Exogenous kisspeptin (kp-10) resumes cyclicity in postpartum anestrus mithun cows

M H KHAN; P PERUMAL; S B HAZARIKA; E EZUNG

doi:10.56093/ijans.v89i8.93012

Authors

M H KHAN Principal Scientist, ICAR-National Research Centre on Mithun, Medziphema, Nagaland 797 106 India
P PERUMAL Scientist, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India
S B HAZARIKA Junior Research Fellow, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India
E EZUNG Junior Research Fellow, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India

https://doi.org/10.56093/ijans.v89i8.93012

Keywords:

Anestrus, Endocrinological profiles, Estrus induction, Follicular development, Kiss 1, Mithun, Post-partum

Abstract

Present study was conducted to assess the effect of exogenous kisspeptin administration on estrus induction, Kiss1gene expression, endocrinological profiles and follicular development in post partum (PP) anestrus mithun cows. Animals who failed to resume cyclicity after day 90â€“100 of parturition were selected. Experimental cows were examined thoroughly through per-rectal as well as ultrasonograpic examination to confirm the anestrous status. PP anestrous healthy mithun cows (12), aged 5â€“6 years (body condition score: 5â€“6 of 10) were selected with uniform anestrus status and randomly divided into 2 groups namely treatment (Gr I) and control (Gr II), each group consist 6 cows. Anestrus cows in Gr 1 were injected with kisspeptin @ 1.30 Î¼g per kg body weight at 3 day interval for 21 days after 90â€“100 days of post parturition whereas in control group, normal saline was injected as placebo. Blood samples were collected on the days of injection and at estrus in both the groups. Trans-rectal ultrasonography was done at 3 days interval till onset of estrus and ovulation to study the follicular development. Exogenous kisspeptin administration caused significantly early resumption of cyclicity in treatment as compared to control (24.64Â±10.43 vs 66.56Â±14.66 days) and significantly increased kiss1 and GPR54 mRNA expression in treatment as compared to control on the day of estrus (1.943Â±0.29 vs 0.424Â±0.062 and 1.84Â±0.31 vs 0.416Â±0.082, respectively). Similarly, circulating level of estradiol and follicle stimulating hormone (FSH) increased gradually after exogenous administration of kisspeptin, which reached peak on the day of estrus in treatment group (25.36Â±1.27 pg/ml and 15.65Â±1.22 ng/ml, respectively) whereas no significant difference was observed between days of treatment in control group except on the day of estrus (11.29Â±1.76 pg/ml and 9.86Â±1.06 ng/ml, respectively). Level of estradiol and FSH on the day of estrus was significantly higher in treatment as compared to control whereas non-significant difference was observed in plasma progesterone concentration. Number of medium and large follicles increased in treatment whereas only small follicles were observed in control group. Improved endocrinological profiles, follicle development and kiss gene profiles in post partum anestrous mithun cows following exogenous kisspeptin indicates that kisspeptin inducted or resumed the cyclicity in early.

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References

Caraty A, Smith J T, Lomet D, Ben S S, Morrissey A, Cognie J, Doughton B, Baril G, Briant C and Clarke I J. 2007. Kisspeptin synchronizes pre-ovulatory surges in cyclical ewes and causes ovulation in seasonally acyclic ewes. Endocrinology 148: 5258–67. DOI: https://doi.org/10.1210/en.2007-0554

Chaudhari C F, Suthar B N, Sharma V K, Dabas V S, Chaudhari N F and Panchasara H H. 2012. Estrus induction and fertility response in delayed pubertal Kankrej heifers treated with norgestomet ear implant. Veterinary World 5: 453–58. DOI: https://doi.org/10.5455/vetworld.2012.453-458

Dhali A, Mishra D P, Mech A, Karunakaran M and Rajkhowa C. 2005. Endocrine control of estrous cycle in mithun (Bos frontalis). Theriogenology 64: 2010–21. DOI: https://doi.org/10.1016/j.theriogenology.2005.05.002

Ezzat A A, Saito H, Sawada T, Yaegashi T, Yamashita T, Hirata T, Sawai K and Hashizume T. 2009. Characteristics of the stimulatory effect of kisspeptin-10 on the secretion of luteinizing hormone, follicle-stimulating hormone and growth hormone in pre pubertal male and female cattle. Journal of Reproduction and Development 55: 650–54. DOI: https://doi.org/10.1262/jrd.20255

Giasuddin M, Huque K S and Alam J. 2003. Reproductive potential of Gayal (Bos frontalis) under semi-intensive management. Asian Australasian Journal of Animal Science 3: 331–34. DOI: https://doi.org/10.5713/ajas.2003.331

Greives T J, Mason A O, Scotti M A, Levine J, Ketterson E D, Kriegsfeld L J and Demas G E. 2007. Environmental control of kisspeptin: implications for seasonal reproduction. Endocrinology 148: 1158–66. DOI: https://doi.org/10.1210/en.2006-1249

