Heat stress amelioration by astaxanthin supplementation and Nrf2 transcribed downregulation of HSP70 and MnSOD in Karan Fries (Holstein Friesian ×Tharparkar) heifers

SUNIL KUMAR; S V SINGH

doi:10.56093/ijans.v89i11.95862

Authors

SUNIL KUMAR PhD Scholar, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
S V SINGH Principal Scientist, ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v89i11.95862

Keywords:

Antioxidant enzymes, Astaxanthin, Gene expression, Karan Fries, Summer stress

Abstract

Karan Fries (Holstein Friesian × Tharparkar) heifers are sensitive to heat stress due to exotic inheritance. Therefore their productive performances are affected adversely under tropical climatic conditions. Hence, the present study was conducted on Karan Fries (10-12 months) heifers by dividing into two group i.e. control (fed as per ICAR, 2013) and treatment group (additionally supplemented with astaxanthin @ 0.25 mg/kgBW/day/animal). Environmental parameters (dry and wet bulb temperature, and relative humidity) were recorded throughout the study and temperature humidity index (THI) was calculated. THI was used to assess the levels of summer stress. Blood samples were collected at fortnightly interval from both the groups of heifers for estimation of oxidative stress markers i.e. antioxidants enzyme (SOD, GPx and catalase). RNA was isolated from collected blood samples for Nrf2, HSP70 and MnSOD gene expression at monthly interval. Plasma catalase, SOD and GPx levels were lower in astaxanthin supplemented group of Karan Fries heifers than control group. The expression of HSP70 and MnSOD genes was down-regulated and Nrf2 was up-regulated in treatment compared to control group. The THI had a positive correlation with oxidative stress markers i.e. antioxidants enzyme (SOD, GPx & Catalase) as well as expression pattern of HSP70, and MnSOD genes. Based on the results of the present study, it can be concluded that astaxanthin (potent antioxidant) supplementation was effective in lowering the stress levels in treatment group by down-regulating the genes related to heat stress. Therefore, astaxanthin can be an effective supplement for ameliorating the adverse effect of heat stress, mainly in heat sensitive livestock species.

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References

Arya R, Mallik M and Lakhotia S C. 2007. Heat shock genes- integrating cell survival and death. Journal of Bioscience 32: 595–610.

Chandra G and Aggarwal A. 2009. Effect of DL- -tocopherol acetate on calving induced oxidative stress in periparturient crossbred cows during summer and winter seasons. Indian Journal of Animal Nutrition 26 204–10

Chaudhary S S, Singh V K, Upadhyay R C, Puri G, Odedara A B and Patel P A. 2015. Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes. Veteterinary World 8(7): 727–31.

Dröge W. 2002. Free radicals in the physiological control of cell function. Physiological Reviews 82 47–95

Fang Y, Zhong R, Chen L, Feng C, Sun H and Zhou D. 2015. Effects of astaxanthin supplementation on the sperm quality and antioxidant capacity of ram semen during liquid storage. Small Ruminant Research 130: 178–82.

Ganaie A H, Shanker G, Bumla N A, Ghasura R S and Mir N A. 2013. Biochemical and physiological changes during thermal stress in bovines. Journal of Veteterinary Science and Technololgy 4 1–6

Kobayashi A, Kang M I, Watai Y, Tong K I, Shibata T, Uchida K and Yamamoto M. 2006. Oxidative and electrophilic stresses activate Nrf2 through inhibition of ubiquitination activity of Keap1. Molecular Cell Biology 26(1): 221–29.

Kristinsson H G and Miyashita K. 2014. Marine antioxidants. polyphenols and carotene from algae. Antioxidant and functional components in aquatic foods. (Ed.) Hordur G. John Wiley & Sons, Ltd, Kristinsson.

Kumar M, Kaur H, Deka R S, Mani V, Tyagi A K and Chandra G. 2015. Dietary inorganic chromium in summer-exposed buffalo calves (Bubalus bubalis): effects on biomarkers of heat stress, immune status, and endocrine variables. Biological Trace Element Research 167(1): 18–27.

