Oxytetracycline is more suitable antibiotic for clinical endometritis cows

AYYASAMY MANIMARAN; H V RAGHU; A KUMARESAN; L SREELA; ASHA YADAV; S S LAYEK; P MOOVENTHAN; S CHAND; SOUVENDRA NATH SARKAR; M SIVARAM

doi:10.56093/ijans.v89i5.90013

Authors

AYYASAMY MANIMARAN Scientist, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India
H V RAGHU Scientist, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India
A KUMARESAN Principal Scientist, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India
L SREELA Research Scholar, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India
ASHA YADAV Research Scholar, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India
S S LAYEK Research Scholar, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India
P MOOVENTHAN Scientist, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India
S CHAND Technical officer, ICARNDRI, Karnal, Haryana
SOUVENDRA NATH SARKAR Principal Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh
M SIVARAM Principal Scientist, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560 030 India

https://doi.org/10.56093/ijans.v89i5.90013

Keywords:

Antibiotic sensitivity test, Clinical endometritis, E. coli, In vivo evaluation, Uterine pathogens

Abstract

We identified the bacterial isolates from the uterus of clinical endometritis affected cows and performed in vitro antibiotic sensitivity test (ABST) against major uterine pathogen (E. coli). We subsequently evaluated the sensitive antibiotics in clinical endometritis affected cows through fertility parameters, blood acute phase proteins (APPs; haptoglobin and serum amyloid A), cytokines level (IL-1Î² and TNF-Î±) and milk yield. We found 12.7% of E. coli among 63 bacterial isolates from the endometritis cows. ABST against E. coli revealed gentamicin as the most sensitive and oxytetracycline (OTC) as intermediately sensitive drug. Minimum inhibitory concentration (Î¼g/ml) of gentamicin and OTC against E. coli was 5.47 and 0.78, respectively. Gentamicin, OTC, and povidone-iodine (PI) were administered for 3 days through intrauterine route in endometritis cows (8 per group) for in vivo evaluation. The first service conception rate was higher in OTC treated cows with lesser days open, while interval between treatment and conception was nonsignificantly lower in PI-treated cows. The concentration of APPs and cytokines was significantly reduced in antibiotics treated cows but not in PI-treated groups. In conclusion, OTC was found to be more suitable than gentamicin for treatment of clinical endometritis and PI had differential effects on clinical endometritis cows.

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