Immunogenicity of recombinant outer membrane protein (OmpW) of Pasteurella multocida serogroup B:2 in mouse model
Abstract views: 124 / PDF downloads: 73
https://doi.org/10.56093/ijans.v89i10.94999
Keywords:
Haemorrhagic septicaemia, Immunogenicity, Mouse model, Pasteurella multocida, Recombinant OmpW proteinAbstract
Haemorrhagic septicaemia (HS) caused by Pasteurella multocida serogroup B:2, in cattle and buffalo especially in tropical regions of Asian and African countries, is known to possess several outer membrane proteins (OMPs) as virulent factors which are being targeted to evaluate their immunogenicity and protective efficacy as candidate antigens for vaccine. In the present study, ompW gene encoding for OmpW protein of P. multocida serogroup B:2 strain P52, an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant OmpW fusion protein (~37 kDa) including histidine tag was purified by affinity chromatography under denaturing condition and confirmed by Western blotting. Further, mice immunized with rOmpW (50μg/ dose) along with FCA/FIA resulted in antigen specific IgG antibodies as well as subtypes (IgG1 and IgG2a). The study indicated the potential possibilities to use the rOmpW antigen in developing subunit vaccine for HS as well as other diseases caused by members of Pasteurellaceae.Downloads
References
Ahmad T A, Rammah S S, Sheweita S A, Haroun M and El-Sayed L H. 2014. Development of immunization trials against Pasteurella multocida. Vaccine 32: 909–17. DOI: https://doi.org/10.1016/j.vaccine.2013.11.068
Albrecht R, Zeth K, Soding J, Lupas A and Linke D. 2006. Expression, crystallization and preliminary X-ray crystallographic studies of the outer membrane protein OmpW from Escherichia coli. Acta Crystallographica 62: 415–8. DOI: https://doi.org/10.1107/S1744309106010190
Bisgaard M. 1993. Ecology and significance of Pasteurellaceae in animals. International Journal of Medical Microbiology 279: 7–26. DOI: https://doi.org/10.1016/S0934-8840(11)80487-1
Biswas A, Shivachandra S B, Saxena M K, Kumar A A, Singh V P and Srivastava S K. 2004. Molecular variability among strains of Pasteurella multocida isolated from an outbreak of haemorrhagic septicaemia in India. Veterinary Research Communication 28: 287–98. DOI: https://doi.org/10.1023/B:VERC.0000026656.77847.5b
Boyce J D, Cullen P A, Nguyen V, Wilkie I and Adler B. 2006. Analysis of the Pasteurella multocida outer membrane sub- proteome and its response to the in vivo environment of the natural host. Proteomics 6: 870–80. DOI: https://doi.org/10.1002/pmic.200401342
Chacko N, Mohanty N N, Biswas S K, Chand K, Yogisharadhya R, Pandey A B, Mondal B and Shivachandra S B. 2015. A coiled-coil motif in non-structural protein 3 (NS3) of bluetongue virus forms an oligomer. Virus Genes 51: 244– 51. DOI: https://doi.org/10.1007/s11262-015-1230-9
Das M, Chopra A K, Cantu J M and Peterson J W. 1998. Antisera to selected outer membrane proteins of Vibrio cholerae protect against challenge with homologous and heterologous strains of V. cholerae. FEMS Immunology and Medical Microbiology 22: 303–8. DOI: https://doi.org/10.1111/j.1574-695X.1998.tb01219.x
Fernández Rojas M A, Vaca S, Reyes López M, de la Garza M, Aguilar Romero F, Zenteno E, Soriano Vargas E and Negrete Abascal E. 2014. Outer membrane vesicles of Pasteurella multocida contain virulence factors. Microbiology Open 3: 711–7. DOI: https://doi.org/10.1002/mbo3.201
Hatfaludi T, Al-Hasani K, Boyce J D and Adler B. 2010. Outer membrane proteins of Pasteurella multocida. Veterinary Microbiology 144: 1–17. DOI: https://doi.org/10.1016/j.vetmic.2010.01.027
Hatfaludi T, Al-Hasani K, Gong L, Boyce J D, Ford M, Wilkie I W, Quinsey N, Dunstone M A, Hoke D E and Adler B. 2012. Screening of 71 Pasteurella multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE. PLoS One 7: p.e39973. DOI: https://doi.org/10.1371/journal.pone.0039973
Hong H, Patel D R, Tamm L K and van den Berg B. 2006. The outer membrane protein OmpW forms an eight-stranded - barrel with a hydrophobic channel. Journal of Biological Chemistry 281: 7568–77. DOI: https://doi.org/10.1074/jbc.M512365200
Kumar A, Yogisharadhya R, Ramakrishnan M A, Viswas K N and Shivachandra S B. 2013. Structural analysis and cross- protective efficacy of recombinant 87 kDa outer membrane protein (Omp87) of Pasteurella multocida serogroup B:2. Microbial Pathogenesis 65: 48–56. DOI: https://doi.org/10.1016/j.micpath.2013.09.007
