Seroepidemiological survey of brucellosis and isolation of Brucella suis from swine herds of Meghalaya, North-East India

INGUDAM SHAKUNTALA; SANDEEP GHATAK; SAMIR DAS; AROCKIASAMYARUN PRINCE MILTON; RAJKUMARI SANJUKTA; KEKUNGU-U PURO; ANIRBAN DUTTA; KOUSHIK KAKOTY; AMARJIT KARAM; LALHRUAIPUII LALHRUAIPUII; ARNAB SEN

doi:10.56093/ijans.v90i1.98163

Authors

INGUDAM SHAKUNTALA ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
SANDEEP GHATAK ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
SAMIR DAS ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
AROCKIASAMYARUN PRINCE MILTON ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
RAJKUMARI SANJUKTA ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
KEKUNGU-U PURO ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
ANIRBAN DUTTA ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
KOUSHIK KAKOTY ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
AMARJIT KARAM ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
LALHRUAIPUII LALHRUAIPUII ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
ARNAB SEN ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

https://doi.org/10.56093/ijans.v90i1.98163

Keywords:

Bruce ladder PCR, Brucella, B. suis, Porcine brucellosis, Risk factors, Seroprevalence

Abstract

Brucellosis is a well-known and wide spread zoonotic disease. It is endemic in several parts of Asia, including India. In this study, seroprevalence of porcine brucellosis was studied among apparently healthy pigs in Meghalaya where pig keeping plays a significant function in socio-economic development. Serum samples (3,597) from pigs were screened using Rose Bengal Plate Test (RBPT) and indirect ELISA. Isolation of Brucella was attempted in clinical samples. A total of 13 (0.36%) were positive by RBPT and 72(2%) by Indirect ELISA. Brucella suis isolate was recovered from placenta of an aborted pig. Risk factors involved in the transmission of brucellosis amongst swine herd were studied. It was observed that age (OR=0.590; P=0.04) and sex (OR=0.557; P=0.04) were significant intrinsic risk factors for transmission of porcine brucellosis. Although the seroprevalence is low, isolation of B. suis from an aborted pig indicated that disease is actively circulating among swine herds of Meghalaya.

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Author Biographies

INGUDAM SHAKUNTALA, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
SANDEEP GHATAK, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
SAMIR DAS, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
AROCKIASAMYARUN PRINCE MILTON, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

Scientist, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
RAJKUMARI SANJUKTA, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
KEKUNGU-U PURO, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
ANIRBAN DUTTA, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
KOUSHIK KAKOTY, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
AMARJIT KARAM, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
LALHRUAIPUII LALHRUAIPUII, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India
ARNAB SEN, ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

ICAR-Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 India

References

Al Dahouk S, Tomaso H, Nockler K, Neubauer H and Frangoulidis D. 2003. Laboratory based diagnosis of Brucellosis—a review of the literature. Part II: Serological tests for brucellosis. Clinical Laboratory 49: 577–89.

Alton G G, Jones L M, Angus R D and Verger J M. 1988. Techniques for the brucellosis laboratory. 1st edition Paris: Institut National de laRecherche Agronomique.

Baily G G, Krahn J B, Drasar B S and Stoker N G. 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. Journal of Tropical Medicine and Hygiene 95: 271–75.

Bricker B J and Halling S M. 1994. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv.1 by PCR. Journal of Clinical Microbiology 32: 2660–66. DOI: https://doi.org/10.1128/jcm.32.11.2660-2666.1994

Chand P and Chhabra R. 2013. Herd and individual animal prevalence of bovine brucellosis with associated risk factors on dairy farms in Haryana and Punjab in India. Tropical Animal Health and Production 45: 1313–19. DOI: https://doi.org/10.1007/s11250-013-0362-y

Deyoe B L. 1986. Brucellosis. Diseases of Swine. 6th ed. Leman A D, Straw B, Glock R D, et al. Eds. Ames: Iowa State University Press pp. 599–607.

Diaz Aparicio E. 2013. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Revue Scientifique et Technique 32: 43–5, 53–60. DOI: https://doi.org/10.20506/rst.32.1.2187

Godfroid J, Nielsen K and Saegerman C. 2010. Diagnosis of brucellosis in livestock and wildlife. Croatian Medical Journal 51: 296–305. DOI: https://doi.org/10.3325/cmj.2010.51.296

Government of India (GOI). 2014. Basic Animal Husbandry Statistics. Ministry of Agriculture. Department of Animal Husbandry, Dairying and Fisheries. New Delhi.

