Evaluation of anti-mullerian hormone in heifers and anestrus Murrah buffaloes

P M KEKAN; S D INGOLE; A S NAGVEKAR; S V BHARUCHA; S D KHARDE

doi:10.56093/ijans.v89i7.92012

Authors

P M KEKAN Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
S D INGOLE Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
A S NAGVEKAR Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
S V BHARUCHA Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
S D KHARDE Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India

https://doi.org/10.56093/ijans.v89i7.92012

Keywords:

Anestrus, Anti-Mullerian hormone, Buffaloes, Cattle, Heifer, Mullerian inhibiting substance

Abstract

An experiment was conducted to quantify the anti-mullerian hormone (AMH) concentration in heifers and anestrus Murrah buffaloes during fortnight blood collections for 3 months. Two groups of heifer and anestrus Murrah buffaloes were formed on the basis of record and actual per rectal examination. Serum AMH concentration was determined by ELISA method, using bovine AMH ELISA kit. Nonsignificant difference was recorded in AMH concentration (pg/ml) in heifer and anestrus buffaloes during fortnight collection (0, 15, 30, 45, 60 and 75). Highly significant individual variation was observed in heifer and anestrus buffaloes in this study. Heifers (6) with AMH concentration above 200 pg/ml were pregnant and 4 heifers with AMH concentration below 200 pg/ml were non pregnant. AMH concentration is a reliable phenotypic marker to predict the number of healthy follicles and oocytes, in ovaries and predict the future potential of heifers and young adult buffaloes. The cut-off value of AMH concentration in Murrah heifers buffaloes as a marker of fertility may be 200 pg/ml. However, study with more number of buffaloes is required to determine the accurate cut-off value of AMH concentration.

Downloads

Download data is not yet available.

References

Akbarinejad V, Gharagozlou F and Vojgani M. 2017. Temporal effect of maternal heat stress during gestation on the fertility and anti-mullerian hormone concentration of offspring in bovine. Theriogenology 99: 69–78. DOI: https://doi.org/10.1016/j.theriogenology.2017.05.018

Ali H E S, Kitahara G, Takahashi T, Mido S, Sadawy M, Kobayashi I, Hemmi K and Osawa T. 2017. Plasma anti-mullerian hormone profile in heifers from birth through puberty and relationship with puberty onset. Biology of Reproduction 97(1): 153–61. DOI: https://doi.org/10.1093/biolre/iox069

Baruselli P S, Batista E O S, Vieira L M and Souza A H. 2015. Relationship between follicle population, AMH concentration and fertility in cattle. Animal Reproduction 12(3): 487–97.

Batista E O S, Guerreiro B M, Freitas B G, Silva J C B, Vieira L M, Ferreira R M, Rezende R G, Basso A C, Lopes R N V R, Rennó F P, Souza A H and Baruselli P S. 2016. Plasma anti- mullerian hormone as a predictive endocrine marker to select Bos taurus (Holstein) and Bos indicus (Nelore) calves for in vitro embryo production. Domestic Animal Endocrinology 54: 1–9. DOI: https://doi.org/10.1016/j.domaniend.2015.08.001

Cate R L, Mattaliano R J, Hession C, Tizard R, Farber N M, Cheung A, Ninfa E G, Frey A Z, Gash D J, Chow E P, Fisher R A and Bertonis J M. 1986. Isolation of the bovine and human genes for mullerian inhibiting substance and expression of the human gene in animal cells. Cell 45: 685–98. DOI: https://doi.org/10.1016/0092-8674(86)90783-X

Center K, Dixon D, Looney C and Rorie R. 2018. Anti-Mullerian hormone and follicle counts as predictors of superovulatory response and embryo production in beef cattle. Advances in Reproductive Sciences 6: 22–33. DOI: https://doi.org/10.4236/arsci.2018.61003

Chachere J A. 2015. ‘Anti-Mullerian hormone concentrations as a predictor of superovulation response and conception rate in beef females’. Thesis submitted to Graduate and Professional Studies of Texas A & M University.