Kumar R, Gupta I D, Verma A, Singh S V, Verma N, Vineeth M R, Magotra A and Das R. 2016. Novel SNP identification in exon 3 of HSP90AA1 gene and their association with heat tolerance traits in Karan Fries cows under tropical climatic condition. Tropical Animal Health and Production 48: 735– 40

Lallawmkimi M C, Singh S V, Dandage S D, Vaidya M M and Upadhyay R C. 2010. Impact of vitamin E supplementation on antioxidants in thermal stressed Murrah buffaloes. Review Veterinary 21(1).

Lee D H, Kim C S and Lee Y J. 2011. Astaxanthin protects against MPTP/MPP +- induced mitochondrial dysfunction and ROS production n vivo and n vitro Food and Chemical Toxicology 49 271–80.

Li Z, Dong X, Liu H, Chen X, Shi H, Fan Y and Zhang X. 2013. Astaxanthin protects ARPE-19 cells from oxidative stress via upregulation of Nrf2-regulated phase II enzymes through activation of PI3K/Akt. Molecular Vision 19 1656–66

Liu G H, Qu J and Shen X. 2008. NF- B/p65 antagonizes Nrf2- ARE pathway by depriving CBP from Nrf2 and facilitating recruitment of HDAC3 to MafK. Molecular Cell Research 1783(5): 713–27.

Livak K J and Schnittgen T D. 2001. Analysis of relative gene expression data using real time quantitative PCR and the 2(- Delta Delta C (T)). Method 25 402–08

Maibam U, Hooda O K, Sharma P S, Mohanty A K, Singh S V and Upadhyay R C. 2017. Expression of HSP70 genes in skin of zebu (Tharparkar) and crossbred (Karan Fries) cattle during different seasons under tropical climatic conditions. Journal of Thermal Biology 63 58-64

Mishra A, Hooda O K, Singh G and Meur S K. 2010. Influence of induced heat stress on HSP70 in buffalo lymphocytes. Journal of Animal Physiology and Animal Nutrition 95 540– 44

Mustafi S B, Chakraborty P K, Dey R S and Raha S. 2009. Heat stress upregulates chaperone heat shock protein 70 and antioxidant manganese superoxide dismutase through reactive oxygen species (ROS), p38MAPK, and Akt. Cell Stress and Chaperones 14(6): 579.

Na H K and Surh Y J. 2008. Modulation of Nrf2-mediated antioxidant and detoxifying enzyme induction by the green tea polyphenol EGCG. Food and Chemical Toxicology 46(4): 1271–78.

Namekawa T, Ikeda S, Sugimoto M and Kume S. 2010. Effects of astaxanthin containing oil on development and stress related gene expression of bovine embryos exposed to heat stress. Reproduction in Domestic Animals 45(6): e387–91.

Reiter R J, Paredes L C, Manchester S D and Tan D X. 2009. Reducing oxidative/nitrosative stress: a newly-discovered genre for melatonin. Critical Reviews in Biochemistry and Molecular Biology 44(4): 175–200.

Ruell P A, Thompson M W and Hoffman K M. 2009. Heat shock proteins as an aid in the treatment and diagnosis of heat stroke. Journal of Thermal Biology 34(1): 1–7.

Sahin K, Orhan C, Smith M O and Sahin N. 2013. Molecular targets of dietary phytochemicals for the alleviation of heat stress in poultry. World’s Poultry Science Journal 69(1): 113– 24

Sahin K, Orhan C, Akdemir F, Tuzcu M, Iben C and Sahin N. 2012. Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins. Journal of Animal Physiology and Animal Nutrition 96(1): 66–74.

Sahin K, Orhan C, Akdemir F, Tuzcu T, Ali S and Sahin N. 2011. Tomato powder supplementation activates Nrf-2 via ERK/Akt signalling pathway and attenuates heat stress-related responses in quails. Animal Feed Science and Technology 165(3): 230– 37.

