Comparative efficacy of conventional stains for evaluation of plasmalemma and acrosome integrity of buffalo spermatozoa

RUPALI RAUTELA; AMARJEET BISLA; ATHANAS ALEX NGOU; ABHISHEK KUMAR; S K GHOSH; N SRIVASTAVA

doi:10.56093/ijans.v90i2.98775

Authors

RUPALI RAUTELA PhD Scholar, ICAR-Indian Veterinary Research Institute Izatnagar, Uttar Pradesh 243 122 India
AMARJEET BISLA MVSc Scholar, ICAR-Indian Veterinary Research Institute Izatnagar, Uttar Pradesh 243 122 India
ATHANAS ALEX NGOU MVSc Scholar, ICAR-Indian Veterinary Research Institute Izatnagar, Uttar Pradesh 243 122 India
ABHISHEK KUMAR PhD Scholar, ICAR-Indian Veterinary Research Institute Izatnagar, Uttar Pradesh 243 122 India
S K GHOSH Principal Scientist, ICAR-Indian Veterinary Research Institute Izatnagar, Uttar Pradesh 243 122 India
N SRIVASTAVA Principal Scientist, ICAR-Indian Veterinary Research Institute Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v90i2.98775

Keywords:

Buffalo, Semen, Trypan blue, Giemsa, Eosin-Nigrosin

Abstract

The aim of the present study was to compare the efficiency of various conventional single and dual staining protocols for assessment of spermatozoa attributes. The efficiency of single stains such as Eosin-Nigrosin (EN) and Trypan blue (TB) for viability and Giemsa (G) for acrosome integrity vis-a-vis dual stains such as Trypan blue-Giemsa (TB-G) and Eosin Nigrosin-Giemsa (EN-G) were compared. Ejaculates (12) from 3 buffalo bulls were used for evaluation of livability and acrosome integrity using said stains. The smears stained with EN-G were difficult to visualize distinctly and provided obscure results. A nonsignificant difference was found between EN, TB and TB-G for spermatozoa viability. However, at recommended concentration, TB yielded faint images, thus making the observation unnecessarily tedious. However, staining with TB-G was found advantageous for simultaneous determination of viability and acrosome status. Moreover, the results between G and TB-G deferred nonsignificantly indicating similar effectiveness of both the stains. Overall, TB-G can be a reliable, quick and convenient method for determining viability and acrosome integrity simultaneously for routine semen evaluation.

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