Puerarin protects rat liver and kidney against cadmium-induced oxidative stress

JICANG WANG; HUALI ZHU; CAI ZHANG; HONGWEI WANG; ZIJUN YANG; ZONGPING LIU

doi:10.56093/ijans.v89i9.93750

Authors

JICANG WANG Henan University of Science and Technology, Kaiyuan Avenue, 471 023, Luoyang, PR China
HUALI ZHU Henan University of Science and Technology, Kaiyuan Avenue, 471 023, Luoyang, PR China
CAI ZHANG Henan University of Science and Technology, Kaiyuan Avenue, 471 023, Luoyang, PR China
HONGWEI WANG Henan University of Science and Technology, Kaiyuan Avenue, 471 023, Luoyang, PR China
ZIJUN YANG Henan University of Science and Technology, Kaiyuan Avenue, 471 023, Luoyang, PR China
ZONGPING LIU College of Veterinary Medicine, Yangzhou University, China.

https://doi.org/10.56093/ijans.v89i9.93750

Keywords:

Cadmium, Kidney, Liver, Oxidative stress, Puerarin

Abstract

Oxidative stress is thought to be involved in cadmium (Cd) induced toxicity. This study examined the possible protective effect of puerarin on cadmium chloride (CdCl2, 2 mg/kg b.w.) induced toxicity in male rats. Male SD rats were treated with either intraperitoneal Cd and/or oral puerarin (100 mg/kg. b.w.) for 4 weeks. The results demonstrated that exposure to Cd led to an increase in the level of BUN, ALT and AST in serum. Cadmium raised the concentrations of MDA and GSH, and decreased antioxidants activities (SOD, CAT, and GSH-Px) in the liver and kidney. Conversely, administration of puerarin markedly attenuated Cd-induced biochemical alterations in serum, liver, and kidney tissues. These results suggest that puerarin exerts protective effects against Cd toxicity attributable to its antioxidant actions.

Downloads

Download data is not yet available.

References

Calderon J, Ortiz-Perez D, Yanez L and Diaz-Barriga F. 2003. Human exposure to metals. Pathways of exposure, biomarkers of effect, and host factors. Ecotoxicology and Environmental Safety 56(1): 93–103. DOI: https://doi.org/10.1016/S0147-6513(03)00053-8

Cao Z, Fang Y, Lu Y, Tan D, Du C, Li Y, Ma Q, Yu J, Chen M and Zhou C. 2017. Melatonin alleviates cadmium-induced liver injury by inhibiting the TXNIP-NLRP3 inflammasome. Journal of Pineal Research 62(3): e12389. DOI: https://doi.org/10.1111/jpi.12389

Djuric A, Begic A, Gobeljic B, Ninkovic M, Stanojevic I, Vojvodic D, Pantelic A, Zebic G, Prokic V and Dejanovic B. 2015. Oxidative stress, bioelements and androgen status in testes of rats sub-acutely exposed to cadmium. Food and Chemical Toxicology 86: 25–33. DOI: https://doi.org/10.1016/j.fct.2015.09.004

El-Boshy M, Ashshi A, Gaith M, Qusty N, Bokhary T, Al Taweel N and Abdelhady M. 2017. Studies on the protective effect of the artichoke (Cynara scolymus) leaf extract against cadmium toxicity-induced oxidative stress, hepato-renal damage, and immunosuppressive and hematological disorders in rats. Environmental Science and Pollution Research 24(13): 12372– 83. DOI: https://doi.org/10.1007/s11356-017-8876-x

Gaurav D, Preet S and Dua K. 2011. Protective influence of dietary nutrients on antioxidant defense system in the blood of rats treated with cadmium. Advances in Applied Science Research 2(2): 69–78.

Hirano S and Suzuki K T. 1996. Exposure, metabolism, and toxicity of rare earths and related compounds. Environmental Health Perspectives 104(Suppl 1): 85. DOI: https://doi.org/10.1289/ehp.96104s185

Horiguchi H, Sato M, Konno N and Fukushima M. 1996. Long- term cadmium exposure induces anemia in rats through hypoinduction of erythropoietin in the kidneys. Archives of Toxicology 71(1–2): 11–19. DOI: https://doi.org/10.1007/s002040050352

Hwang Y P, Choi C Y, Chung Y C, Jeon S S and Jeong H G. 2007. Protective effects of puerarin on carbon tetrachloride-induced hepatotoxicity. Archives of Pharmacal Research 30(10): 1309– 17. DOI: https://doi.org/10.1007/BF02980272

Kidambi S S, Lee D-K and Ramamoorthy A. 2003. Interaction of Cd and Zn with biologically important ligands characterized using solid-state NMR and ab initio calculations. Inorganic Chemistry 42(9): 3142–51. DOI: https://doi.org/10.1021/ic026287d