Jindal P, Singh B B, Narang D and Gill J P S. 2016. Seroprevalence and molecular detection of Brucella species in pig producers of Punjab. Indian Journal of Animal Research 6: 835–41. DOI: https://doi.org/10.5958/2277-940X.2016.00106.6

Kadirvel G, Banerjee B B, Meitei S, Doley S, Sen A and Muthukumar M. 2018. Market potential and opportunities for commercialization of traditional meat products in North East hill region of India. Veterinary World 11: 118–24. DOI: https://doi.org/10.14202/vetworld.2018.118-124

Kebeta M M, Mamo G, Kassa T, Assaye M, Ashenafi H and Zewdu E. 2015. Seroprevalence of Brucellosis from Pigs: The First Report in Central Ethiopia. Journal of Veterinary Science and Technology 6: 2. DOI: https://doi.org/10.4172/2157-7579.1000215

Kumar R and Rao C V N. 1980. Porcine brucellosis in Tamil Nadu. Indian Veterinary Journal 57: 1–4.

Lopez-Goni I, Garcia-Yoldi D, Marin C M, de Miguel M J, Munoz P M, Blasco J M, Jacques I, Grayon M, Cloeckaert A, Ferreira A C, Cardoso R, Corrêa de Sá M I, Walravens K, Albert D and Garin-Bastuji B. 2008. Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of All Brucella species, including the vaccine strains. Journal of Clinical Microbiology 46: 3484–87. DOI: https://doi.org/10.1128/JCM.00837-08

Mathur T N. 1985. The epidemiology of brucellosis. In: Proceedings of the Brucellosis symposium and Annual Conference of the Indian Association of pathologists and Microbiologists (IAPM), Udaipur, India, 25 October, 1985, 9–10.

Naha K, Dasari S, Pandit V and Seshadri S. 2012. A rare case of seronegative culture-proven infection with Brucella suis. Australasian Medical Journal 5: 340–43. DOI: https://doi.org/10.4066/AMJ.2012.1177

Nath A J, Barman N N, Sarma D K, Das S K and Bora D P. 2009. Seroprevalence of Brucella and parvovirus infections in pigs Indian Veterinary Journal 86: 339–41.

Ngbede E O, Momoh A H, Bala R S, Madaki B D and Maurice N A. 2013. An Abattoir based study on serodiagnosis of swine brucellosis in Makurdi, Benue State, North Central Nigeria Journal of Advanced Veterinary Research 3: 57–59.

Radostits W, Gay C C, Hinchcliff K W and Constable P D. 2007 Veterinary Medicine. 10th edn. Elsevier Saunders, London.

Rahman M S, Nuruzzaman M, Ahasan M S, Sarker R R, Chakrabartty A, Nahar A, Uddin M J, Sarker M A S and Akhter L. 2012. Prevalence of brucellosis in pigs: The first report in Bangladesh. Bangladesh Journal of Veterinary Medicine 10: 75–80. DOI: https://doi.org/10.3329/bjvm.v10i1-2.15649

See W, Edwards W H, Dauwalter S, Almendra C, Kardos M D, Lowell J L, Wallen R, Cain S L, Holben W E and Luikart G. 2012. Yersinia enterolitica an unlikely cause of positive brucellosis tests in greater yellow stone ecosystem bison (Bison bison). Journal of Wildlife Diseases 48: 537–41. DOI: https://doi.org/10.7589/0090-3558-48.3.537

Shimshony A. 2009. Porcine brucellosis: Another common zoonosis in pigs. Zoonotic Infection Infectious Disease News 22: 14.

Shome R, Deivanai M, Shome B R, Rao N K and Prabhudas K. 2011. Serological and PCR confirmation of Brucella suis abortion in swine. Indian Veterinary Journal 88: 17–19.

Shome R, Triveni K, Shankaranarayana B S, Sahay S, Rao N K, Shome B R, Misri J and Rahman H. 2016. Record of porcine brucellosis in India by indigenously developed indirect ELISA. Asian Pacific Journal of Tropical Disease 6: 892–96. DOI: https://doi.org/10.1016/S2222-1808(16)61153-2

World Organisation for Animal Health (OIE). 2009. Porcine brucellosis. Manual of standards for diagnostic tests and vaccines, for terrestrial animals, OIE, Paris.

World Organization for Animal Health (OIE). 2011. Chapter 2.8.5 Porcine Brucellosis. Manual for diagnosis tests and vaccines for terrestrial animals OIE, Paris.