Evaluation of anti-Mullerian hormone, antral follicle count and progesterone concentration during estrous cycle in Murrah buffaloes

P M KEKAN; S D INGOLE; A S NAGVEKAR; S V BHARUCHA; S D KHARDE; D P KADAM; C N GALDHAR; P S MASARE; S G DESHMUKH

doi:10.56093/ijans.v89i11.95860

Authors

P M KEKAN Assistant Professor, Department of Veterinary Physiology, College of Veterinary and Animal Sciences, Parbhani
S D INGOLE Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
A S NAGVEKAR Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
S V BHARUCHA Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
S D KHARDE Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
D P KADAM Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
C N GALDHAR Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
P S MASARE Assistant Professor, Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
S G DESHMUKH Assistant Professor, Department of Gynecology and Obstetrics, PGIAS, Akola.

https://doi.org/10.56093/ijans.v89i11.95860

Keywords:

Anti-Mullerian hormone, Antral follicle count, Buffaloes, Estrous cycle, Progesterone

Abstract

An experiment was conducted to study the anti-Mullerian hormone (AMH) concentration, antral follicle count (AFC) and progesterone (P4) concentration during estrous cycle in Murrah buffaloes. Seven animals of 5â€“10 years were selected for the study. All the animals were synchronized as per the ovsync protocol and the pair of ovary of each animal was scanned by ultrasonography on 0, 3rd, 7th, 11th, 14th, 17th and 21st day of estrous cycle. Blood was collected on the same day to evaluate serum AMH and P4 concentration. The nonsignificant difference was observed in antral follicle count of 3â€“5 mm, 5â€“8 mm and total follicles count (> 3 mm), whereas, significantly lower mean values of >8 mm of antral follicles were recorded on the day of estrus (0.14 Â±0.14) as compared to 3rd, 7th, 11th, 14th, 17th and 21st day of estrous cycle. The significant individual variation was recorded in average mean of 3 - 5 mm and total antral follicle count (>3 mm). However, the difference for 5 - 8 mm and >8 mm was nonsignificant. The positive correlation of low and high antral follicle count was observed in the present study. Two animals of low antral follicle count remained non pregnant and two animals showed 3 follicular waves during estrous cycle. The nonsignificant difference was recorded in AMH concentration during estrous cycle, whereas, individual variation in AMH concentration differ significantly. The progesterone concentration showed significant increase and decrease in values according to the stages of estrous cycle.

Downloads

Download data is not yet available.

References

Akbarinejad V, Gharagozlou F and Vojgani M. 2017. Temporal effect of maternal heat stress during gestation on the fertility and anti-mullerian hormone concentration of offspring in bovine Theriogenology 99: 69–78. DOI: https://doi.org/10.1016/j.theriogenology.2017.05.018

Burns D S, Jimenez-Krassel F, Ireland J L H, Knight P G and Ireland J J. 2005. Numbers of antral follicles during follicular waves in cattle: evidence for high variation among animals, very high repeatability in individuals, and an inverse association with serum follicle-stimulating hormone concentrations Biology of Reproduction 73 54–62 DOI: https://doi.org/10.1095/biolreprod.104.036277

Cate R L, Mattaliano, R J, Hession C, Tizard R, Farber N M, Cheung A, Ninfa E G, Frey A Z, Gash D J, Chow E P, Fisher R A and Bertonis J M. 1986. Isolation of the bovine and human genes for mullerian inhibiting substance and expression of the human gene in animal cells. Cell 45: 685–98 DOI: https://doi.org/10.1016/0092-8674(86)90783-X

Center K, Dixon D, Looney C and Rorie R. 2018. Anti-Mullerian hormone and follicle counts as predictors of superovulatory response and embryo production in beef cattle. Advances in Reproductive Sciences 6 22–33 DOI: https://doi.org/10.4236/arsci.2018.61003

Durlinger AL, Gruijters M J, Kramer P, Karels B, Ingraham H A, Nachtigal M W, Uilenbroek J T, Grootegoed J A and Themmen A P. 2002. Anti-Mullerian hormone inhibits initiation of primordial follicle growth in the mouse ovary. Endocrinology 143: 1076–84. DOI: https://doi.org/10.1210/endo.143.3.8691

