Detection and molecular characterization of chicken infectious anaemia virus in poultry flocks in Haryana

RAHUL BRAR; VANDNA BAHNOT; N K MAHAJAN; PIYUSH TOMAR; ANAND PRAKASH; SUSHILA MAAN

doi:10.56093/ijans.v89i12.96620

Authors

RAHUL BRAR MVSc Scholar, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
VANDNA BAHNOT ADIO, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
N K MAHAJAN Principal Scientist, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
PIYUSH TOMAR PhD Scholar, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
ANAND PRAKASH ADIO, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
SUSHILA MAAN Professor, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India

https://doi.org/10.56093/ijans.v89i12.96620

Keywords:

Chicken infectious anaemia, PCR, Poultry, Sequencing, VP2 gene

Abstract

Chicken infectious anaemia virus (CIAV) is an economically important pathogen affecting poultry industry worldwide. Commercial poultry flocks (26) suspected to be affected with chicken infectious anaemia (CIA) were processed for Polymerase Chain Reaction (PCR) using VP2 gene primers and characterized by partial gene sequencing. The PCR revealed 26.9% (7/26) poultry flocks positive for CIAV. Eleven PCR products of CIAV DNA were sequenced. The partial nucleotide sequence analysis of VP2 genes revealed that 11 field strains had 99.2 to 100% similarity among themselves and with the Indian strains. The VP2 gene sequences of 11 field strains showed 97.5 to 100% similarity to the field strains sequences reported from all over the world. On the basis of partial nucleotide sequencing analysis of VP2 gene, our findings suggest that the viral strains circulating in Haryana have similarity to other Indian strains.

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