Prevalence of bovine tropical theileriosis in sub-Himalyan region of northern India

ARUN RAJ M R; STUTI VATSYA; RAJEEV RANJAN KUMAR

doi:10.56093/ijans.v89i7.92015

Authors

ARUN RAJ M R MVSc Scholar, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
STUTI VATSYA Professor and Head, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
RAJEEV RANJAN KUMAR Assistant Professor, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India

https://doi.org/10.56093/ijans.v89i7.92015

Keywords:

AS-PCR assay, Bovine, India, LAMP assay, Theileria annulata, Therleriosis

Abstract

The prevalence of bovine tropical theileriosis (BTT) caused by Theileria annulata was studied in large ruminants of sub-Himalyan region of northern India using thin blood smear examination (TBE), polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP) assays. About 11.04% blood samples were found positive for BTT. Maximum seasonal prevalence of BTT was observed in summer followed by rainy season and winter. Month-wise maximum prevalence was recorded in May and minimum in January. Laboratory examination with Giemsa staining for Kochâ€™s blue bodies (KBB) and piroplasms revealed that 3.75% samples were positive for theileriosis. LAMP method gave the highest level of sensitivity followed by AS-PCR and TBE. Hence, it is suggested that LAMP can be a better molecular diagnostic tool for large scale epidemiological studies of bovine tropical theileriosis. Our results provide better understanding of this haemoprotozoan parasite and hence may help in devising timely control measures.

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