Effect of resveratrol supplementation to Triladyl® on the quality of ram chilled semen

MOHSEN AL-MUTARY

doi:10.56093/ijans.v89i9.93752

Authors

MOHSEN AL-MUTARY Imam Abdulrahman Bin Faisal University, P.O. Box 2375, Dammam 31451, Saudi Arabia

https://doi.org/10.56093/ijans.v89i9.93752

Keywords:

Najdi ram, Resistance, Resveratrol, Spermatozoa

Abstract

The present investigation aimed to estimate the in vitro effects of resveratrol (RES) on the quality of chilled ram semen. Resveratrol was added to Triladyl® in three different concentration as 0 (control), 200 or 400 μM. Semen was collected from three Najdi rams, pooled and extended with one of three previous extenders for a final concentration of 600×106 sperm/ml. Then, the diluted semen was cooled gradually, and stored at 4°C. The total motility, viability, plasma membrane integrity and acrosome integrity were assessed at 0, 24, 72 and 120 h of storage. The thermal incubation test was evaluated at day 7. The survival time, total motility and the progressive motility were registered at day 15 of storage. After 4 h of incubation, the percentage of live sperm was significantly lower in the control group than in other groups. Results of survival time, total motility and progressive motility at day 15 of liquid storage were significantly higher in RES groups than that of the control. Based on the results of incubation resistance, survival time, total and progressive motility, it could be concluded that adding 200 and 400 μM of RES to Triladyl extender can improve the quality of ram semen during chilled storage at 4°C.

Downloads

Download data is not yet available.

References

Abd El-Hack M E, Alagawany M, Ragab M F, Tiwari R, Karthik K, Dhama K and Adel M. 2016. Beneficial impacts of thymol essential oil on health and production of animals, fish and poultry: a review. Journal of Essential Oil Research 28(5): 365–82. DOI: https://doi.org/10.1080/10412905.2016.1153002

Ahmad M, Nasrullah R and Ahmad N. 2015. Effect of cooling rate and equilibration time on pre-freeze and post-thaw survival of buck sperm. Cryobiology 70: 233–38. DOI: https://doi.org/10.1016/j.cryobiol.2015.03.002

Alagawany M M, Farag M R, Dhama K, Abd El-Hack M E, Tiwari R and Alam G M. 2015. Mechanisms and beneficial applications of resveratrol as feed additive in animal and poultry nutrition: A review. International Journal of Pharmacology 11(3): 213–21. DOI: https://doi.org/10.3923/ijp.2015.213.221

Amidi F, Pazhohan A, Shabani Nashtaei M, Khodarahmian M and Nekoonam S. 2016. The role of antioxidants in sperm freezing: a review. Cell Tissue Bank 17: 745–56. DOI: https://doi.org/10.1007/s10561-016-9566-5

Ball B A, Medina V, Gravance C G and Baumbe J. 2001. Effect of antioxidants on preservation of motility,viability and acrosomal integrity of equine spermatozoa during storage at 5 degrees C. Theriogenology 56: 577–89. DOI: https://doi.org/10.1016/S0093-691X(01)00590-8

Baur J A and Sinclair D A. 2006. Therapeutic potential of resveratrol: the in vivo evidence. Nature Reviews Drug Discovery 5: 493–506. DOI: https://doi.org/10.1038/nrd2060

Benhenia K, Rahab H, Smadi M A, Benmakhlouf H, Lamara A, Idres T and Iguer-Ouada M. 2018. Beneficial and harmful effects of cyclodextrin-vitamin E complex on cryopreserved ram sperm. Animal Reproduction Science (in Press). DOI: https://doi.org/10.1016/j.anireprosci.2018.06.004

Branco C S, Garcez M E, Pasqualotto F F, Erdtman B and Salvador M. 2010. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology 60: 235–37. DOI: https://doi.org/10.1016/j.cryobiol.2009.10.012

Collodel G, Federico M G, Geminiani M, Martini S, Bonechi C, Rossi C, Figura N and Moretti E. 2011. Effect of trans- resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reproduction Toxicology 31: 239–46. DOI: https://doi.org/10.1016/j.reprotox.2010.11.010

Fremont L. 2000. Biological effects of resveratrol. Life Sciences 66: 663–73. DOI: https://doi.org/10.1016/S0024-3205(99)00410-5

Giaretta E, Bucci D, Mari G, Galeati G, Love C C, Tamanini C and Spinaci M. 2014. Is resveratrol effective in protecting stallion cooled semen? Journal of Equine Veterinary Science 34: 1307–12. DOI: https://doi.org/10.1016/j.jevs.2014.09.011

Gil J, Fierro S, Bentancur O and Olivera-Muzante J. 2011. Chilled storage of ram semen improves with the addition of egg yolk and glycerol to milk-basedextenders. Reproduction of Domestic Animals 46: 503–07. DOI: https://doi.org/10.1111/j.1439-0531.2010.01697.x

Kankofer M, Kolm G, Aurich J and Aurich C. 2005. Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5°C. Theriogenology 63: 1354–65. DOI: https://doi.org/10.1016/j.theriogenology.2004.07.005

Kasimanickam R, Kasimanickam V, Pelzer K D and Dascanio J J. 2007. Effect of breed and sperm concentration on the changes in structural, functional and motility parameters of ram-lamb spermatozoa during storage at 4°C. Animal Reproduction Sciece 101: 60–73. DOI: https://doi.org/10.1016/j.anireprosci.2006.09.001

