Multiple drug resistant Enterococcus spp. causes disease and mortality in Zebra fish (Danio rerio)

A UMA; A H M HARRESH; G REBECCA; J PRAVEENRAJ

doi:10.56093/ijans.v90i1.98241

Authors

A UMA Professor and Head, Tamil Nadu Dr J Jayalalitha Fisheries University, Madhavaram Milk Colony, Chennai, Tamil Nadu 600 051 India
A H M HARRESH Senior research Fellow, State Referral Laboratory for Aquatic Animal Health
G REBECCA Senior research Fellow, State Referral Laboratory for Aquatic Animal Health
J PRAVEENRAJ Scientist, Indian Council of Agricultural–Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands.

https://doi.org/10.56093/ijans.v90i1.98241

Keywords:

Antibiotics, 16S rRNA, Drug resistance, Model fish, Pathogens Present address, 1Professor and Head (uma@tnfu.ac.in), 2, 3Senior research

Abstract

The present report describes the isolation and identification of a multiple drug resistant Enterococcus spp. from diseased zebrafish from a commercial rearing facility in Chennai, Tamil Nadu, India. Zebrafish (Danio rerio) has recently gained great significance as a vertebrate animal model, as its immune system is remarkably similar with that of the humans. However, zebrafish are still susceptible to microbial infection. Gram positive diplococci isolated from kidney was identified as Enterococcus spp. using 16S rRNA gene sequencing analysis. The Enterococcus spp. isolate was either resistant and or intermediately resistant to 14 antibiotics assessed by agar disc diffusion method. This communication is the first report on isolation and confirmation of Enterococcus spp. associated with disease and mortality in zebrafish.

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Author Biographies

A UMA, Professor and Head, Tamil Nadu Dr J Jayalalitha Fisheries University, Madhavaram Milk Colony, Chennai, Tamil Nadu 600 051 India

Professor and Head
A H M HARRESH, Senior research Fellow, State Referral Laboratory for Aquatic Animal Health

Senior research Fellow, State Referral Laboratory for
Aquatic Animal Health,
G REBECCA, Senior research Fellow, State Referral Laboratory for Aquatic Animal Health

Senior research Fellow, State Referral Laboratory for Aquatic Animal Health,
J PRAVEENRAJ, Scientist, Indian Council of Agricultural–Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands.

Scientist, Indian
Council of Agriculturalâ€“Central Island Agricultural Research
Institute, Port Blair, Andaman and Nicobar Islands.

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