Li R, Liang T, He Q, Guo C, Xu L, Zhang K and Duan X. 2013. Puerarin, isolated from Kudzu root (Willd.), attenuates hepatocellular cytotoxicity and regulates the GSK-3b/NF-êB pathway for exerting the hepato-protection against chronic alcohol-induced liver injury in rats. International Immunopharmacology 17(1): 71–78. DOI: https://doi.org/10.1016/j.intimp.2013.05.023

Liu C M, Ma J Q and Sun Y Z. 2011. Protective role of puerarin on lead-induced alterations of the hepatic glutathione antioxidant system and hyperlipidemia in rats. Food and Chemical Toxicology 49(12): 3119–27. DOI: https://doi.org/10.1016/j.fct.2011.09.007

Liu C M, Ma J Q and Sun Y Z. 2012. Puerarin protects rat kidney from lead-induced apoptosis by modulating the PI3K/Akt/ eNOS pathway. Toxicology and Applied Pharmacology 258(3): 330–42. DOI: https://doi.org/10.1016/j.taap.2011.11.015

Massanyi P, Lukáè N, Uhrin V, Toman R, Pivko J, Rafay J, Forgács Z and Somosy Z. 2007. Female reproductive toxicology of cadmium. Acta Biologica Hungarica 58(3): 287–99. DOI: https://doi.org/10.1556/ABiol.58.2007.3.5

Nna V U, Usman U Z, Ofutet E O and Owu D U. 2017. Quercetin exerts preventive, ameliorative and prophylactic effects on cadmium chloride-induced oxidative stress in the uterus and ovaries of female Wistar rats. Food and Chemical Toxicology 102:143–55. DOI: https://doi.org/10.1016/j.fct.2017.02.010

Nwokocha C R, Owu D U, Nwokocha M I, Ufearo C S and Iwuala M O. 2012. Comparative study on the efficacy of Allium sativum (garlic) in reducing some heavy metal accumulation in liver of wistar rats. Food and Chemical Toxicology 50(2): 222–26. DOI: https://doi.org/10.1016/j.fct.2011.11.003

Patra R, Swarup D and Senapati S. 1999. Effects of cadmium on lipid peroxides and superoxide dismutase in hepatic, renal and testicular tissue of rats. Veterinary and Human Toxicology 41(2): 65–67.

Prasain J K, Peng N, Rajbhandari R and Wyss J M. 2012. The chinese Pueraria root extract (Pueraria lobata) ameliorates impaired glucose and lipid metabolism in obese mice. Phytomedicine 20(1): 17–23. DOI: https://doi.org/10.1016/j.phymed.2012.09.017

Sarkar A, Ravindran G and Krishnamurthy V. 2013. A brief review on the effect of cadmium toxicity: from cellular to organ level. International Journal of Bio-Technology and Research 3(1): 17–36.

Satarug S and Moore M R. 2004. Adverse health effects of chronic exposure to low-level cadmium in foodstuffs and cigarette smoke. Environmental Health Perspectives 112(10): 1099–103. DOI: https://doi.org/10.1289/ehp.6751

Stohs S J, Bagchi D, Hassoun E and Bagchi M. 2001. Oxidative mechanisms in the toxicity of chromium and cadmium ions. Journal of Environmental Pathology, Toxicology and Oncology 20(2): 77–88. DOI: https://doi.org/10.1615/JEnvironPatholToxicolOncol.v20.i2.10

Valko M, Morris H and Cronin M. 2005. Metals, toxicity and oxidative stress. Current Medicinal Chemistry 12(10): 1161– 208. DOI: https://doi.org/10.2174/0929867053764635

Wang J, Zhu H, Cai Z, Wang H and Yang Z. 2018. Baicalein ameliorates cadmium-induced hepatic and renal oxidative damage in rats. Indian Journal of Animal Research 53(4): 523– 27. DOI: https://doi.org/10.18805/ijar.B-853

Wu D and Cederbaum A I. 2003. Alcohol, oxidative stress, and free radical damage. Alcohol Research and Health 27: 277– 84.

Xie W and Du L. 2011. Diabetes is an inflammatory disease: Evidence from traditional Chinese medicines. Diabetes, Obesity and Metabolism 13(4): 289–301. DOI: https://doi.org/10.1111/j.1463-1326.2010.01336.x

Xu B, Xu Z, Deng Y and Yang J. 2010. Protective effects of Chlorpromazine and Verapamil against cadmium-induced kidney damage in vivo. Experimental and Toxicologic Pathology 62(1): 27–34. DOI: https://doi.org/10.1016/j.etp.2008.12.009