Kasimanickam R, Kasimanickam V, Tibary A and Pelzer K. 2011. Effect of semen extenders on sperm parameters of ram semen during liquid storage at 4°C. Small Ruminant Research 99: 208–13. DOI: https://doi.org/10.1016/j.smallrumres.2011.03.057

King M E, Mckelvey W A, Dingwall W S, Matthews K P, Gebbie F E, Mylne M J, Stewart E and Robinson J J. 2004. Lambing rates and litter sizes following intrauterine or cervical insemination of frozen/thawed semen with or without oxytocin administration. Theriogenology 62: 1236–44. DOI: https://doi.org/10.1016/j.theriogenology.2004.01.009

Kovács A and Foote R H. 1992. Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biotechnic and Histochemistry 67: 119–24. DOI: https://doi.org/10.3109/10520299209110020

Li H, Zhang X G, Fang Q, Liu Q, Du R R, Yang G S, Wang L Q and Hu J H. 2017. Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C. Animal Science Journal 88: 1692–99. DOI: https://doi.org/10.1111/asj.12865

Longobardi V, Zullo G, Salzano A, De Canditiis C, Cammarano A, De Luise L, Puzio M V, Neglia G and Gasparrini B. 2017. Resveratrol prevents capacitation-like changes and improves in vitro fertilizing capability of buffalo frozen-thawed sperm. Theriogenology 88: 1–8. DOI: https://doi.org/10.1016/j.theriogenology.2016.09.046

Mata-Campuzano M, Alvarez-Rodriguez M, Tamayo-Canul J, Lopez-Uruena E, De Paz P, Anel L, Martinez-Pastor F and Alvarez M. 2014. Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time. Animal Reproduction Science 151: 137–47. DOI: https://doi.org/10.1016/j.anireprosci.2014.10.006

Matheny K, Hopper R, King H, Memili E and Nicodemus M. 2015. Effects of resveratrol on post-thaw quality of stallion sperm. Journal of Equine Veterinary Science 35: 443. DOI: https://doi.org/10.1016/j.jevs.2015.03.152

Naijian H R, Kohram H, Shahneh A Z, Sharafi M and Bucak M N. 2013. Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze-thaw process. Cryobiology 66: 151–55. DOI: https://doi.org/10.1016/j.cryobiol.2012.12.010

Ortega Ferrusola C, Gonzalez Fernandez L, Morrell J M, Salazar Sandoval C, Macias Garcia B, Rodriguez-Martinez H, Tapia J A and Pena F J. 2009. Lipid peroxidation, assessed with BODIPY-C11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic- like changes. Reproduction 138: 55–63. DOI: https://doi.org/10.1530/REP-08-0484

Pagl R, Aurich C and Kankofer M. 2006. Anti-oxidative status and semen quality during cooled storage in stallions. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine 53: 486–89. DOI: https://doi.org/10.1111/j.1439-0442.2006.00879.x

Peris S I, Bilodeau J F, Dufour M and Bailey J L. 2007. Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm. Molecular Reproduction and Development 74: 878–92. DOI: https://doi.org/10.1002/mrd.20686

Ramu S and Jeyendran R S. 2013. The hypo-osmotic swelling test for evaluation of sperm membrane integrity. Methods in Molecular Biology 927: 21–25. DOI: https://doi.org/10.1007/978-1-62703-038-0_3

Sarlos P, Molnar A, Kokai M, Gabor G and Ratky J. 2002. Comparative evaluation of the effect of antioxidants in the conservation of ram semen. Acta Veterinaria Hungarica 50: 235–45. DOI: https://doi.org/10.1556/avet.50.2002.2.13

Schuh H. 1992. Comparison between liquid and deep-frozen semen for artificial insemination in developing and developed countries. World Animal Review 70–71: 44–53.

Shipley C F B, Buckrell B C, Mylne M J A, Pollard J and Hunton J R. 2007. Chapter 86 - Artificial insemination and embryo transfer in sheep, pp. 629–41. Current Therapy in large animal theriogenology (Second edition). (Eds.) Youngquist R S and Threlfall W R. Saint Louis: W.B. Saunders. DOI: https://doi.org/10.1016/B978-072169323-1.50089-1

Tasdemir U, Buyukleblebici S, Tuncer P B, Coskun E, Ozgurtas T, Aydin F N, Buyukleblebici O and Gurcan I S. 2013. Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters. Cryobiology 66: 38–42. DOI: https://doi.org/10.1016/j.cryobiol.2012.10.006

Toker M B, Alcay S, Gokce E and Ustuner B. 2016. Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology 72: 205–09. DOI: https://doi.org/10.1016/j.cryobiol.2016.05.001

Tvrda E, Kovacik A, Tusimova E, Massanyi P and Lukac N. 2015. Resveratrol offers protection to oxidative stress induced by ferrous ascorbate in bovine spermatozoa. Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances and Environmental Engineering 50: 1440–51. DOI: https://doi.org/10.1080/10934529.2015.1071153

Vichas L, Tsakmakidis I A, Vafiadis D, Tsousis G, Malama E and Boscos C M. 2017. The effect of antioxidant agents’ addition and freezing method on quality parameters of frozen thawed ram semen. Cell and Tissue Banking 19: 113–21. DOI: https://doi.org/10.1007/s10561-017-9633-6

Watson P F. 1975. Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Veterinary Record 97: 12–15. DOI: https://doi.org/10.1136/vr.97.1.12