Maiti B, Shetty M, Shekar M, Karunasagar I and Karunasagar I. 2012. Evaluation of two outer membrane proteins, Aha1 and OmpW of Aeromonas hydrophila as vaccine candidate for common carp. Veterinary Immunology Immunopathology 149: 298–301. DOI: https://doi.org/10.1016/j.vetimm.2012.07.013
May B J, Zhang Q, Li L L, Paustian M L, Whittam T S and Kapur V. 2001. Complete genomic sequence of Pasteurella multocida, Pm70. Proceedings of National Academy of Sciences USA 98: 3460–5. DOI: https://doi.org/10.1073/pnas.051634598
Moustafa A M, Seemann T, Gladman S, Adler B, Harper M, Boyce J D and Bennett M D. 2015. Comparative genomic analysis of Asian haemorrhagic septicaemia—associated strains of Pasteurella multocida identifies more than 90 haemorrhagic septicaemia-specific genes. PLoS One 10: e0130296. DOI: https://doi.org/10.1371/journal.pone.0130296
Nandi B, Nandy R K, Mukhopadhyay S, Nair G B, Shimada T and Ghose A C. 2000. Rapid method for species—specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW. Journal of Clinical Microbiology 38: 4145–51. DOI: https://doi.org/10.1128/JCM.38.11.4145-4151.2000
Nandi B, Nandy R K, Sarkar A and Ghose A C. 2005. Structural features, properties and regulation of the outer-membrane protein W (OmpW) of Vibrio cholerae. Microbiology 151: 2975–86. DOI: https://doi.org/10.1099/mic.0.27995-0
Paustian M L, May B J and Kapur V. 2001. Pasteurella multocida gene expression in response to iron limitation. Infection and Immunity 69: 4109–15. DOI: https://doi.org/10.1128/IAI.69.6.4109-4115.2001
Shivachandra S B, Kumar A, Mohanty N N and Yogisharadhya R. 2017. Immunogenicity of recombinant Omp16 protein of Pasteurella multocida B:2 in mouse model. Indian Journal of Animal Science 87: 29–34.
Shivachandra S B, Kumar A, Yogisharadhya R and Viswas K N. 2014. Immunogenicity of highly conserved recombinant VacJ outer membrane lipoprotein of Pasteurella multocida. Vaccine 32: 290–6. DOI: https://doi.org/10.1016/j.vaccine.2013.10.075
Shivachandra S B, Viswas K N and Kumar A A. 2011. A review on haemorrhagic septicaemia in cattle and buffalo. Animal Health Research Reviews 12: 67–82. DOI: https://doi.org/10.1017/S146625231100003X
Shivachandra S B, Yogisharadhya R, Kumar A, Mohanty N N and Nagaleekar V K. 2015.
Recombinant transferrin binding protein A (TbpA) fragments of Pasteurella multocida serogroup B:2 provide variable protection following homologous challenge in mouse model. Research in Veterinary Science 98: 1–6. DOI: https://doi.org/10.1016/j.rvsc.2014.11.013
Shivachandra S B, Yogisharadya R, Ahuja A and Bhanuprakash V. 2012. Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli. Research in Veterinary Science 93: 1128–31. DOI: https://doi.org/10.1016/j.rvsc.2012.02.010
Stanczak P, Zhang Q, Horst R, Serrano P and Wüthrich K. 2012. Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles. Journal of Biomolecules NMR 54: 129– 33. DOI: https://doi.org/10.1007/s10858-012-9658-x
Verma R and Jaiswal T N. 1998. Haemorrhagic septicaemia vaccines. Vaccine 16: 1184–90. DOI: https://doi.org/10.1016/S0264-410X(98)80118-7
Wilkie I W, Harper M, Boyce J D and Adler B. 2012. Pasteurella multocida: diseases and pathogenesis. Current Topics in Microbiology and Immunology 361: 1–22. DOI: https://doi.org/10.1007/82_2012_216
Wu X B, Tian L H, Zou H J, Wang C Y, Yu Z Q, Tang C H, Zhao F K and Pan J Y. 2013. Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis. Research in Microbiology 164: 848–55. DOI: https://doi.org/10.1016/j.resmic.2013.06.008
Yogisharadhya R, Mohanty N N, Chacko N, Mondal M, Chanda M M and Shivachandra S B. 2018. Sequence and structural analysis of OmpW protein of Pasteurella multocida strains reveal evolutionary conservation among members of Pasteurellaceae along with its homologues. Gene Reports 14: 36–44. DOI: https://doi.org/10.1016/j.genrep.2018.11.004
Zhao X, Liu Q, Xiao K, Hu Y, Liu X, Li Y and Kong Q. 2016. Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity. BMC Microbiology 16: 125. DOI: https://doi.org/10.1186/s12866-016-0739-y
Downloads
Submitted
Published
Issue
Section
License
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.