Analysis of biomarkers for prediction of idiopathic infertility in Large White Yorkshire boar (Sus scrofa domesticus)

SOMRAJ CHATTARAJ; SIDDHARTHA BASU; KALYANI RAY; SANJOY DATTA; PRADIP SARKAR; DURGADAS MANDAL

doi:10.56093/ijans.v90i2.98770

Authors

SOMRAJ CHATTARAJ Veterinary Officer, Animal Resource Development Department, Govt. of West Bengal
SIDDHARTHA BASU Professor, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, West Bengal 700 037 India
KALYANI RAY Professor, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, West Bengal 700 037 India
SANJOY DATTA Assistant Professor, Department of Animal Genetics and Breeding, WBUAFS, Kolkata
PRADIP SARKAR Assistant Professor, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, West Bengal 700 037 India
DURGADAS MANDAL Assistant Professor, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, West Bengal 700 037 India

https://doi.org/10.56093/ijans.v90i2.98770

Keywords:

Biomolecules, Curve fit regression equations, Epididymal lumen fluid (ELF), Sperm cytosolic fluid (SCF)

Abstract

The experiment was conducted to analyze biomarkers like pH, calcium (Ca²⁺), cholesterol (Ch), estradiol (E2) and vitamin D (Vit-D) in the serum for prediction of idiopathic infertility and its early amelioration. pH of epididymal luminal fluids (ELF) and concentrations of Ca²⁺, Ch, E2 and Vit-D were measured in serum, as well as in epididymal luminal fluids (ELF) and sperm cytosolic fluids (SCF) from caput, corpus and cauda epididymis of healthy, mature Large Yorkshire breed of boar (n=100). The levels of these bio-molecular components in the serum, in ELF and SCF from different epididymal segments were used statistically to develop a Curve Fit Regression Equations and a prediction equation to predict the quantum of these bio-molecules in the ELF and SCF by estimating their levels in the serum which would have been very difficult otherwise to be estimated in live animals. Further, from the clinical point of view, present findings about the spermatozoa maturation can pave the way for development of specific marker(s) that will assist in the development of new arena for both the prediction and early diagnosis of male infertility and for improving treatment modalities of male infertility, since epididymal dysfunctions are related to cases of idiopathic male infertility.

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