2004. Innate immune responses of calves during transient infection with a noncytopathic strain of bovine viral diarrhea virus. Clinical and Diagnostic Laboratory Immunology 11(2): 302–12. DOI: https://doi.org/10.1128/CDLI.11.2.302-312.2004

20. Nino-Soto M I, Heriazon A, Quinton M, Miglior F, Thompson K and Mallard B A. 2008. Differential gene expression of high and low immune responder Canadian Holstein dairy cows. Animal Genomics for Animal Health 132: 315–20. DOI: https://doi.org/10.1159/000317277

21. Okuda K and Sakumoto R. 2003. Multiple roles of TNF super family m Biology and Endocrinology 1(1): 95. DOI: https://doi.org/10.1186/1477-7827-1-95

22. Pajovic S, Jones V E, Prowse K R, Berger F G and Baumann H. 1994. Species-specific changes in regulatory elements of mouse haptoglobin genes. Journal of Biological Chemistry 269(3): 2215–24. DOI: https://doi.org/10.1016/S0021-9258(17)42156-9

23. Rahman M M, Lecchi C, Avallone G, Roccabianca P, Sartorelli P and Ceciliani F. 2010. Lipopolysaccharide-binding protein: Local expression in bovine extrahepatic tissues. Veterinary Immunology and Immunopathology 137(1–2): 28–35. DOI: https://doi.org/10.1016/j.vetimm.2010.04.006

24. Roach D R, Bean A G, Demangel C, France M P, Briscoe H and Britton W J. 2002. TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection. Journal of Immunology 168(9): 4620–27. DOI: https://doi.org/10.4049/jimmunol.168.9.4620

25. Sheldon I M, Cronin J, Goetze L, Donofrio G and Schuberth H J. 2009. Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biology of Reproduction 81(6): 1025–32. DOI: https://doi.org/10.1095/biolreprod.109.077370

26. Sheldon I M, Lewis G S, LeBlanc S and Gilbert R O. 2006. Defining postpartum uterine disease in cattle. Theriogenology 65(8): 1516–30. DOI: https://doi.org/10.1016/j.theriogenology.2005.08.021

27. Swangchan-Uthai T, Lavender C R, Cheng Z, Fouladi-Nashta A A and Wathes D C. 2012. Time course of defense mechanisms in bovine endometrium in response to lipopolysaccharide. Biology of Reproduction 87(6): 135. DOI: https://doi.org/10.1095/biolreprod.112.102376

28. Wathes D C, Cheng Z, Chowdhury W, Fenwick M A, Fitzpatrick R, Morris D G, Patton J and Murphy J J. 2009. Negative energy balance alters global gene expression and immune responses in the uterus of postpartum dairy cows. Physiological Genomics 39(1): 1–13. DOI: https://doi.org/10.1152/physiolgenomics.00064.2009

29. Wathes D C, Cheng Z, Fenwick M A, Fitzpatrick R and Patton J. 2011. Influence of energy balance on the somatotrophic axis and matrix metalloproteinase expression in the endometrium of the postpartum dairy cow. Reproduction 141(2): 269–81. DOI: https://doi.org/10.1530/REP-10-0177

30. Williams E J, Fischer D P, Noakes D E, England G C, Rycroft A, Dobson H and Sheldon I M. 2007. The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow. Theriogenology 68(4): 549–59.embers in corpus luteum function. Reproductive DOI: https://doi.org/10.1016/j.theriogenology.2007.04.056

Downloads

Published

2019-04-23

Issue

Vol. 89 No. 4 (2019)

Section

Articles

License

Copyright (c) 2019 The Indian Journal of Animal Sciences

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

MANIMARAN, A., KUMARESAN, A., SARKAR, S. N., BOYA, S., SREELA, L., MOOVENTHAN, P., & WANKHADE, P. R. (2019). Differential expression of bovine major acute phase proteins, cytokines and metabolic indicator genes in clinical endometritis cows. The Indian Journal of Animal Sciences, 89(4), 402–406. https://doi.org/10.56093/ijans.v89i4.89139
Citation
Crossref
Scopus
Google Scholar
Europe PMC