Das G K and Khan F A. 2010. Summer Anoestrus in Buffalo—A Review. Reproduction in Domestic Animals 45: 483–94. DOI: https://doi.org/10.1111/j.1439-0531.2010.01598.x

Fallat M E, Siow Y, Marra M, Cook C and Carrillo A. 1997. Mullerian-inhibiting substance in follicular fluid and serum: a comparison of patients with tubal factor infertility, polycystic ovary syndrome, and endometriosis. Fertility Sterility 67: 962– 65. DOI: https://doi.org/10.1016/S0015-0282(97)81417-3

Hirayama H, Naito A, Fukuda S, Fujii T, Asada M, Inaba Y, Takedomi T, Kawamata M, Moriyasu S and Kageyama S. 2017. Long-term changes in plasma anti-mullerian hormone concentration and the relationship with superovulatory response in Japanese Black cattle. Journal of Reproduction Development 63(1): 95–100. DOI: https://doi.org/10.1262/jrd.2016-019

Jimenez-Krassel F, Scheetz D, Neuder L, Ireland J, Pursley J, Smith G, Tempelman R, Ferris T, Roudebush W and Mossa F. 2015. Concentration of anti-mullerian hormone in dairy heifers is positively associated with productive herd life. Journal of Dairy Science 98: 3036–45. DOI: https://doi.org/10.3168/jds.2014-8130

Kavya K M, Sharma R K, Jerome A, Phulia S K and Singh I. 2017. Anti-Mullerian hormone and antral follicular count in early and delayed pubertal Murrah buffalo heifers. Livestock Science 198: 89–92. DOI: https://doi.org/10.1016/j.livsci.2017.02.013

Kumar P R, Singh S K, Kharche S D, Govindaraju C S, Behera B K, Shukla S N, Kumar H and Agarwal S K. 2014. Anestrus in cattle and buffalo: Indian perspective. Advances in Animal and Veterinary Science 2(3): 124–38. DOI: https://doi.org/10.14737/journal.aavs/2014/2.3.124.138

Noseir Wael M B. 2003. Ovarian follicular activity and hormonal profile during estrous cycle in cows: the development of 2 versus 3 waves. Reproductive Biology and Endocrinology 1: 1–6.

Rico C, Fabre S, Medigue C, Di Clemente N, Clement F, Bontoux M, Touze J L, Dupont M, Briant E, Remy B, Beckers J F and Monniaux D. 2009. Anti-Mullerian hormone is an endocrine marker of ovarian gonadotropin responsive follicles and can help to predict superovulatory responses in the cow. Biology of Reproduction 80: 50–59. DOI: https://doi.org/10.1095/biolreprod.108.072157

Rico C, Drouilhet L, Salvetti P, Dalbie‘s-Tran R, Jarrier P, Jean- Luc T, Pillet E, Ponsart C, Fabre S and Monniaux D. 2012. Determination of anti-mullerian hormone concentrations in blood as a tool to select Holstein donor cows for embryo production: from the laboratory to the farm. Reproduction Fertility and Development 24(7): 932–44. DOI: https://doi.org/10.1071/RD11290

Rorie R W, Newberry H R, Lester T D, Acharya M and Hansen C R. 2016. Anti-Mullerian hormone at weaning and breeding as a predictor of beef heifer fertility. Reproduction Fertility and Development 29(1): 111–12. DOI: https://doi.org/10.1071/RDv29n1Ab8

Silva T V, Santos J E P and Ribeiro E S. 2016. Plasma anti- mullerian hormone in dairy heifers and associations with reproductive performance in two reproductive programs for first artificial insemination. Journal of Animal Science 97(11): 6888–90.

Souza A H, Carvalho P D, Rozner A E, Vieira L M, Hackbart K S, Bender R W, Dresch A R, Verstegen J P, Shaver R D and Wiltbank M C. 2015. Relationship between circulating anti- mullerian hormone (AMH) and superovulatory response of high-producing dairy cows. Journal of Dairy Science 98: 169–78. DOI: https://doi.org/10.3168/jds.2014-8182

Visser J A, de Jong F H, Laven J S and Themmen A P. 2006. Anti- Mullerian hormone: A new marker for ovarian function. Reproduction 131: 1–9. DOI: https://doi.org/10.1530/rep.1.00529