Growth hormone 1 and insulin 2 gene polymorphism in Egyptian chickens

HEBA AMABD EL-KADER; HEBA ISHAFEY; EMEL-KOMY EMEL-KOMY; SALLY SALAM; KARIMA FMAHROUS

doi:10.56093/ijans.v89i10.95013

Authors

HEBA AMABD EL-KADER Associate Professor, National Research Centre, El Buhouth St., 12311 Dokki, Cairo, Egypt
HEBA ISHAFEY Professor, National Research Centre, El Buhouth St., 12311 Dokki, Cairo, Egypt
EMEL-KOMY EMEL-KOMY Researcher, National Research Centre, El Buhouth St., 12311 Dokki, Cairo, Egypt
SALLY SALAM Professor, National Research Centre, El Buhouth St., 12311 Dokki, Cairo, Egypt
KARIMA FMAHROUS Professor, National Research Centre, El Buhouth St., 12311 Dokki, Cairo, Egypt

https://doi.org/10.56093/ijans.v89i10.95013

Keywords:

Chicken, Egypt, GH1, Insulin 2, SNPs

Abstract

The genetic polymorphism of two genes related to growth production in four chicken breeds (Dokki 4, Inshas, El-Salam and Mandarah) were studied. PCR-RFLP and single nucleotide polymorphism (SNPs) analysis were studied in the two genes namely GH1 and insulin 2. Blood samples were collected randomly from 84 chickens, DNA was extracted and genotypes for two genes were detected using PCR-RFLP analysis. The locus GH1 (467 bp) showed two genotypes (GG, AG) in four chicken breeds due to the presence of two Msp1 restriction sites (C^CGG) in intron1. The sequence analysis revealed two substitutions (C/T) in Dokki 4 and Inshas breeds, G/A substitution in Dokki 4 and Mandarah breeds and insertion of T nucleotide in four chicken breeds. In insulin 2 gene (371 bp), two genotypes (TT, TC) were recorded in four chicken breeds due to presence of one restriction site in intron1. The nucleotide substitutions (A/G) and (T/C) were observed in all Egyptian chicken breeds except Inshas breed and A/ T transversion was observed in all breeds. The sequences of detected SNPs were submitted to GenBank database with the accession numbers MG906782â€“MG906789 in GH1 gene and the accession numbers MG906790â€“MG906791 in insulin 2 gene. In conclusion, the presence of certain polymorphisms could increase the phenotypic variety and will be supportive in selection and breeding programs.

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References

Abdlhag M A, Zhang T, Fan Q C, Zhang X Q, Zhang G X, Wang J Y, Wei Y and Wang Y J. 2015. Single nucleotide polymorphisms associated with growth traits in Jinghai yellow chickens. Genetics and Molecular Research 14(4): 16169– 77. DOI: https://doi.org/10.4238/2015.December.8.6

Anh N T, Kunhareang S and Duangjinda M. 2015. Association of chicken growth hormones and insulin-like growth factor gene polymorphisms with growth performance and carcass traits in thai broilers. Asian Australasian Journal of Animal Sciences 28(12): 1686–95. DOI: https://doi.org/10.5713/ajas.15.0028

Bassam G M A and Dihya H H A. 2016. Effect of G1705ASNP in growth hormone gene on the productive and physiological performance in broiler chicken. Iraqi Journal of Biotechnology 15(1): 33–45.

Clark A G. 2004. The role of haplotypes in candidate gene studies. Genetic Epidemiology 27:. DOI: https://doi.org/10.1002/gepi.20025

Darabi A, Fayazi J, Roshanfekr H and Nasiri M. 2010. Investigation of growth hormone gene polymorphism using PCR-RFLP technique in native poultry in Khuzestan province. Journal of Animal and Veterinary Advances 9(2): 255–57. DOI: https://doi.org/10.3923/javaa.2010.255.257

Heba I S, Aboelhassan M D, EL-Komy E M, Ragaa E A and Karima F M. 2017. SNP of cGH gene in egyptian chicken breeds at Msp I site. Biosciences Biotechnology Research Asia 14(1): 33–41. DOI: https://doi.org/10.13005/bbra/2414

Ipe S C, Zhang X and Leung F C. 2001. Genomic growth hormone gene polymorphism in native Chinese chicken. Experimental Biology and Medicine 226: 458–62. DOI: https://doi.org/10.1177/153537020122600511

Kadlec J, Hosnedlová B, RehouV, Èíte J, Veèere L and Hanusová L. 2011. Insulin-like growth factor-1 gene polymorphism and its association with growth and slaughter characteristics in broiler chickens. Journal of Agrobiology 28(2): 157–63. DOI: https://doi.org/10.2478/v10146-011-0017-4

Khoa D V A, Khang N T K, Ngu N T, Matey J, Loan H T P and Thuy N T D. 2013. Single nucleotide polymorphisms in Gh, Ghr, Ghsr and insulin candidate genes in chicken breeds of vietnam. Greener Journal of Agricultural Science 3(10): 716– 24. DOI: https://doi.org/10.15580/GJAS.2013.3.042613589

Kuhnlein U, Ni L and Zadworny D. 1997. DNA polymorphisms in the chicken growth hormone gene: Response to selection for disease resistance and association with egg production. Journal of Animal Genetics 28: 116–23. DOI: https://doi.org/10.1111/j.1365-2052.1997.00076.x

Lei M, Luo C, Peng X, Fang M, Nie Q, Zhang D, Yang G and Zhang X. 2007. Polymorphism of growth-correlated genes associated with fatness and muscle fiber traits in chickens. Journal of Poultry Science 86: 835–42. DOI: https://doi.org/10.1093/ps/86.5.835

Mehdi A and Reza F A. 2012. Single nucleotide polymorphisms in intron 1 of growth hormone gene and it’s association with economic important traits in Iranian fars native fowl. Annals of Biological Research 3: 4028–32.

Miller S A, Dykes D D and Polesky H F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16: 12–15. DOI: https://doi.org/10.1093/nar/16.3.1215

Morris R W and Kaplan N L. 2002. On the advantage of haplotype analysis in the presence of multiple disease susceptibility alleles. Genetic Epidemiology 23: 221–33. DOI: https://doi.org/10.1002/gepi.10200

Nie Q, Lei M, Ouyang J, Zeng H, Yang G and Zhang X. 2005. Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography. Genetics Selection Evolution 37: 339–60. DOI: https://doi.org/10.1186/1297-9686-37-4-339

Qui F F, Nie Q H, Luo C L, Zhang D X, Lin S M and Zhang X Q. 2006. Association of single nucleotide polymorphisms of the insulin gene with chicken early growth and fat deposition. Poultry Science 85: 980–85. DOI: https://doi.org/10.1093/ps/85.6.980

Shahnaz S, Shadma F, Rank D N, Khanna K and Joshi C G. 2008. Growth hormone gene polymorphism and its correlation with different traits in Bantam and White Leghorn chicken. Indian Journal of Poultry Science 43(2): 123–27.

Tanmankaurd G V P, Ravikumar P S, Bajwa I S and Trehan P K. 2008. PCR-RFLP of growth hormone gene in meat type chicken. Indian Journal of Poultry Science 43(2): 129–31.

Wilcox G. 2005. Insulin and insulin resistance. Clinical Biochemistry Reviews 26: 19–39.

Zhao W, Qiao N, Wang X, Chen Q, Cheng J, Xu Q and Chen G. 2011. Comparative genomic analysis of growth hormone gene in geese. Animal Science Journal 82: 62–66. DOI: https://doi.org/10.1111/j.1740-0929.2010.00812.x