Isolation and immunoblotting of somatic antigens of Cotylophoron cotylophorum and Gastrothylax crumenifer

R VIJAYASHANTHI; C SOUNDARARAJAN; M RAMAN; T M A SENTHILKUMAR

doi:10.56093/ijans.v90i1.98173

Authors

R VIJAYASHANTHI Junior Research Fellow, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
C SOUNDARARAJAN Professor, Department of Veterinary Parasitology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
M RAMAN Professor and Project Director, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
T M A SENTHILKUMAR Professor, Department of Animal Biotechnology, Madras Veterinary College, Chennai

https://doi.org/10.56093/ijans.v90i1.98173

Keywords:

Paramphistomes, Somatic antigen, SDS-PAGE, Western blot

Abstract

Paramphistomosis is the emerging fluke infection in young ruminants which results in high morbidity and mortality in tropics and sub-tropics. The present study was carried out to detect the sharing antigens among the purified somatic antigens of G. crumenifer and C. cotylophorum, in order to use as a candidate antigen for diagnosis of paramphistomosis in sheep. Immunoblot analysis of the purified somatic antigens of C. cotylophorum and G. crumenifer using their specific rabbit hyper immune serum revealed 3 common polypeptides at the range of 27, 29 and 60 kDa. Hence these three immunodominant peptides canÂ be used candidate antigens in development of vaccines against ovine paraphistomosis and also for early diagnosis of infection.

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Author Biographies

R VIJAYASHANTHI, Junior Research Fellow, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

Junior Research Fellow,Â Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
C SOUNDARARAJAN, Professor, Department of Veterinary Parasitology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

Professor, Department of Veterinary Parasitology,Â Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
M RAMAN, Professor and Project Director, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

Professor and Project Director,Â Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
T M A SENTHILKUMAR, Professor, Department of Animal Biotechnology, Madras Veterinary College, Chennai

Professor, Department of Animal Biotechnology, Madras Veterinary College, Chennai.

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