Ginther O J, Wiltbank M C, Fricke P M, Gibbons J R and Kot K. 1996. Selection of the dominant follicle in cattle Biology of Reproduction 55: 1187–94 DOI: https://doi.org/10.1095/biolreprod55.6.1187

Jimenez-Krassel F, Scheetz, D, Neuder L, Ireland J, Pursley J, Smith G, Tempelman R, Ferris, T, Roudebush W and Mossa F. 2015. Concentration of anti-mullerian hormone in dairy heifers is positively associated with productive herd life. Journal of Dairy Science 98 3036–45 DOI: https://doi.org/10.3168/jds.2014-8130

Mondal S, Prakash B S, Palta P. 2007. Endocrine aspects of oestrous cycle in buffaloes (Bubalus bubalis): An overview. Asian Australasian Journal of Animal Science 20(1): 124–31. DOI: https://doi.org/10.5713/ajas.2007.124

Mondal S, Suresh K P and Nandi S. 2010. Endocrine profiles of oestrous cycle in buffalo: A meta-analysis. Asian Australasian Journal of Animal Science 23(2): 169–74. DOI: https://doi.org/10.5713/ajas.2010.90193

Monniaux D, Drouilhet L, Rico C, Estienne A, Jarrier P, Touze J L, Sapa J, Phocas F, Dupont J, Dalbies-Tran R and Fabre S. 2013. Regulation of anti- mullerian hormone production in domestic animals Reproduction, Fertility and Development 25 1–16 DOI: https://doi.org/10.1071/RD12270

Mossa F, Walsh S, Butler S, Berry D, Carter F, Lonergan P, Smith G, Ireland J and Evans A. 2012. Low numbers of ovarian follicle 3 mm in diameter are associated with low fertility in dairy cows. Journal of Dairy Science 95 2355–61 DOI: https://doi.org/10.3168/jds.2011-4325

Pfeiffer K E, Jury L J and Larson J E. 2014. Determination of anti-mullerian hormone at estrus during a synchronized and a natural bovine estrous cycle. Domestic Animal Endocrinology 46 58–64 DOI: https://doi.org/10.1016/j.domaniend.2013.05.004

Ribeiro E S, Bisinotto R S, Lima F S, Greco LF, Morrison A, Kumar A, Thatcher W W and Santos J E P. 2014. Plasma anti- mullerian hormone in adult dairy cows and associations with fertility. Journal of Dairy Science 97: 6888–900 DOI: https://doi.org/10.3168/jds.2014-7908

Rico C, Fabre S, Medigue C, di Clemente N, Clement F, Bontoux M, Touze J L, Dupont M, Briant E, Remy B, Beckers J F and Monniaux D. 2009. Anti-Mullerian hormone is an endocrine marker of ovarian gonadotropin responsive follicles and can help to predict superovulatory responses in the cow. Biology of Reproduction 80 50–59 DOI: https://doi.org/10.1095/biolreprod.108.072157

Rico C, Medigue C, Fabre S, Jarrier P, Bontoux M, Clement F and Monniaux D. 2011. Regulation of anti-mullerian hormone production in the cow: A multiscale study at endocrine, ovarian, follicular, and granulosa cell levels. Biology of Reproduction 84: 560–71 DOI: https://doi.org/10.1095/biolreprod.110.088187

Scheetz D M. 2010. ‘Regulation and role of anti-mullerian hormone in bovine reproduction.’ Thesis, Michigan State University.

Taylor C and Rajamahendran R. 1991. Follicular dynamics, corpus luteum growth and regression in lactating dairy cattle. Canadian Journal of Animal Science 71 61–68 DOI: https://doi.org/10.4141/cjas91-007

Warriach H M, Ahmad N. 2009. Follicular population during the oestrous cycle in Nili-Ravi buffaloes undergoing spontaneous and PGF2 induced luteolysis. Asian Australasian Journal of Animal Science 22(8): 1113–6. DOI: https://doi.org/10.5713/ajas.2009.90029

Yilmaz O, Yazici E, Kahraman A, Ozenc E and Ucar M. 2014. The relationship between ovarian follicle population and follicle size during different stages of estrous cycle in Anatolian water buffaloes (Bubalus bubalis). Revue De Medicine eterinaire 165(3–4): 111–15.