Joseph A D, Chad D F, Brian K W and James L S. 2015. Reproduction and beyond, kisspeptin in ruminants. Journal of Animal Science and Biotechnology 23: 6. DOI: https://doi.org/10.1186/s40104-015-0021-4

Keen K L, Wegner F H, Bloom S R, Ghatei M A and Terasawa E. 2008. An increase in kisspeptin-54 release occurs with the pubertal increase in luteinizing hormone-releasing hormone- 1 release in the stalk-median eminence of female rhesus monkeys in vivo. Endocrinology 149: 4151–57. DOI: https://doi.org/10.1210/en.2008-0231

Knobil E. 1980. The neuroendocrine control of the menstrual cycle. Recent Progress in Hormone Research 36: 53–88. DOI: https://doi.org/10.1016/B978-0-12-571136-4.50008-5

Li Q, Rao A, Clarke I J and Smith J T. 2012. Seasonal variation in the gonadotropin-releasing hormone response to kisspeptin in sheep: possible kisspeptin regulation of the kisspeptin receptor. Neuroendocrinology 96: 212–21. DOI: https://doi.org/10.1159/000335998

Livak K J and Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (- delta delta c (t)) method. Elsevier Science (USA) 25: 402–08. DOI: https://doi.org/10.1006/meth.2001.1262

Moenter S M, Brand R M, Midgley A R and Karsch F J. 1992. Dynamics of gonadotropin-releasing hormone release during a pulse. Endocrinology 130: 503–10 DOI: https://doi.org/10.1210/endo.130.1.1727719

Naniwa Y, Nakatsukasa K, Setsuda S, Oishi S, Fujii N, Matsuda F, Uenoyama Y, Tsukamura H, Maeda K and Ohkura S. 2013. Effects of full-length kisspeptin administration on follicular development in Japanese black beef cows. Journal of Reproduction and Development 59: 588–94. DOI: https://doi.org/10.1262/jrd.2013-064

Navarro V M, Castellano J M, Fernandez-Fernandez R, Barreiro M L, Rao J, Sanchez-Criado J E, Aguilar E, Dieguez C, Pinilla L and Tena-Sempere M. 2004. Developmental and hormonally regulated messenger ribonucleic acid expression of Kiss-1 and its putative receptor, GPR54 in rat hypothalamus and potent luteinizing hormone-releasing activity of Kiss-1 peptide. Endocrinology 145: 4565–74. DOI: https://doi.org/10.1210/en.2004-0413

Plant T M, Ramaswamy S and Dipietro M J. 2006. Repetitive activation of hypothalamic G protein-coupled receptor 54 with intravenous pulses of kisspeptin in the juvenile monkey (Macaca mulatta) elicits a sustained train of gonadotropin- releasing hormone discharges. Endocrinology 147: 1007–13. DOI: https://doi.org/10.1210/en.2005-1261

Pratheesh M D, Radhika S, Harish C, Anoopraj R and Justin D. 2013. Role of Kisspeptin in puberty and reproduction. Vet Scan 6: 87.

Revel F G, Saboureau M, Masson-Pevet M, Pevet P, Mikkelsen J D and Simonneaux V. 2006. Kiss-1: a likely candidate for the photoperiodic control of reproduction in seasonal breeders. Chronobiology International 23: 277–87. DOI: https://doi.org/10.1080/07420520500521939

Shisode M G, Khanvilkar A V, Kulkarni M D, Samant S R, Yadav G B and Bawaskar M S. 2009. Mithun: The Pride animal of North-eastern hilly region of India. Veterinary World 12: 480– 81.

Smith J T, Dungan H M, Stoll E A, Gottsch M L, Braun R E, Eacker S M, Clifton D K and Steiner R A. 2005. Differential regulation of Kiss-1 mRNA expression by sex steroids in the brain of the male mouse. Endocrinology 146: 2976–84. DOI: https://doi.org/10.1210/en.2005-0323

Smith J T, Pereira A, Rao A and Clarke I J. 2006. Kisspeptin-10 stimulates luteinizing hormone release from the ovine pituitary gland in vitro. Frontier in Neuroendocrinology 27: 77–78. DOI: https://doi.org/10.1016/j.yfrne.2006.03.162

Snedecor S W and Cochran W G. 1994. Statistical Methods. 8th edn. University Press, Ames, IA, USA.

Tena-Sempere M. 2006. GPR54 and kisspeptin in reproduction. Human Reproduction Update 12: 631–39. DOI: https://doi.org/10.1093/humupd/dml023

Tovar S, Vazquez M J, Navarro V M, Fernandez-Fernandez R, Castellano J M, Vigo E, Rao J, Casanueva F F, Aguilar E, Pinilla L, Dieguez C and Tena-Sempere M. 2006. Effects of single or repeated intravenous administration of kisspeptin upon dynamic LH secretion in conscious male rats. Endocrinology 147: 2696–2704. DOI: https://doi.org/10.1210/en.2005-1397

Yavas Y and Walton J S. 2000. Postpartum acyclicity in suckled beef cows. Theriogenology 54: 25–55. DOI: https://doi.org/10.1016/S0093-691X(00)00323-X