Feed safety evaluation for prevalence of zoonotic Salmonella spp. in animal feed

V SHARMA; S SHARMA; A VERMA; D K DAHIYA; M KARNANI

doi:10.56093/ijans.v90i1.98937

Authors

V SHARMA Professor, Post Graduate Institute of Veterinary Education and Research, Jamdoli, Jaipur, Rajasthan 302 031 India
S SHARMA Professor, Post Graduate Institute of Veterinary Education and Research, Jamdoli, Jaipur, Rajasthan 302 031 India
A VERMA Teaching Associate, Post Graduate Institute of Veterinary Education and Research, Jamdoli, Jaipur, Rajasthan 302 031 India
D K DAHIYA Research Associate, Post Graduate Institute of Veterinary Education and Research, Jamdoli, Jaipur, Rajasthan 302 031 India
M KARNANI Assistant Professor, Post Graduate Institute of Veterinary Education and Research, Jamdoli, Jaipur, Rajasthan 302 031 India

https://doi.org/10.56093/ijans.v90i1.98937

Keywords:

Animal feed, Feed mills, PCR, Salmonella enteritidis, Salmonella typhimurium, Virulence genes

Abstract

Owing to the zoonotic nature of Salmonella, its transmission from feed-to-food is quite feasible and considered as one of the prime factors for the transmission and spread of virulent and drug-resistant strains in humans. Therefore, the present study was designed to investigate the prevalence of Salmonella in animal feed pellets from different feed mills in Jaipur and its nearby areas. For this, isolation of Salmonella was performed as per standard ISO methods and the presumable strains were further confirmed and characterized into different species by molecular methods. The confirmed strains were analyzed for virulence genes by PCR. Finally, the strains were analyzed for antimicrobial drug resistance by the standard disk diffusion method. The study revealed that the prevalence of Salmonella in feed pellets was moderate and Salmonella enteritidis and Salmonella typhimurium were the two dominated species. Strikingly, the majority of strains were found to possess the virulence genes and resistant to analyzed clinical antimicrobials. Results inferred that contaminated animal feeds may act as a potential source for the dissemination of virulent and drug-resistant Salmonella spp in animals as well as human beings. The present study implicated the need for more focused and extensive investigations from different parts of the country and the world for strict regulation of animal feed safety to assure one health concept.

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References

Amini K, Salehi T Z, Nikbakht G, Ranjbar R, Amini J and Ashrafganjooei S B. 2010. Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran. African Journal of Microbiology Research 4: 2202–10.

Burns A M, Lawlor P G, Gardiner G E, McCabe E M, Walsh D, Mohammed M, Grant J and Duffy G. 2015. Salmonella occurrence and Enterobacteriaceae counts in pig feed ingredients and compound feed from feed mills in Ireland. Preventive Veterinary Medicine 121: 231–39. DOI: https://doi.org/10.1016/j.prevetmed.2015.07.002

Chaudhary J, Nayak J, Brahmbhatt M N and Makwana P P. 2015. Virulence genes detection of Salmonella serovars isolated from pork and slaughterhouse environment in Ahmedabad, Gujarat. Veterinary World 8: 121. DOI: https://doi.org/10.14202/vetworld.2015.121-124

Clinical and Laboratory Standards Institute (CLSI). 2015. Performance Standards for Antimicrobial Susceptibility Testing: Twenty-fifth Informational Supplement M100-S25. Wayne, PA, USA.

Dahiya D K and Puniya A K. 2015. Evaluation of survival, free radical scavenging and human enterocyte adherence potential of lactobacilli with anti obesity and anti inflammatory cla isomer producing attributes. Journal of Food Processing and Preservation 39: 2866–77. DOI: https://doi.org/10.1111/jfpp.12538

Dahiya D K and Puniya A K. 2017. Isolation, molecular characterization and screening of indigenous lactobacilli for their abilities to produce bioactive conjugated linoleic acid (CLA). Journal of Food Science and Technology 54: 792– 801. DOI: https://doi.org/10.1007/s13197-017-2523-x

EFSA and ECFD (European Food Safety Authority and European Centre for Disease Prevention and Control). 2018. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA Journal 16: e05500. DOI: https://doi.org/10.2903/j.efsa.2018.5500

Elmadiena M M, El Hussein A A, Muckle C A, Cole L, Wilkie E, Mistry K and Perets A. 2013. Antimicrobial susceptibility and multi-drug resistance of Salmonella enterica subspecies enterica serovars in Sudan. Tropical Animal Health And Production 45: 1113–18. DOI: https://doi.org/10.1007/s11250-012-0334-7

