Seasonal variation in expression pattern of heat shock factor genes in Ovis aries and Capra hircus

LATIKA JAISWAL; SACHINANDAN DE; RAVI KANT SINGH

doi:10.56093/ijans.v89i9.93771

Authors

LATIKA JAISWAL Senior Research Fellow, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
SACHINANDAN DE Principal Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
RAVI KANT SINGH Professor and Director, Amity Institute of Biotechnology, Amity University, Raipur, Chhattisgarh.

https://doi.org/10.56093/ijans.v89i9.93771

Keywords:

Goat, Heat shock factors, Seasonal changes, Sheep, Stress

Abstract

In many dairy animals the correlation between longevity and stress resistance has been observed, which suggests that, for the regulation of lifespan, the ability to sense and respond to environmental challenges is important. Therefore it is necessary to observe the role of heat shock factors (HSFs), in the regulation of longevity which acts as a master transcriptional regulator of stress-inducible gene expression and protein folding homeostasis. Exposure to heat stress causes changes which have a substantial impact on production and productivity. Therefore the four major mammalian HSF genes, HSF-1, 2, 4, and 5 have been studied in sheep and goat. Major objective of this study was to analyze the expression status of these genes in sheep and goat using gene-specific primers. Changes in the gene expression profile of these two species were noted by quantitative real-time PCR (qRT-PCR). The expression level in both the species has been studied and it was found that the level of HSF-1, 2, 4 and 5 mRNA was higher in testis compared to all the tissues examined. Moreover, they are expressed in a wide range of tissues but their expression was variable. The analysis of seasonal changes in blood profile in goat and sheep showed an up-regulation in HSF-4 and HSF-5 genes in winter. The study implicates that the intricate balance of different HSFs is adjusted to minimize the effect of seasonal changes in environmental conditions. These findings enlighten our understanding of the complex, context-dependent regulation of HSF gene expression under normal and stressful conditions.

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References

Ahn S G and Thiele D J. 2003. Redox regulation of mammalian heat shock factor-1 is essential for Hsp gene activation and protection from stress. Genes and development 17: 56–28. DOI: https://doi.org/10.1101/gad.1044503

Akerfelt M, Morimoto R I and Sistonen L. 2010. Heat shock factors: integrators of cell stress, development and lifespan. Nature reviews Molecular cell biology, 11: 545–55. DOI: https://doi.org/10.1038/nrm2938

Akerfelt M, Trouillet D, Mezger V and Sistonen L. 2007. Heat shock factors at a crossroad between stress and development. Annals of the New York Academy of Sciences 1113: 15–27. DOI: https://doi.org/10.1196/annals.1391.005

Attia N S. 2016. Physiological, hematological and biochemical alterations in heat stressed goats. Benha Veterinary Medical Journal 31: 56–62. DOI: https://doi.org/10.21608/bvmj.2016.31261

Fiorenza M T. 1995. Complex expression of murine heat shock transcription factors. Nucleic acids research 23: 467–74. DOI: https://doi.org/10.1093/nar/23.3.467

Guisbert G. 2013. Identification of a tissue-selective heat shock response regulatory network. PLoS Genetics 9(4): e1003466. DOI: https://doi.org/10.1371/journal.pgen.1003466

Lal S, Brahma B, Gohain M, Mohanta D, De B, Chopra M, Dass G, Vats A, Upadhyay R C, Datta T K and De S. 2015. Splice variants and seasonal expression of buffalo HSF genes. Cell stress and chaperones 20: 545–54. DOI: https://doi.org/10.1007/s12192-014-0563-y

Livak K J and Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(- Delta Delta C(T)) method. Methods 25: 402–08. DOI: https://doi.org/10.1006/meth.2001.1262

Morimoto R I, Tissieres A and Georgopoulos C. 1994. The biology of the heat shock proteins and molecular chaperones. Cold spring harbor laboratory press, Cold Spring Harbor, NY. 26: 610.

Naidu S D and Dinkova Kostova A T. 2017. Regulation of the mammalian heat shock factor-1. The FEBS Journal 284: 1606– 27. DOI: https://doi.org/10.1111/febs.13999

Nardone A, Ronchi B, Lacetera N, Ranieri M and Bernabucci U. 2010. Effects of climate changes on animal production and sustainability of livestock systems. Livestock Science 130: 57– 69. DOI: https://doi.org/10.1016/j.livsci.2010.02.011

Prasad K V, Taiyab A, Jyothi D, Srinivas U K and Sreedhar A S. 2007. Heat shock transcription factors regulate heat induced cell death in a rat histiocytoma. Journal of Biosciences 2: 585– 93. DOI: https://doi.org/10.1007/s12038-007-0058-4

Saju J M, Hossain M S, Liew W C, Pradhan A, Thevasagayam N M, Tan L S, Anand A, Olsson P E and Orbán L. 2018. Heat shock factor-5 is essential for spermatogenesis in Zebrafish. Cell Reports 25: 3252–61. DOI: https://doi.org/10.1016/j.celrep.2018.11.090

Sarangi S. 2018. Adaptability of goats to heat stress: A review. The Pharma Innovation Journal 7: 1114–26

Sejian V. 2013. Climate change: Impact on production and reproduction, adaptation mechanisms and mitigation strategies in small ruminants: A review. The Indian Journal of Small Ruminants 19: 1–21.

Sorger P K. 1991. Heat shock factor and heat shock response. Cell 65: 363–66. DOI: https://doi.org/10.1016/0092-8674(91)90452-5

Thiruvenkadan A K, Ramanujam R and Dharan M. 2013. Buffalo genetic resources of India and their conservation. Buffalo Bulletin 32: 227–35.

Valasek M A and Repa J J. 2005. The power of real-time PCR. Advances in physiology education 29: 151–59. DOI: https://doi.org/10.1152/advan.00019.2005

Wang G, Ying Z, Jin X, Tu N, Zhang Y, Phillips M, Moskophidis D and Mivechi N F. 2004. Essential requirement for both HSF- 1 and HSF-2 transcriptional activity in spermatogenesis and male fertility. Genesis 38: 66–80. DOI: https://doi.org/10.1002/gene.20005

West J W. 1999. Nutritional strategies for managing the heat- stressed dairy cow. Journal of Animal Science 77: 21–35. DOI: https://doi.org/10.2527/1997.77suppl_221x