Sahin K, Orhan C, Tuzcu M, Ali S, Sahin N and Hayirli A. 2010. Epigallocatechin-3-gallate prevents lipid peroxidation and enhances antioxidant defense system via modulating hepatic nuclear transcription factors in heat-stressed quails. Poultry Science 89(10): 2251–58.

SAS Institute. 2011. Statistical Analysis System. Version 9.1. SAS Institute, Cary, NC, USA.

Sheikh A, Aggarwal A and Aarif O. 2016. Effect of n vitro zinc supplementation on HSPs expression and interleukin 10 production in heat treated peripheral blood mononuclear cells of transition Sahiwal and Karan Fries cows. Journal of Thermal Biology 56 68–76

Sheikh A A, Aggarwal A, Aarif O and Upadhyay R C. 2015. In vitro effect of zinc treatment on the antioxidant status of heat stressed peripheral blood mononuclear cells of periparturient sahiwal and karan fries cows - a comparative study. Journal of Animal Research 5(2): 243–49.

Shen G, Xu C, Hu R, Jain M R, Gopalkrishnan A, Nair S, Huang M T, Chan J Y and Kong A N. 2006. Modulation of nuclear factor E2-related factor 2-mediated gene expression in mice liver and small intestine by cancer chemopreventive agent curcumin. Molecular Cancer Therapeutics 5(1): 39–51.

Shibu C T, Singh S V and Upadhyay R C. 2008. Impact of temperature rise on pulmonary dynamics, heat dissipation and antioxidant in Karan Fries heifers. Indian Journal of Animal Nutrition 25: 67–71.

Shin M H, Moon Y J, Seo J E, Lee Y, Kim K H and Chung J H. 2008. Reactive oxygen species produced by NADH oxidase, Xanthine oxidase and mitochondrial electron transport system mediate heat shock-induced MMP-1 and MMP-9 expression. Free Radical Biology and Medicine 44 635–45

Singh A K, Upadhyay R C, Malakar D, Kumar S and Singh S V. 2014. Effect of thermal stress on HSP70 expression in dermal fibroblast of zebu (Tharparkar) and crossbred (Karan-Fries) cattle. Journal of Thermal Biology 43 46–53

Singh B, Ghuman S P S, Cheema R S and Bansal A K. 2016. Melatonin implant induces estrus and alleviates oxidative stress in summer anestrous buffalo. Indian Journal of Animal Reproduction 37(2): 28–32.

Soren S, Singh S V and Kumar S. 2017. Effect of astaxanthin supplementation on semen (Karan Fries bulls) storage at 5°C. International Journal of Current Microbiology and Applied Sciences 6(5): 23–28.

Sottile M L and Nadin S B. 2017. Heat shock proteins and DNA repair mechanisms: an updated overview. Cell Stress and Chaperones 23(3): 303–15.

Tan S M, Sharma A and deHaan J B. 2012. Oxidative stress and novel antioxidant approaches to reduce diabetic complications. Oxidative Stress and Diseases InTech Turrens J F. 2003. Mitochondrial formation of reactive oxygen species. Journal of Physiology 552 335–44

Wankar AK, Singh G and Yadav B. 2014. Thermoregulatory and adaptive responses of adult buffaloes (Bubalus bubalis) during hyperthermia: Physiological, behavioral and metabolic approach. Veterinary World 7 825–30

Xu J, Tang S, Song E, Yin B, Wu D and Bao E. 2017. Hsp70 expression induced by co-enzyme Q10 protected chicken myocardial cells from damage and apoptosis under n vitro heat stress. Poultry Sci n 96(5): 1426–37.

Yin B, Tang S, Sun J, Zhang X, Xu J, Di L and Bao E. 2018. Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress. Cell Stress and Chaperones 23(4): 735–48.