Hsieh Y C, Poole T L, Runyon M, Hume M and Herrman T J. 2016. Prevalence of nontyphoidal Salmonella and Salmonella strains with conjugative antimicrobial-resistant serovars contaminating animal feed in Texas. Journal of Food Protection 79: 194–204. DOI: https://doi.org/10.4315/0362-028X.JFP-15-163

Koyuncu S and Haggblom P. 2009. A comparative study of cultural methods for the detection of Salmonella in feed and feed ingredients. BMC Veterinary Research 5: 6. DOI: https://doi.org/10.1186/1746-6148-5-6

Lopes G V, Pissetti C, Pellegrini D D C P, Silva L E D and Cardoso M. 2015. Resistance phenotypes and genotypes of Salmonella enterica subsp. enterica isolates from feed, pigs, and carcasses in Brazil. Journal of Food Protection 78: 407–13. DOI: https://doi.org/10.4315/0362-028X.JFP-14-274

Magossi G, Cernicchiaro N, Dritz S, Houser T, Woodworth J, Jones C and Trinetta V. 2018. Evaluation of Salmonella presence in selected United States feed mills. Microbiology Open 0: e00711. DOI: https://doi.org/10.1002/mbo3.711

Magouras I, Carmo L P, Stärk K D and Schüpbach-Regula G. 2017. Antimicrobial usage and-resistance in livestock: where should we focus? Frontiers in Veterinary Science 4: 148. DOI: https://doi.org/10.3389/fvets.2017.00148

Oliveira S, Santos L R, Schuch D M, Silva A B, Salle C T and Canal C W. 2002. Detection and identification of Salmonella from poultry-related samples by PCR. Veterinary Microbiology 87: 25–35. DOI: https://doi.org/10.1016/S0378-1135(02)00028-7

Pellegrini D D C P, Paim D S, Lima G J M M, Pissetti C, Kich J D and Cardoso M R D I. 2015. Distribution of Salmonella clonal groups in four Brazilian feed mills. Food Control 47: 672–78. DOI: https://doi.org/10.1016/j.foodcont.2014.08.013

Sharma V and Sharma S. 2015. Potential hazards in animal feeds: safety and regulation. Indian Journal of Animal Nutrition 32: 242–62.

Sharma S, Sharma V, Jain J, Agrawal S, Dahiya D K and Khan A. 2016. Organochlorine, organophosphate, synthetic pyrethroids assessment in bovine milk and water samples of Jaipur, Rajasthan. Indian Journal of Animal Sciences 86: 54–61.

Sharma S, Sharma V, Dahiya D K, Khan A, Mathur M and Sharma A. 2017a. Prevalence, virulence potential, and antibiotic susceptibility profile of Listeria monocytogenes isolated from bovine raw milk samples obtained from Rajasthan, India. Foodborne Pathogens and Disease 14: 132– 140. DOI: https://doi.org/10.1089/fpd.2016.2118

Sharma V, Sharma S, Dahiya D K, Khan A, Mathur M and Sharma A. 2017b. Coagulase gene polymorphism, enterotoxigenecity, biofilm production, and antibiotic resistance in Staphylococcus aureus isolated from bovine raw milk in North West India. Annals of Clinical Microbiology And Antimicrobials 16: 65. DOI: https://doi.org/10.1186/s12941-017-0242-9

Sharma V and Sharma S. 2017c. Feeding safe feed to livestock safeguards human health. Broadening Horizons, Feedipedia: 1–6.

Smith S I, Fowora M A, Atiba A, Anejo-Okopi J, Fingesi T, Adamu M E, Omonigbehin E A, Ugo-Ijeh MI, Bamidele M and Odeigah P. 2015. Molecular detection of some virulence genes in Salmonella spp isolated from food samples in Lagos, Nigeria. Animal and Veterinary Sciences 3: 22–27. DOI: https://doi.org/10.11648/j.avs.20150301.15

Tang K L, Caffrey N P, Nóbrega D B, Cork S C, Ronksley P E, Barkema H W, Polachek A J, Ganshorn H, Sharma N, Kellner J D and Ghali W A. 2017. Restricting the use of antibiotics in food-producing animals and its associations with antibiotic resistance in food-producing animals and human beings: a systematic review and meta-analysis. Lancet Planetary Health 1: e316–27. DOI: https://doi.org/10.1016/S2542-5196(17)30141-9

Yang S, Wu Z, Lin W, Xu L, Cheng L and Zhou L. 2017. Investigations into Salmonella contamination in feed production chain in Karst rural areas of China. Environmental Science and Pollution Research 24: 1372–79. DOI: https://doi.org/10.1007/s11356-016-7868-6